quenching. For longer wavelengths, Atto 550 and
Atto 565 are efficiently excited by HeNe lasers (543 nm) and
are used as alternatives to rhodamine dyes, Cy™3, or Alexa 550,
offering more intense brightness
method 1311 for TCLP analysis
• Maintains structural integrity with-
out weight loss when ignited to
550 °C (1022 °F) after sample
filtration
• Recommended for determining
volatile suspended matter in
product can be measured using an
excitation wavelength of 450−495 nm and an emission
wavelength of 505–550 nm. In a model cell system, the
murine macrophage cell line RAW 264.7, in which iNOS
is induced
Glycoprotein Value [%]
N-acetylneuraminosyl-2,3-lactose 100
Bovine mucin 140
Fetuin 270
α1 -acid glycoprotein 550
Neuraminidase from Clostridium perfringens cleaves also N-glycolyl- as well as the 7- and 9-O-acetylsialic
phase.2 PDGFR α (T674I) is one of
the mutant forms of PDGFR α.
Recombinant human PDGFR α (T674I) (550-end) was
expressed by baculovirus in Sf9 insect cells using an
N-terminal GST-tag. The gene accession
Mix per well.
4. Incubate 20 minutes at room temperature. Read
optical density at 570 nm (550–585nm).
Note: if the Sample OD is higher than the
Standard OD at 100 g/mL, dilute sample
and vortex briefly.
3. Secure tube horizontally on a flat-bed vortex pad
with tape, or secure the tube in any commercially
available bead beater equipment (e.g.,
FastPrep®-24 Instrument). Vortex for
Bioassay Section of Plant Biotech Guide
Fluorescence Marker Kit 550
1 kit92813
E
N
8
BioChemika
For fluorescent labelling of proteins, peptides,
and amino-modified nucleotides. Comprises
yellow. Read the plate using a
spectrophotometer microplate reader set at dual wavelength of 450/550 nm (alternatively
450/540 nm or 450/595 nm may be used or a 450 nm single)
6
J. Appl.
Oryst., 24, 409-411, 1991.
2. Crystallization of nucleic acids and proteins, A. Ducruix and R. Giege eds., The Practical Approach Series, Oxford
Univ. Press, 1992.
3. Current approaches
30 min-
utes. Fluorescence was measured using a Perkin Elmer
HTS 7000 Plus BioAssay Reader set at 550 nm excitation
and 595 nm emission. Readings from blank wells (con-
taining resazurin but without cells
250 each
Z35,809-6 blue
Transmission peak. . . . . . 475 nm (no
significant transmission above 550 nm.)
not available in EU
25 each
250 each
Z35,808-8 red
Transmission peak. . 660 nm (Transmits
2 h
but it can be extended to 3 h without influencing the
results. Alternatively, the reaction can be carried out at
+2 to +8° C with a reaction time of 18 h up to 24 h.
4.7 Influence
µl final
volume each
11 585 550 910
Nylon Membranes, positively
charged
10 sheets, 20 x 30 cm 11 209 272 001
20 sheets, 10 x 15 cm 11 209 299 001
1 roll, 0.3 x 3 m 11
standard methods on the
screening host used.
3. Identify the positive clones using standard
methods.
Note: IPTG/X-gal screening is effective in the
first 24 hours post plating as the T7 promoter is
highly