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Showing 1-30 of 199 results for "50933" within Papers
Sietse T Braakman et al.
Experimental eye research, 130, 87-96 (2014-12-03)
All aqueous humor draining through the conventional outflow pathway must cross the endothelium of Schlemm's canal (SC), likely by passing through micron-sized transendothelial pores. SC pores are non-uniformly distributed along the inner wall endothelium, but it is unclear how the
Angireddy Rajesh et al.
Journal of reproductive immunology, 110, 117-129 (2015-04-23)
Evaluating the immunocontraceptive potential of sperm-bound proteins is an active area of investigation. In this study, we analyzed the role of prostate- and testes-expressed (PATE) and PATE-F proteins in sperm function. Capacitation was measured as a function of tyrosine phosphorylation
Magali Solé et al.
FEBS letters, 587(6), 737-742 (2013-02-12)
Unfolding by chemical denaturants and the linear extrapolation method are widely used to determine the free energy of proteins. Ribonuclease 3 from bullfrog shows an extraordinary behavior in guanidinium hydrochloride in comparison to its homologues ribonuclease A and onconase with
Fabian U Zwettler et al.
Nature communications, 11(1), 3222-3222 (2020-06-28)
The synaptonemal complex (SC) is a meiosis-specific nuclear multiprotein complex that is essential for proper synapsis, recombination and segregation of homologous chromosomes. We combined structured illumination microscopy (SIM) with different expansion microscopy (ExM) protocols including U-ExM, proExM, and magnified analysis
Sujit Sadashiv Jagtap et al.
Applied and environmental microbiology, 80(14), 4207-4214 (2014-05-06)
Marine microbes use alginate lyases to degrade and catabolize alginate, a major cell wall matrix polysaccharide of brown seaweeds. Microbes frequently contain multiple, apparently redundant alginate lyases, raising the question of whether these enzymes have complementary functions. We report here
Keitaro Hirawatari et al.
Mammalian genome : official journal of the International Mammalian Genome Society, 26(5-6), 248-256 (2015-05-13)
Infertility in humans and subfertility in domestic animals are two major reproductive problems. Among human couples, ~15% are diagnosed as infertile, and males are considered responsible in about 50% of the cases. To examine male fertility, various sperm tests including
James A Woo et al.
Journal of chromatography. A, 1407, 58-68 (2015-07-15)
A homologous ligand library based on the commercially-available Nuvia cPrime ligand was generated to systematically explore various features of a multimodal cation-exchange ligand and to identify structural variants that had significantly altered chromatographic selectivity. Substitution of the polar amide bond
Zhihua Ren et al.
PloS one, 9(9), e107823-e107823 (2014-09-18)
Angiogenesis has become an attractive target in cancer treatment. Endostatin is one of the potent anti-angiogenesis agents. Its recombinant form expressed in the yeast system is currently under clinical trials. Endostatin suppresses tumor formation through the inhibition of blood vessel
J-K Ryu et al.
Andrology, 1(2), 216-222 (2013-01-15)
A prerequisite for the successful clinical application of gene therapy in erectile dysfunction (ED) is the availability of safe and efficient gene delivery systems. The aim of this study was to examine the effectiveness of guanidinylated bioreducible polymer (GBP) polyplexes
Zhongxin Zhou et al.
Biotechnology and applied biochemistry, 62(2), 268-274 (2014-06-04)
More information regarding the bactericidal properties of polyhexamethylene guanidine hydrochloride (PHMG) against clinically important antibiotic-resistant ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens needs to be provided for its uses in infection control.
Timothy J Brazil et al.
Veterinary journal (London, England : 1997), 202(3), 536-542 (2014-09-23)
The aim of this study was to characterise constitutive apoptosis in equine peripheral blood neutrophils, including assessment of factors that potentially modulate neutrophil survival through alteration of the rate of constitutive apoptosis. Cells underwent spontaneous time-dependent constitutive apoptosis when aged
Tian Wang et al.
Journal of pharmaceutical and biomedical analysis, 102, 519-528 (2014-12-03)
Disulfide reduction in therapeutic monoclonal antibodies can occur during cell harvest operations as a result of cell breakage. Understanding these product quality changes and manufacturers' ability to control them would likely be of concern to regulatory bodies. To study the
Allan Joaquim Lamontanara et al.
Nature communications, 5, 5470-5470 (2014-11-18)
The activity of protein kinases is regulated by multiple molecular mechanisms, and their disruption is a common driver of oncogenesis. A central and almost universal control element of protein kinase activity is the activation loop that utilizes both conformation and
Lodi C W Roksnoer et al.
Kidney international, 88(1), 109-120 (2015-04-02)
Neprilysin inhibitors prevent the breakdown of bradykinin and natriuretic peptides, promoting vasodilation and natriuresis. However, they also increase angiotensin II and endothelin-1. Here we studied the effects of a low and a high dose of the neprilysin inhibitor thiorphan on
Neelesh Singh et al.
Applied biochemistry and biotechnology, 169(8), 2315-2325 (2013-03-01)
Physiologically as well as industrially, α-galactosidases are very important enzymes, but very little is known about the stability and folding aspect of enzyme. In the present study, we have investigated the temperature, pH, and guanidine hydrochloride (GuHCl) induced unfolding of
Elamaran Meibalan et al.
Eukaryotic cell, 14(4), 371-384 (2015-02-11)
Malaria parasites replicating inside red blood cells (RBCs) export a large subset of proteins into the erythrocyte cytoplasm to facilitate parasite growth and survival. PTEX, the parasite-encoded translocon, mediates protein transport across the parasitophorous vacuolar membrane (PVM) in Plasmodium falciparum-infected
Madhurima S Wakankar et al.
International journal of biological macromolecules, 56, 149-155 (2013-03-15)
The Cicer arietinum seed lectin was cloned and expressed in Escherichia coli and purified in active form. Conformational characterization of the recombinant lectin (rCAL) was performed using biophysical and bioinformatics tools. Thermal denaturation of rCAL caused rapid secondary structural rearrangements
Weronika E Borek et al.
Nature communications, 6, 7929-7929 (2015-08-06)
Microtubule nucleation is highly regulated during the eukaryotic cell cycle, but the underlying molecular mechanisms are largely unknown. During mitosis in fission yeast Schizosaccharomyces pombe, cytoplasmic microtubule nucleation ceases simultaneously with intranuclear mitotic spindle assembly. Cytoplasmic nucleation depends on the
Jennifer A Boylston et al.
Journal of molecular and cellular cardiology, 88, 73-81 (2015-09-22)
Succinylation refers to modification of lysine residues with succinyl groups donated by succinyl-CoA. Sirtuin5 (Sirt5) is a mitochondrial NAD(+)-dependent deacylase that catalyzes the removal of succinyl groups from proteins. Sirt5 and protein succinylation are conserved across species, suggesting functional importance
Qi Chen et al.
Analytical and bioanalytical chemistry, 407(3), 1027-1032 (2014-10-20)
To analyze the proteome of an extremely low number of cells or even a single cell, we established a new method of digesting whole cells into mass-spectrometry-identifiable peptides in a single step within 2 h. Our sampling method greatly simplified the
Shin Koike et al.
Biochemical and biophysical research communications, 467(2), 361-366 (2015-10-04)
Although it's well known that protein carbonyl (PCO) and advanced glycation end-products (AGEs) levels are elevated in plasma from patients with renal dysfunction, we recently identified patients who had no renal dysfunction but possessed high levels of plasma pentosidine (PEN)
Amanda Phaelante et al.
Journal of cardiovascular translational research, 8(5), 328-337 (2015-06-19)
The antioxidant N-acetycysteine can turn into a prooxidant molecule in presence of iron ions. Thus, our goal was to test if the association of N-acetylcysteine (NAC) and an iron chelator (deferoxamine--DFX) in a rodent model of acute myocardial infarction (AMI)
Crispin G Alexander et al.
Biochimica et biophysica acta, 1844(12), 2241-2250 (2014-09-30)
Chemical denaturant titrations can be used to accurately determine protein stability. However, data acquisition is typically labour intensive, has low throughput and is difficult to automate. These factors, combined with high protein consumption, have limited the adoption of chemical denaturant
Florian Platten et al.
The journal of physical chemistry. B, 119(48), 14986-14993 (2015-11-07)
Protein phase behavior and protein-protein interactions can be tuned by additives. We experimentally determined the phase behavior of lysozyme solutions, namely, the cloud-point temperature (CPT), in the presence of two additives, sodium chloride (NaCl) and guanidine hydrochloride (GuHCl). Their concentrations
K V Greco et al.
Journal of biomaterials applications, 30(2), 239-253 (2015-04-10)
Off-the-shelf availability of tissue-engineered skin constructs, tailored by different combinations of reagents to produce a highly preserved biological matrix is often the only means to help patients suffering skin damage. This study assessed the effect of five different decellularisation methods
Venkata Ramana Katla et al.
Chemical & pharmaceutical bulletin, 61(1), 25-32 (2013-01-11)
A series of novel guanidine derivatives were synthesized in three steps and their anti-inflammatory activities in vitro and in vivo evaluated. 2-Aminopyridin-3-ol (1) was reacted with thiophosphoryl chloride (2) to give a monochloride (3). It was further reacted with cyanamide
Seoungwoo Shin et al.
Molecules (Basel, Switzerland), 20(3), 3549-3564 (2015-02-24)
Glycation is an ageing reaction of naturally occurring sugars with dermal proteins, with clinical signs appearing in vivo around age 30, and increasing steadily/regularly with age. The suppleness of the dermis is affected by the formation of bridges between proteins
Ewa M Koźma et al.
Experimental biology and medicine (Maywood, N.J.), 239(12), 1575-1588 (2014-06-11)
Tumor transformation and progression both lead to extracellular matrix remodeling, which is also reflected in an alteration in the proportion of dermatan sulphate (DS) and chondroitin sulphate (CS) and an accumulation of the latter. In addition, a significant increase in
Yuetian Yan et al.
Biochemistry, 54(34), 5322-5328 (2015-08-13)
The type 2 L-serine dehydratase from Legionella pneumophila (lpLSD) contains a [4Fe-4S](2+) cluster that acts as a Lewis acid to extract the hydroxyl group of L-serine during the dehydration reaction. Surprisingly, the crystal structure shows that all four of the
Xu-Ying Wang et al.
Small (Weinheim an der Bergstrasse, Germany), 11(43), 5826-5832 (2015-10-01)
S-layer proteins create a cell-surface layer architecture in both bacteria and archaea. Because S-layer proteins self-assemble into a native-like S-layer crystalline structure in vitro, they are attractive building blocks in nanotechnology. Here, the potential use of the S-layer protein EA1
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