determined via a coupled enzymatic
reaction. The fluorescence intensity, measured at
λex = 535 nm/λem = 590 nm, is proportional to the
amount of methanol present in the sample.
The kit’s linear range
read in the multiwell plate reader at 405 nm with
corrections at 570 or 590 nm.
c. Subtract the readings at 590 nm from the readings
at 405 nm, to correct for optical imperfection of the
read in the multiwell plate reader at 405 nm with
corrections at 570 or 590 nm.
c. Subtract the readings at 590 nm from the readings
at 405 nm, to correct for optical imperfection of the
afferents and binding of WGA and
choleragenoid to nodose ganglion neurons in
rodents. Brain Res., 590, 207-212 (1992).
15. Hawari, F.I. et al., Release of full-length 55-kDa
TNF receptor 1 in exosome-like
available in EU
25 each
250 each
Z35,808-8 red
Transmission peak. . 660 nm (Transmits
above 590 nm)
not available in EU
. . . . . . . . . . . . . . . . . . .
contrast (DIC) and fluorescence microscopy images of Catalog No. CLL1035 (ex 530-560/em 590-650, 40×/1.4 oil).
6
References
1. Centers for Disease Control, Biosafety in
Microbiological and
Cold Spring
Harbor, NY), pp. 726 (1989).
19. Pampori, N.A. et al., BioTechniques, 18(4), 589-590 (1995).
Western Blot Troubleshooting Guide16-19
Problem Possible Cause Solution
High
a plate
reader, which does not have the exact wavelength
filter, a filter in the range of 540-590 nm can
be substituted.
2. The ratio of BCA Working Reagent to protein
sample will have to
(Cold Spring
Harbor, NY), pp. 726 (1989).
60. Pampori, N.A. et al., BioTechniques, 18(4), 589-590 (1995).
Western Blot Troubleshooting Guide56-60
Problem Possible Cause Solution
High
Background
Toxicol
Sci 83, 282-292 (2005).
47. Rogue A et al. Comparative gene expression profiles
induced by PPARγ and PPARα/γ agonists in human
hepatocytes. PLoS One 6(4), e18816 (2011).
48. Fievet C et
button when finished entering values.
6. Use the desired navigation button to exit the screen.
Cogent® µScale Tangential Flow Filtration System 47
Selecting the Units Displayed
1. On the
),
protein kinase C (Ki = 47 µM), and protein kinase G (Ki = 3.8 µM).
10 mg
Casein Kinase I Inhibitor, D4476 218696 {CkI Inhibitor; 4-(4-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-
5-pyridin-2-yl-1h-imidazol
Wee1 Inhibitor II
(6-Butyl-4-(2-chlorophenyl)-9-hydroxypyrrolo[3,4-c]carbazole-1,3-(2H,6H)-dione)
A potent, ATP-binding site-targeting inhibitor of Wee1
(IC50 = 59 nM) with a ~590-fold selectivity over
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated
P5704 5579 PRKCB M NP_002729.2 y y y
589 Protein Kinase Cη P8090 5583 PRKCH P NP_006246.2 y n/d n/d
590 Protein Kinase D P3987 5587 PRKD1 P NP_002733.2 y y n/d
420 MAP Kinase, Activated/Monophosphorylated