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Casting Equilibrating and Seeding the 3D Petri Dish
µl
12-60TR Troughs 60 4 x 15 500 µl 190 µl
12-36TO Toroids 36 6 x 6 500 µl 190 µl
24-96 Small spheroids 96 8 x 12 330 µl 75 µl
24-35 Larger
The 3D Petri Dish
seeded
(cells/75 l)
50 15 3,840/190 l 1,440/75 l
100 125 32 000/190 l 12 000/75 l100 125 32,000/190 l 12,000/75 l
150 421 107,000/190 l 40,000/75 l
200 1,000 256,000/190 l 96,000/75 l
250 1,953
Calcium carbonate as a replacement for titanium dioxide in coating
43 5
100
105
95
90
85
80
75
70
65
60
55
50
45
40
35
30
25
20
15
10
5
0
b* L*a*
a*
a
nd
b
*
L*
PVA HPMC PVA HPMC
PVA HPMC PVA HPMC PVA HPMC
4% Polymer
20% Plasticizer
30% CaCO3
Data Sheet - A3356
immobilized on agarose.
Anti-β-Amyloid (22-35) specifically recognizes β-amyloid
(22-35), and β-amyloid (1-40). The antibody detects
β-amyloid (1-40) by immunoblotting (~4 kDa). Staining
of the β-amyloid (1-
The Vmax Nomograph
0.
00
00
75
0.
00
00
5
0.
00
00
35
0.
00
06
0.
00
10
0.
00
25
0.
00
35
0.
00
75
0.
01
5
=
in m2/L
0.000035
A comparative study of hydrophobic and hydrophilic lubricants for optimal combination of galenical and dissolution properties
itraconazole.
Dissolution performace of itraconazole
1000 mL SGFsp pH 1.2; 75 rpm
Time [min]
5
1
2
3
4
0
0 15 30 45 60 75 90 105 120
Pure itraconazole Tablets with
Analysis of Cannabinoids Using Ascentis C18
.D., 3 µm particles (581321-U)
mobile phase: 25:75, 10 mM ammonium acetate (pH 4.5 with acetic
acid):acetonitrile
flow rate: 1 mL/min.
temp.: 35 °C
det.: UV at 220 nm
injection: 10 µL
sample
Enhancing the Solubility of Active Pharmaceutical Ingredients Using Hot Melt Extrusion and Polyvinyl Alcohol
manufacture using
VCM and after storage (2, 4 weeks at 40 °C/75% rH) in comparison to
the physical mixture of both and pure crystalline API.
5 10 15 25 30 35
°2 Theta
IndomethacinC
20
Product Information Sheet - M9445
as a 0.2 µm filtered solution of
25 mM Tris, pH 7.5, with 5 mM calcium chloride,
75 mM sodium chloride, 0.025% BRIJ 35, and 50%
glycerol.
MMP-2 may be used to study
Data Sheet - I2276
Factor (NKSF) is a disulfide-linked heterodimer of a
35 kD light chain subunit and 40 kD heavy chain
subunit. The molecular weight of IL-12 is approximately
75 kD. The p35 subunit of IL-12 shares amino acid
User Guide - Steritest Standard Devices
50
75
30 75
TZHAPC210 75
TZHACA210 75
TZHALV2xx 150
TZHASV2xx 75
Red canister
base
TZHVAB2xx 75
TZHVCA210 75
TZHVLV2xx 150
TZHVSV2xx 75
Green
canister base TZHVSL210 75
Do not
Data Sheet - I1270
containing 0.1% HSA or BSA to a concen-
35 kD light chain subunit and 40 kD heavy chain subunit. tration not less than 1 µg/ml.
The molecular weight of IL-12 is approximately 75 kD.
The p35 subunit of IL-12 shares
Data Sheet - A4718
size by
only 2% can be resolved.
Physical Properties
Gelling temperature (3%) ≤35°C
Melting temperature (3%) ≤75°C
Gel strength (3%) ≥300 g/cm2
Precautions and Disclaimer
Sigma’s High Resolution
Product Information Sheet - A5479
Desired Final Ammonium Sulfate Percent Saturation
10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90
Product Information Sheet - D5628
150 ml of absolute ethanol at
75 ºC. Digitonin is precipitated by chilling the solution
at 0 ºC (ice water) for 20 minutes and then separated
by centrifugation at 4 ºC. This procedure is repeated
FcFusionProteinSeparation
Biosimilar (1-3 mg/mL)
min0 2 4 6 8 10 12 14 16 18
mAU
0
25
50
75
100
125
150
175
8.
44
8
9.
73
0
Data Sheet - N7404
determinations are best made following 16-18 hours incubation
at 35-37°C. Acidimetric determinations are best made following 72 hours incubation at 35-37°C.
The vibrant M, Millipore
Pharmacopoeia Monograph Methods
1500
22
4
35
2
25
1
9.00 9.25 9.50 9.75 min
0.00
0.25
0.50
0
250
500
Zero Line
UV spectra: Z-isomer Peak purity curve: Z-isomer
1000
1250
User Guide - SAE0176
Circ.
Physiol. 303: H919–H930 (2012).
4. Page-McCaw, A. et al., Nat. Rev. Mol. Cell. Biol.
8: 221–233 (2007).
5. Hannocks, M.J. et al., Matrix. Biol. 75–76:
102-113 (2019).
6. Medeiros. N.
Data Sheet - P6851
after 18-24 hours at 35-37°C.
Organisms (ATCC) Growth
Lactobacillus plantarum (8014) Increase in growth with increasing concentrations of calcium
pantothenate (0, 25, 50, 75, 125, 150, 200, 250
The Effective Use of Protein Kinase Inhibitors
60 99 91 95 96 73 77 99
SGK 25 109 92 81 78 91 111 90 83 98 101 72 35 108 99 100 21 63 26
S6K1 0 94 32 98 93 92 86 100 87
HLA003 - Technical Bulletin
cells
Red: A549 HLA-B*08:01 cells
HLA003J-
1VL:
A549
HLA-
B*35:01
Cells
Blue: A549 B2M KO cells
Red: A549 HLA-B*35:01 cells
Fast GC-A Practical Guide for Increasing Sample Throughput without Sacrificing Quality
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5758
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Data Sheet - T4930
factor
(SCF), IL-3, IL-6, and IL-11.
TPO is an ~35 kDa polypeptide of 335 amino acids.
However, due to glycosylation, the protein has an
apparent MW of 75 kDa in SDS-PAGE. The precursor
form of TPO consists
Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells (AGS-NSC)
counted in 4 quadrants = 140
Total non-viable cells counted in 4 quadrants = 10
Total cells counted =150, Percent Viability = 140 viable ÷ 150 total x 100 = 93.3%
Average cells per quadrant = 35
35 cells
141464 Parteck MXP
was assessed under three different
conditions: cold (2 – 4 °C), room temperature
(25 °C, 60 % relative humidity (rH)), and
accelerated (40 °C, 75 % rH). At each time
point, the extrudates were measured
Data Sheet -17117
Examination of Water and Wastewater, 21st Ed., APHA, Washington, D.C.
4. Lechevalier H. A., 1975, Environ. Protection Technol. Ser., EPA-600/ 2-75-031, U. S.
Environmental Protection Agency, Cincinnati, Ohio
Ascentis Express F5 HPLC Columns
concentrated formic acid
mobile phase B: acetonitrile
mobile phase ratio: A:B 75:25, v/v
flow rate: 0.8 mL/min.
temp.: 35 ºC
pressure: 1635 psi
det.: UV PDA at 230 nm and MS in ESI (+), SIR (single
Mobius® and UltraMobius
...........................................3
35-ml culture (high-copy or low-copy number plasmid) 3
Culture growth 3
Preparation of cleared lysate 4
Mobius 200 Column chromatography 5
Precipitation
Anti-Fodrin, alpha, clone AA6 - Data Sheet
J. (1993) 25:678-686.
3. Lab Invest. (1993) 68:652-662.
4. Am. J. Resp. Cell Mol. Biol. (1994) 11:75-84.
5. Differentiation (1993) 54:35-45.
6. J. Bio. Chem. (1995). 270:6425-6428
7. Amer. J. Physiol
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