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Chromogenic
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simulation results, V75 = 510 L/m2,
so this area ratio is acceptable
Step 5: Size the process
• Calculate area of NFP required
• Batch Size: 1,200 L; Batch time: 4 hours
• ANFP (m2)
(absorption) at 510 nm, Em at 590 nm.11
Ex (absorption) at 482 nm (blue-green), Em at 616 nm
(red-orange).12 The fluorescence yield of EtBr increases
as solvent polarity decreases.13
Ethidium bromide
(absorption) at 510 nm, Em at 590 nm.11
Ex (absorption) at 482 nm (blue-green), Em at 616 nm
(red-orange).12 The fluorescence yield of EtBr increases
as solvent polarity decreases.13
Ethidium bromide