Ki-67
The Ki-67 antigen (Ki-67) is a classic marker of cellular proliferation and has
found widespread application in diagnostic, research and drug discovery
applications [9-11]. The Ki-67 antigen
exists as two isoforms, GAD65 and GAD67
(molecular masses of 65 and 67 kDa, respectively) that
are encoded by two different genes.
4, 9-10
GAD65 is an ampiphilic, membrane-anchored protein,
(585
a concentration1
between mouse and human IL-9. Overall sequence not less than 1 )g/ml.1
homology between human and mouse IL-9 cDNA's is 56%
and 67% identity at the amino acid and nucleotide levels
avidity" for biotin.
Avidin is a tetrameric glycoprotein with an approximate
molecular mass of 66–67 kDa.
5
It is composed of four
subunits with each subunit containing 128 identical
amino
Citron, M., et al., Nature Med., 3, 67-72 (1997).
7. Kim, T-W. and Tanzt, R. E., Curr. Opin. Neurobiol.,
7, 683-688 (1997).
8. Haass, C., Neuron, 18, 687-690 (1997).
9. Levitan, D., et al., Proc. Natl.
= 4.
Figure 5. Multiparameter analysis of phospho-
histone H3 and Ki-67. Upper Panels. Phospho-
histone H3 and Ki-67 dose responses of A549 and
HeLa cells to cell cycle-arresting agents. Lower
0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼67 kDa
Purity: 80–95% (SDS-PAGE, see Figure 1)
Specific Activity: 67–91 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
at −20°C
References
1. Franek, F., et al., Effect of the Purine Derivative
Myoseverin and its analogues on cultured
hybridoma Cells. Collection of Czechoslovak
Chemical Communications, 67(2)
lower than
those required for ∆9-THC.4
Preparation Instructions
HU-210 is soluble in DMSO at 20 mg/ml. It is insoluble
in water.
Storage/Stability
Store at –20 °C under nitrogen, desiccated.
deletion 9/deletion 9/deletion 36/
deletion 36
Figure 2.
Creation of BCRP Knockout in C2BBe1 Cells
Site-specific deletion at the BCRP Locus in C2BBe1
cell line.
Alleles 1 and 2 – 67 bp deletion
dissertation, p. 67
(September 2018).
44. Buck, Chelsea, "Characterization and
functionalization of suckerin-12 protein
hydrogels". University of Dayton, Ph.D.
dissertation, pp. 41, 67, 99 (December
Characterization of
heme acquisition in Leishmania”. University of
Maryland College Park, Ph.D. dissertation, p. 67
(2014).
13. Bhatia, Sanil, “A role of nucleolin
in human hematopoietic progenitor cells”.
Citron, M., et al., Nature Med., 3, 67-72 (1997).
7. Kim, T-W. and Tanzt, R. E., Curr. Opin. Neurobiol.,
7, 683-688 (1997).
8. Haass, C., Neuron, 18, 687-690 (1997).
9. Levitan, D., et al., Proc. Natl
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼67 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 67–91 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼67 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 67–91 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼67 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 67–91 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼67 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 67–91 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This
Sambrooke and Tooze
Publication at Oxford University Press, 1994),
pp. 67-69.
2. Oppenheim, J et al., Annu. Rev. Immunol., 9,
617-648, (1991).
3. Shröeder, J. et al., J. Immunol., 139, 3474
Made Solution
from Streptomyces hygroscopicus
Catalog Number R8781
Storage Temperature –20 °C
CAS RN 53123-88-9
Synonyms: Antibiotic AY 22989; NSC 2260804;
Sirolimus; Rapamune.
Product Description