653152

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Screening ubiquitin specific protease activities using chemically synthesized ubiquitin and ubiquitinated peptides.

Marine Bacchi et al.

Analytical biochemistry, 519, 57-70 (2016-12-21)

Ubiquitin, a 76 amino acid protein, is a key component that contributes to cellular protein homeostasis. The specificity of this modification is due to a series of enzymes: ligases, attaching the ubiquitin to a lysine, and deubiquitinases, which remove it.

Direct on-resin synthesis of peptide-αthiophenylesters for use in native chemical ligation

Bang D, et al.

Organic Letters, 8(6), 1049-1052 (2006)

Structure of a human 48S translational initiation complex.

Jailson Brito Querido et al.

Science (New York, N.Y.), 369(6508), 1220-1227 (2020-09-05)

A key step in translational initiation is the recruitment of the 43S preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48S initiation complex (i.e., the 48S).

The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members.

Amrita Rai et al.

Nature communications, 11(1), 4187-4187 (2020-08-23)

EHBP1 is an adaptor protein that regulates vesicular trafficking by recruiting Rab8 family members and Eps15-homology domain-containing proteins 1/2 (EHD1/2). It also links endosomes to the actin cytoskeleton. However, the underlying molecular mechanism of activation of EHBP1 actin-binding activity is

Modular Synthesis of Trifunctional Peptide-oligonucleotide Conjugates via Native Chemical Ligation.

Mohadeseh Dastpeyman et al.

Frontiers in chemistry, 9, 627329-627329 (2021-03-20)

Cell penetrating peptides (CPPs) are being increasingly used as efficient vectors for intracellular delivery of biologically active agents, such as therapeutic antisense oligonucleotides (ASOs). Unfortunately, ASOs have poor cell membrane permeability. The conjugation of ASOs to CPPs have been shown

Metal Exchange in the Interprotein ZnII -Binding Site of the Rad50 Hook Domain: Structural Insights into CdII -Induced DNA-Repair Inhibition.

Michał Padjasek et al.

Chemistry (Weinheim an der Bergstrasse, Germany), 26(15), 3297-3313 (2019-12-18)

CdII is a major genotoxic agent that readily displaces ZnII in a multitude of zinc proteins, abrogates redox homeostasis, and deregulates cellular metalloproteome. To date, this displacement has been described mostly for cysteine(Cys)-rich intraprotein binding sites in certain zinc finger

Efficient palladium-assisted one-pot deprotection of (acetamidomethyl) cysteine following native chemical ligation and/or desulfurization to expedite chemical protein synthesis

Maity SK, et al.

Angewandte Chemie (International Edition in English), 55(28), 8108-8112 (2016)

Cyclisation increases the stability of the sea anemone peptide APETx2 but decreases its activity at acid-sensing ion channel 3.

Jonas E Jensen et al.

Marine drugs, 10(7), 1511-1527 (2012-08-02)

APETx2 is a peptide isolated from the sea anemone Anthopleura elegantissima. It is the most potent and selective inhibitor of acid-sensing ion channel 3 (ASIC3) and it is currently in preclinical studies as a novel analgesic for the treatment of

Flexible antibodies with nonprotein hinges.

Daniel J Capon et al.

Proceedings of the Japan Academy. Series B, Physical and biological sciences, 87(9), 603-616 (2011-11-15)

There is a significant need for antibodies that can bind targets with greater affinity. Here we describe a novel strategy employing chemical semisynthesis to produce symmetroadhesins: antibody-like molecules having nonprotein hinge regions that are more flexible and extendible and are

Ultrasensitive immunosensor for acrylamide based on chitosan/SnO2-SiC hollow sphere nanochains/gold nanomaterial as signal amplification.

Min-Fu Wu et al.

Analytica chimica acta, 1049, 188-195 (2019-01-08)

An electrochemical immunosensor for ultrasensitive detection of acrylamide (AA) in water and food samples was developed. SnO2-SiC hollow sphere nanochains with high surface area and gold nanoparticles with good electroconductivity were fabricated onto the surface of a glassy carbon electrode

Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations.

Vangelis Agouridas et al.

Chemical reviews, 119(12), 7328-7443 (2019-05-06)

The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester with an N-terminal cysteinyl peptide to produce a native peptide bond between the two fragments. This reaction has considerably extended the size of polypeptides and proteins that can

Chemical synthesis of a polypeptide backbone derived from the primary sequence of the cancer protein NY-ESO-1 enabled by kinetically controlled ligation and pseudoprolines.

Paul W R Harris et al.

Biopolymers, 104(2), 116-127 (2015-02-07)

The cancer protein NY-ESO-1 has been shown to be one of the most promising vaccine candidates although little is known about its cellular function. Using a chemical protein strategy, the 180 amino acid polypeptide, tagged with an arginine solubilizing tail

Backbone Cyclization and Dimerization of LL-37-Derived Peptides Enhance Antimicrobial Activity and Proteolytic Stability.

Sunithi Gunasekera et al.

Frontiers in microbiology, 11, 168-168 (2020-03-11)

Can antimicrobial activity and peptide stability of alpha-helical peptides be increased by making them into dimers and macrocycles? Here, we explore that concept by using KR-12 as the starting point for peptide engineering. KR-12 has previously been determined as the

Palladium mediated rapid deprotection of N-terminal cysteine under native chemical ligation conditions for the efficient preparation of synthetically challenging proteins

Jbara M, et al.

Journal of the American Chemical Society, 138(15), 5069-5075 (2016)

Making Ends Meet: Microwave-Accelerated Synthesis of Cyclic and Disulfide Rich Proteins Via In Situ Thioesterification and Native Chemical Ligation.

Sunithi Gunasekera et al.

International journal of peptide research and therapeutics, 19(1), 43-54 (2013-03-19)

The development of synthetic methodologies for cyclic peptides is driven by the discovery of cyclic peptide drug scaffolds such as the plant-derived cyclotides, sunflower trypsin inhibitor 1 (SFTI-1) and the development of cyclized conotoxins. Currently, the native chemical ligation reaction

Imidazole-Aided Native Chemical Ligation: Imidazole as a One-Pot Desulfurization-Amenable Non-Thiol-Type Alternative to 4-Mercaptophenylacetic Acid

K Sakamoto, et al.

Chemistry?A European Journal , 22, 17940-17944 (2016)

A method for the unbiased and efficient segmental labelling of RNA-binding proteins for structure and biophysics.

Christopher Gallagher et al.

Scientific reports, 7(1), 14083-14083 (2017-10-28)

Most eukaryotic RNA regulators recognise their RNA and protein partners by the combinatorial use of several RNA binding domains. Inter-domain dynamics and interactions play a key role in recognition and can be analysed by techniques such as NMR or FRET

Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) for targeted antibody blocking.

Lin Zhang et al.

Chemical science, 10(11), 3271-3280 (2019-04-19)

Targeted antibody blocking enables characterization of binding sites on immunoglobulin G (IgG), and can efficiently eliminate harmful antibodies from organisms. In this report, we present a novel peptide-denoted as a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF)-for targeted

One-pot chemical synthesis of small ubiquitin-like modifier protein-peptide conjugates using bis (2-sulfanylethyl) amido peptide latent thioester surrogates

Boll E, et al.

Nature Protocols, 10(2), 269-269 (2015)

Mirror-image polymerase chain reaction.

Wenjun Jiang et al.

Cell discovery, 3, 17037-17037 (2017-10-21)

The construction of mirror-image biological systems may open the next frontier for biomedical technology development and discovery. Here we have designed and chemically synthesized a mutant version of the thermostable

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