wavelength >650 nm.
Fig. 1: Proliferation of 7TD1 cells (mouse-mouse hybridoma) in response to recombinant human interleukin-6 (hIL-6)
using the described protocol.
5
6
1
2
3
4
changes of the following
solution: 150 ml of acetic acid, 200 ml of
acetone, 650 ml of water.
References
1. Conn’s Biological Stains, Lillie, ed., p. 577 (1977).
2. Schnell S. A., et al.
Immunol Methods.,
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7.
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105(51), 20146-20151 (2008).
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(2001).
7
Koopman, M. J., Jongerius, E. (1967): Enumeration of Bacillus cereus in
foods. Appl. Microbiol. 15, 650-653.
Holbrook, R., Anderson, J. M. (1980): An im-proved selective and diagnostic medium for the
is 650 nM at this
point.
Dilution of the N-TER Peptide/siRNA nanoparticles
The concentration of the siRNA in this method is
based on a final experimental volume of 160 µL per
well.
1. Combine
15 minutes. A dual wavelength is recommended
with reference filter of 600–650 nm.
3
Results
Calculations
1. Check Calibrator Factor (CF) value on the
calibrator bottle. This value
15 minutes. A dual wavelength is recommended
with reference filter of 600–650 nm.
3
Results
Calculations
1. Check Calibrator Factor (CF) value on the calibrator
bottle. This value
Koopman, M.J. and Jongerius, E. (1967): Enumeration of Bacillus cereus in
foods. Appl. Microbiol. 15: 650-653.
GranuCult, Merck, Millipore, and Sigma-Aldrich are trademarks of Merck KGaA,
Darmstadt
flow
cytometry.
� Registered Trademark of Amersham Biosciences Limited
Fluorescence Marker Kit 650
1 kit92821
E
N
8
BioChemika
For fluorescent labelling of proteins, peptides,
and amino-modified
concentration of the siRNA in the Nanoparticle Formation Solution prior to dilution in culture
medium is 650 nM.
Preparation of the Nanoparticle Formation Solutions
Unlike most lipid-based transfection reagents
in colorectal cancers. Gut, 51, 56-59 (2002).
3. Papageorgis, P. et al., Smad4 inactivation
promotes malignancy and drug resistance of colon
cancer. Cancer Res., 71(3), 1-11 (2011).
4. Hahn, S.A.
Springer. (1987). Purified intercellular adhesion molecule-1
(ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell
51: 813-819.
3. Dustin, M.L., R. Rothlein, A.K. Bhan, C.
Nervous System. Pharmacol. Reviews,
51, 439-464 (1999).
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and storage in synaptic vesicles. Jpn. J.
Pharmacol., 77, 1-10 (1998).
5. Pyle, R.A. et al
c-terminus.
04-1062 Rabbit This antibody recognizes GFAP at and around the n-terminus.
07-650 Rabbit GFAP+1
Gross cystic
disease
fluid protein
(GCDFP-15)
Breast cancer, ovarian cancer, salivary
References 1 Löbner, K. et al. (2002) Different Regulated Expression of the Tyrosine
Phosphatase-Like Proteins IA-2 and Phogrin by Glucose and Insulin in
Pancreatic Islets Diabetes 51, 2982-2988.
abundant in
human blood: characterization of a radioimmunoassay recognizing PYY 1-36
and PYY 3-36. Regulatory Peptides. 51(1994):151-9, 1994.
4. Westgard, J.O., et. al. A multi-rule Shewhart chart for
abundant in human blood: characterization of a
radioimmunoassay recognizing PYY 1-36 and PYY 3-36. Regulatory Peptides. 51(1994):151-9,
1994.
4. Westgard, J.O., et. al. A multi-rule Shewhart chart