Z748307 Large 1 L load flask and bulb-tube, one straight tube, 24/40 joints
Z748315 X-Large 2 L load flask and bulb-tube, one straight tube, 24/40 joints
Kugelrohr Load Flasks
Robust glass rod enables
diameter
(mm)
Growth
area (cm2)
Factor to 24-
wells
Media volume
(ml)
DNA/ESCORT V
cocktail to add
100 mm 100.00 78.50 41.00 21.00 3 - 8 ml
60 mm 60.00 30.00 14.70 8.00 1 -
Gebrauchsanweisung:
1. Teststäbchen 1s mit beiden Testfeldern in Probe (pH 1–9) eintauchen.
2. Flüssigkeitsüberschuss abschütteln.
3. 60 s warten.
4. Teststäbchen mit Farbskala vergleichen. Sind Nitrat-Ionen vorhanden
steps 2-4 every day for a total of 4 days.
Day 8: Quality Control of Hindgut differentiation.
6. Set up flow analysis for CDX-2, a hindgut endoderm marker. Expression of CDX-2 must be >
Gebrauchsanweisung:
1. Teststäbchen 1s mit beiden Testfeldern in Probe (pH 1–9) eintauchen.
2. Flüssigkeitsüberschuss abschütteln.
3. 60 s warten.
4. Teststäbchen mit Farbskala vergleichen. Sind Nitrat-Ionen vorhanden
PBS 48 mL
Catalog Number MAK194A
Formalin (10%) 24 mL
Catalog Number MAK194B
Oil Red O 60 mg
Catalog Number MAK194C
Hematoxylin 24 mL
Catalog Number MAK194D
membrane panel facing up and spin at 5,000 × g maximum for
approximately 15–60 minutes.
NOTE: Refer to Figures 1 and 2, and Tables 1 and 2 for typical spin times.
4. To recover the concentrated solute, insert
sequencing products):
1. 95 °C for 5 minutes
2. 95 °C for 30 seconds
3. 50 °C for 10 seconds
4. 60 °C for 4 minutes
5. Repeat steps 2–4 for a total of 100
Boyer, ed.). Academic Press (New York
and London), pp. 562-608 (1971).
2. Jacobs, M. et al., Nucleic Acids Res., 13(24),
8913-8926 (1985).
3. Ottesen, M., and Svendsen, I., Methods Enzymol.
assay, add 24 µL to 36 µL water).
Using seven glycerol – free tubes, perform 2-fold serial dilutions from 1:2.5 – 1:160, using
Incubation Solution + 2% BSA as the diluent. For example, add 60
Working Reagent according to Table 2. Fresh
reconstitution is recommended.
Table 2.
Preparation of Working Reagent
Reagent Working Reagent
Assay Buffer 60 µL
Enzyme A 1 µL