75 µL
2. Add 80 µL of Working Reagent to each sample
well. Tap plate to mix.
Measurement
1. Incubate for 10 minutes at room temperature.
2. Read optical density
the MTT dye to an intense violet color
formazan with an absorption maximum at 565 nm. The
increase in absorbance at 565 nm is directly
proportional to aconitase activity.
This kit is suitable for
with MTT/NAD
that results in a colored product with an
absorption maximum at 565 nm. The
increase in absorbance at 565 nm is directly
proportional to the sorbitol concentration.
The linear detection
optical density at 565 nm for
time “zero” (OD0).
2. Incubate the plate for 15 minutes at
room temperature.
3. At the 15-minute incubation point, read the plate
again at
protein gel stains, Lucy-506, Lucy-565 and Lucy-569 are available. The profile
of each of the dyes is different: Lucy-506 shows highest sensitivity on SDS gels, Lucy-565 allows neutral
staining (e.g. for
2. Add 80 L of Blank Working Reagent to
each Sample Blank well.
3. Mix well.
4. Incubate the plate for 30 minutes at
room temperature.
5. Measure the optical density (OD) at
565
protein gel stains, Lucy-506, Lucy-565 and Lucy-569 are available. The profile of each
of the dyes is different: Lucy-506 shows highest sensitivity on SDS gels, Lucy-565 allows neutral staining (ideal e.
protein gel stains, Lucy-506, Lucy-565 and Lucy-569 are available. The profile of each
of the dyes is different: Lucy-506 shows highest sensitivity on SDS gels, Lucy-565 allows neutral staining (ideal e.
Co. or Millipore (Canada) Ltd. 2149 Winston Park,
Dr. Oakville, Ontario, L6H 6J8, Phone: +1 800-565-1400
The life science business of Merck KGaA, Darmstadt, Germany operates as
MilliporeSigma in the
Co. or Millipore (Canada) Ltd. 2149 Winston Park,
Dr. Oakville, Ontario, L6H 6J8, Phone: +1 800-565-1400
The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in
Journal of
Medicine, 84(suppl 6A), 37-42 (1988).
4. Barret, A. J., Cathepsin G. Meth. Enzymol., 80(C),
561-565 (1981).
5. Handbook of Enzyme Inhibitors, 2nd ed., Pt. A,
Zollner, H., (New York, NY: 1993