, 208(1), 74-79 (1993).
3. John, R. A., in Photometric assays. Enzyme
Assays A Practical Approach, Eisenthal, R., and
Danson, M. J., eds., IRL Press (New York, NY:
1992), pp. 59-92.
4. Jacks
least 10 isoforms of B-Raf have been
identified in adult mouse tissues in the ranges of 69-72
kD and 79-99 kD.1 B-Raf is highly expressed in brain
tissues2 and is activated by Ras3,4,. The 93-95 kD
isoforms
Anti-Sprouty-2 (N-Terminal) is developed in rabbit using
a synthetic peptide corresponding to amino acids 79-98
of mouse Sprouty 2 (Spry 2), conjugated to KLH via a
C-terminal added lysine residue as immunogen
1028-1032
(1999).
5. Korenbaum, E., et al., Genomic organization and expression profile of the
parvin family of focal adhesion protens in mice and humans., Gene, 279,
69-79 (2001).
N
ew
P
mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼79 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 78–106 nmole/min/mg (see Figure
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
Biochemical
Research, 3rd edition. Clarendon Press
(Oxford, UK), pp. 78-79 (1986).
28. Wilding, T.J. et al., J. Neurosci., 15(5),
4124-4132 (1995).
29. Major, T. et al., Curr. Protoc.
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
with 150 mM NaCl, 0.2 5 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see
glycerol.
Molecular mass: ∼100 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 79–107 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not
Molecular and cellular
pathogenesis of hemoglobin SC disease. Proc.
Natl. Acad. Sci. USA, 79, 7527-31 (1982).
5. Clark, M.R., et al., Study on the dehydrating effect
of the red cell Na+/K+-pump in nystatin-treated