50-45-3 | Sigma-Aldrich

base pairs -60-200 µm 3-14 50 cm H2O 1.25 mL/min 15 mL/cm2h 6X104-2X107 3X104-5X106 -872 base pairs -45-165 µm

purified water. • Add 5 mL of this solution to 1 L of the melted mix (step 1) at 45-50 °C. • Stir well for homogenization • Step 3: • Pour into sterile Petri dishes. • Let it solidify and

Plant Growth

E N Indole-3-butyric acid (4-(3-Indolyl)butyric acid) CAS No. 133-32-4 C12H13NO2 FW 203.2 Plant cell culture, tested R: 25-36/37/38 S: 26-37-45 5 g 25 g I 7512 E Indole-3-butyric acid potassium

Mobius®CellReady 50 L and 200 L Single-use Bioreactor Systems Performance Guide

140 160 180 200 220 W /m 3 200 L Power/Volume Impeller rpm Impeller rpm 0 40 rpm [1 W/m3] 75 rpm [8 W/m3] 110 rpm [25 W/m3] 5 10 15 20 25 30 35 40 45 50 Ti m e [s ec ] 200

Steam-in-Place Sterilization of Filtration Trains containing Milligard®PES Prefilters

. 0.0 20.0 40.0 60.0 80.0 100.0 120.0 140.0 0 605 10 15 20 25 30 35 40 45 50 55 65 70 75 80 85 90 95 T em

IFU Date of Issue 2012-02

Cat. No. SLGVS25XS (0.2 µm, 50/box) Dualex™-Plus Transducer Protector Cat. No. SLGVS25PS (0.2 µm, 50/box) Dualex™-Ultra Transducer Protector Cat. No. SLGVS25US (0.2 µm, 50/box) ■ Single use only

Millipore Express® Family Guide

50 55 5 10 15 20 25 30 35 40 30 35 25 TT TB HH TH 0 20 0 gpm ps id 4.0 3.5 3.0

Product Information Sheet - CL4B200

base pairs -60-200 µm 3-14 50 cm H2O 1.25 mL/min 15 mL/cm2h 6X104-2X107 3X104-5X106 -872 base pairs -45-165 µm

Stericup®E and Steritop®E User Guide

base of the device then screw the filter into the top of a glass or plastic bottle with a 45 mm neck size. 3. Add all additional components to your media bottle. 4. Attach the Stericup® E or

Product Information Sheet - D2947

for 50 µL reaction volumes but can effectively purify samples from 45- 55 µL. • For PCR volumes > 55 µL, aliquot 50 µL into each tube for purification. • For PCR volumes 45 µL, dilute to 50 µL.

Stericup and Steritop Filtration and Storage Systems

50 m 40 30 20 100 0 0 0 0 400 100 200 300 If using the Steritop® filter funnel, screw it onto the top of a

Product Information Sheet - MAK443

of the 250 M L-Lactate and 45 L of Sample to separate wells of the plate. 3. For the Sample wells (See Sample Preparation, Step 3b), add 5 L of purified water and 45 L of Sample

Speed meets performance! Your first choice for ultra-fast HPLC - Purospher STAR RP-18 endcapped UHPLC columns

(2 µm) 50-2.1 mm Column: LiChroCART® 150-4.6 Purospher® STAR RP-18 endcapped, 5 µm Temperature: 40 °C Mobile phase: A: Water B: Acetonitrile Gradient: 0 min 45 % B, from 45 to

Product Information Sheet - 11810740001

bottle 3) for 10 min at +15 to +25°C. 6.1.4 Immuno- detection Sediment cells (300 × g, 10 min) and resuspend pellet in 50 �l anti-BrdU-FLUOS antibody working solution (II). Incubate for 45 min at

Product Information Sheet - 11939823001

Elongation time 45 sec 1 min 2 min 3 min 4 min Temperature for RT-step [°C] ≤60 50 50 48 45 – 48 (3) The cycle number is dependent on the abundance of the respective mRNA. For rare messages

Examination of Non-Specifically Bound Proteins and Peptides Following Depletion of Twenty High Abundance Proteins from Human Plasma

35 40 45 50 55 60 65 70 Time (minutes) A2 80 (m AU ) Flow-thru (Depleted Plasma) Eluted (Bound Proteins) Injected (0.30 ml/min) Elution (3 ml/min) Reequilibration (3 ml/min

Amlodipine Besylate and Related Substances From Particulate to Monolithic Column

maximum 2.5 μm (50% since the first method uses a 5 μm particle in the written standard operating procedure - SOP) or use a monolithic column • Shortening the column to a length of 45 mm (70%)

MultiScreen Solubility Filter Plate: Determination of aqueous compound solubility using a 96-well filter plate.

200 200 50 50 12.5 12.5 3.13 3.13 0 0 500 500 200 200 50 50 12.5 12.5 3.13 3.13 0 0 500 500 200 200 50 50 12.5 12.5 3.13 3.13 0 0 500 500 200

Product Information Sheet-ftaq-ro

Pre-Incubation/activation 95 4 min(1) 1 Denaturation Annealing Elongation 95 45 – 65(3) 72 30 sec 30 sec 45 sec – 3 min(4) 30 – 40(2) Final Elongation 72 7 min 1 Cooling 4 indefinitely

Product Information Sheet-11585606910

2 5 1 495 1:100 10 pg/μl 3 15 2 35 1:3.3 3 pg/μl 4 5 2 45 1:10 1 pg/μl 5 5 3 45 1:10 0.3 pg/μl 6 5 4 45 1:10 0.1 pg/μl 7 5 5 45 1:10 0.03 pg/μl 8 5 6 45

Ethacrynic Acid and Related Compounds - From Particulate to Monolithic Column

Gradient: See Table 100 O CH2 COOH Temperature: 50 °C Diluent:

Data Sheet - 67631

water bath at 45-50 °C, swirling or stirring gently. Advice 2: In case of samples with a high presence of Aeromonas, 50 mg of cefsulodin can be added to the mix once cooled down at 45-50 °C (50 mg/L).

IFU Date of Issue 2012-08

Millex® filter inlet and invert vial assembly. OR Millex® filter 3 4 5 English Millex® Vial Vent Cat. No. SLGVS25LS (50/Box) ■ Single use only ■ Sterile ■ Non-pyrogenic Applications

Combat Microbes with ProClin Biocidal Preservatives

salts 3% Mg and Cu salts Alkyl Carboxylate (salt-free) None Matrix Water Water Modified glycol Water Working pH Range 2.5 - 8.5 2.5 - 8.5 2.5 - 8.5 2 - 12 Temperature Range 45 °C

Antibiotics and Antimycotics

bacteria, and mycoplasma. Potency: approx.600 mg gentamicin per mg R: 42/43 S: 22-36/37-45 Hygromycin B 50 mg 100 mg 250 mg H 9773 E N from Streptomyces hygroscopicus CAS No. 31282-04-9

PyroDetect Cryoblood Shipment Validation Summary

35 -35 40 40 -40 -45 -50 -55 -60 -65 -70 -75 -85 TEMECULA Probe 1 Te m p er at u re ( °C )

Product Information Sheet - D2947L

for 50 µL reaction volumes but can effectively purify samples from 45- 55 µL. • For PCR volumes > 55 µL, aliquot 50 µL into each tube for purification. • For PCR volumes 45 µL, dilute to 50 µL.

Millipore Express®SHR Hydrophilic Filters

50 55 5 10 15 20 25 30 35 40 30 35 25 TT TB HH TH 0 20 0 gpm ps id 4.0 3.5 3.0

Data Sheet - SCC315

Age 45 (Prep 83-C) Cat # SCC315 antibodies Multiplex products biotools cell culture

Sterilizing-grade Durapore®Multimedia Filters

ps id 35 10 5 15 20 25 30 40 45 50 0 3.0 3.5 2.5 2.0 1.5 1.0 0.5 0.0