1. Adams, J., Proteasome inhibition in cancer:
development of PS-341., Semin. Oncol. 28, 613-
619 (2001).
2. Berenson, J.R., et al., The role of nuclear factor-κΒ
in the biology and treatment
A, Taylor DL. Systems cell biology based on high-content
screening. Methods Enzymol. 2006;414:601-619. PMID: 17110213
3. Gough AH, Johnston PA. Requirements, features, and performance of high content
Nucleic Acids Res., 7, 1513-1522 (1979).
2. Vogelstein, B., and Gillespie, D., Preparative and analytical purification of DNA from
agarose. Proc. Natl. Acad. Sci. USA, 76, 615-619 (1979)
Troubleshooting
Nucleic Acids Res., 7, 1513-1522 (1979).
2. Vogelstein, B., and Gillespie, D., Preparative and analytical purification of DNA from
agarose. Proc. Natl. Acad. Sci. USA, 76, 615-619 (1979)
Troubleshooting
Nucleic Acids Res. 7, 1513-1522 (1979).
2. Vogelstein, B., and Gillespie, D., Preparative and analytical purification of DNA from agarose. Proc.
Natl. Acad. Sci. USA, 76, 615-619 (1979).
3. Ausubel
A, Taylor DL. Systems cell biology based on high-content
screening. Methods Enzymol. 2006;414:601-619. PMID: 17110213
3. Gough AH, Johnston PA.Requirements, features, and performance of high content
Acids Res., 7, 1513-1522 (1979).
8
2. Vogelstein, B., and Gillespie, D., Preparative and analytical purification of DNA from
agarose. Proc. Natl. Acad. Sci. USA, 76, 615-619 (1979).
A, Taylor DL. Systems cell biology based on high-content
screening. Methods Enzymol. 2006;414:601-619. PMID: 17110213
3. Gough AH, Johnston PA. Requirements, features, and performance of high content
Nucleic Acids Res., 7, 1513-1522 (1979).
2. Vogelstein, B., and Gillespie, D., Preparative and analytical purification of DNA from
agarose. Proc. Natl. Acad. Sci. USA, 76, 615-619 (1979).
in growth media
until ~70-80% confluent.
2. Detach cells from culture flasks/plates via method appropriate for cell type
of interest. Adjust cell density to 5-7 x 104 cells/mL in growth media
A, Taylor DL. Systems cell biology based on high-content
screening. Methods Enzymol. 2006;414:601-619. PMID: 17110213
3. Gough AH, Johnston PA.Requirements, features, and performance of high content
bbc3, C-Terminal P4618 P y y n/d
617 PUMA/bbc3, N-Terminal P4743 P y n/d n/d
618 Pyk2 P3902 P y y y
619 AP2 beta P7114 M y n/d n/d
620 phospho-Pyk2 (pTyr579/580) P6989 P y n/d n/d
621 AP2 alpha P6739 M y
n/d n/d
618 Pyk2 P3902 50646, 2185, 19229 Ptk2b,PTK2B NP_059014.2,NP_775268.1,NP_766086.1 P y y y
619 phospho-Pyk2 (pTyr579) P7114 50646, 2185 Ptk2b,PTK2B NP_059014.2,NP_775268.1 P y n/d y
620 phospho-
n/d n/d
618 Pyk2 P3902 50646, 2185, 19229 Ptk2b,PTK2B NP_059014.2,NP_775268.1,NP_766086.1 P y y y
619 phospho-Pyk2 (pTyr579) P7114 50646, 2185 Ptk2b,PTK2B NP_059014.2,NP_775268.1 P y n/d y
620 phospho-