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Showing 1-30 of 39 results for "790404P" within Papers
Jonghoon Choi et al.
International journal of nanomedicine, 9, 5189-5201 (2014-11-28)
We present here the in vitro release profiles of either fluorescently labeled biomolecules or computed tomography contrast nanoagents from engineered collagen hydrogels under physiological conditions. The collagen constructs were designed as potential biocompatible inserts into wounded human gingiva. The collagen
Yunlong Zhao et al.
Immunity, 51(6), 1059-1073 (2019-11-24)
Combined immunotherapy targeting the immune checkpoint receptors cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death 1 (PD-1), or CTLA-4 and the PD-1 ligand (PD-L1) exhibits superior anti-tumor responses compared with single-agent therapy. Here, we examined the molecular basis for
Shuai Shao et al.
Nature chemistry, 7(5), 438-446 (2015-04-23)
Methods to attach polypeptides to lipid bilayers are often indirect and ineffective, and can represent a substantial bottleneck in the formation of functionalized lipid-based materials. Although the polyhistidine tag (his-tag) has been transformative in its simplicity and efficacy in binding
Darren J Wight et al.
PLoS pathogens, 10(10), e1004474-e1004474 (2014-10-31)
The murine leukaemia virus (MLV) gag gene encodes a small protein called p12 that is essential for the early steps of viral replication. The N- and C-terminal regions of p12 are sequentially acting domains, both required for p12 function. Defects
N Bischler et al.
Biophysical journal, 74(3), 1522-1532 (1998-03-25)
Nickel-chelating lipid monolayers were used to generate two-dimensional crystals from yeast RNA polymerase I that was histidine-tagged on one of its subunits. The interaction of the enzyme with the spread lipid layers was found to be imidazole dependent, and the
Jenny J Lin et al.
Biophysical journal, 118(12), 2879-2893 (2020-05-15)
The natural peptide-major histocompatibility complex (pMHC) ligand for T cell receptors (TCRs) is inactive from solution yet capable of activating T cells at single-molecule levels when membrane-associated. This distinctive feature stems from the mechanism of TCR activation, which is thought to involve
Kathleen E Orrell et al.
Nature communications, 11(1), 432-432 (2020-01-25)
Large Clostridial Toxins (LCTs) are a family of six homologous protein toxins that are implicated in severe disease. LCTs infiltrate host cells using a translocation domain (LCT-T) that contains both cell-surface receptor binding sites and a membrane translocation apparatus. Despite
Shen Wang et al.
Protein science : a publication of the Protein Society, 29(6), 1511-1523 (2020-04-12)
Atg3-catalyzed transferring of Atg8 to phosphatidylethanolamine (PE) in the phagophore membrane is essential for autophagy. Previous studies have demonstrated that this process requires Atg3 to interact with the phagophore membrane via its N-terminal amphipathic helix. In this study, by using
Hana Petroková et al.
Pharmaceutics, 11(12) (2019-12-08)
Development of tools for direct thrombus imaging represents a key step for diagnosis and treatment of stroke. Nanoliposomal carriers of contrast agents and thrombolytics can be functionalized to target blood thrombi by small protein binders with selectivity for fibrin domains
Kelly A Dryden et al.
Protein science : a publication of the Protein Society, 18(12), 2629-2635 (2009-10-22)
Bacterial microcompartments (BMCs) are large intracellular bodies that serve as simple organelles in many bacteria. They are proteinaceous structures composed of key enzymes encapsulated by a polyhedral protein shell. In previous studies, the organization of these large shells has been
Samuel A Merrill et al.
The Journal of biological chemistry, 285(46), 35428-35438 (2010-09-02)
VPS4 proteins are AAA(+) ATPases required to form multivesicular bodies, release viral particles, and complete cytokinesis. They act by disassembling ESCRT-III heteropolymers during or after their proposed function in membrane scission. Here we show that purified human VPS4A is essentially
David J Montefusco et al.
Methods in enzymology, 423, 267-298 (2007-07-05)
The reconstitution of membrane-associated protein complexes poses significant experimental challenges. The core signaling complex in the bacterial chemotaxis system is an illustrative example: The soluble cytoplasmic signaling proteins CheW and CheA bind to heterogeneous clusters of transmembrane receptor proteins, resulting
C Dietrich et al.
Biochemistry, 35(4), 1100-1105 (1996-01-30)
The coupling of a DNA-binding protein to self-organized lipid monolayers is examined at the air-water interface by means of film balance techniques and epifluorescence microscopy. We used two recombinant species of the heat shock factor HSF24 which differ only in
Priyanka D Abeyrathne et al.
Methods in enzymology, 481, 25-43 (2010-10-05)
Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close
E Barklis et al.
The Journal of biological chemistry, 273(13), 7177-7180 (1998-04-29)
In an in vitro system that mimics the assembly of immature human immunodeficiency virus (HIV) particles, ordered arrays of HIV-1 capsid (CA) proteins encoded by the viral gag gene have been obtained by incubation of histidine-tagged capsid proteins (His-HIVCA) beneath
Diego De Miguel et al.
Cancers, 11(12) (2019-12-11)
Although TRAIL (TNF-related apoptosis-inducing ligand, also known as Apo2L) was described as capable of inducing apoptosis in transformed cells while sparing normal cells, limited results obtained in clinical trials has limited its use as an anti-tumor agent. Consequently, novel TRAIL
Xuewu Zhang et al.
Cell, 125(6), 1137-1149 (2006-06-17)
The mechanism by which the epidermal growth factor receptor (EGFR) is activated upon dimerization has eluded definition. We find that the EGFR kinase domain can be activated by increasing its local concentration or by mutating a leucine (L834R) in the
Hao Zhang et al.
Molecular & cellular proteomics : MCP, 10(6), M110-M110 (2011-03-23)
Her4 is a transmembrane receptor tyrosine kinase belonging to the ErbB-EGFR family. It plays a vital role in the cardiovascular and nervous systems, and mutations in Her4 have been found in melanoma and lung cancer. The kinase domain of Her4
Xiaozheng Xu et al.
The Journal of cell biology, 219(6) (2020-05-22)
Blockade antibodies of the immunoinhibitory receptor PD-1 can stimulate the anti-tumor activity of T cells, but clinical benefit is limited to a fraction of patients. Evidence suggests that BTLA, a receptor structurally related to PD-1, may contribute to resistance to
Yasunori Saheki et al.
Nature cell biology, 18(5), 504-515 (2016-04-12)
Acute metabolic changes in plasma membrane (PM) lipids, such as those mediating signalling reactions, are rapidly compensated by homeostatic responses whose molecular basis is poorly understood. Here we show that the extended synaptotagmins (E-Syts), endoplasmic reticulum (ER) proteins that function
E Barklis et al.
The EMBO journal, 16(6), 1199-1213 (1997-03-17)
We have developed a system for analysis of histidine-tagged (His-tagged) retrovirus core (Gag) proteins, assembled in vitro on lipid monolayers consisting of egg phosphatidylcholine (PC) plus the novel lipid DHGN. DHGN was shown to chelate nickel by atomic absorption spectrometry
John Monsey et al.
The Journal of biological chemistry, 285(10), 7035-7044 (2009-12-22)
Her4 (ErbB-4) and Her2/neu (ErbB-2) are receptor-tyrosine kinases belonging to the epidermal growth factor receptor (EGFR) family. Crystal structures of EGFR and Her4 kinase domains demonstrate kinase dimerization and activation through an allosteric mechanism. The kinase domains form an asymmetric
Scott D Hansen et al.
eLife, 4 (2015-08-22)
Enabled/Vasodilator (Ena/VASP) proteins promote actin filament assembly at multiple locations, including: leading edge membranes, focal adhesions, and the surface of intracellular pathogens. One important Ena/VASP regulator is the mig-10/Lamellipodin/RIAM family of adaptors that promote lamellipod formation in fibroblasts and drive
Elizabeth M Wilson-Kubalek et al.
Methods in enzymology, 481, 45-62 (2010-10-05)
Helical protein arrays offer unique advantages for structure determination by cryo-electron microscopy (cryo-EM). A single image of such an array contains a complete range of equally spaced molecular views of the underlying protein subunits, which allows a low-resolution, isotropic three-dimensional
C Vénien-Bryan et al.
Journal of molecular biology, 274(5), 687-692 (1998-02-07)
Two-dimensional crystals of the histidine-tagged-HupR protein, a transcriptional regulator from the photosynthetic bacterium Rhodobacter capsulatus, were obtained upon specific interaction with a Ni2+-chelated lipid monolayer. HupR is a response regulator of the NtrC family; it activates the transcription of the
Phuong A Nguyen et al.
Methods in cell biology, 128, 223-241 (2015-05-23)
Cell division in prokaryotes and eukaryotes is commonly initiated by the well-controlled binding of proteins to the cytoplasmic side of the cell membrane. However, a precise characterization of the spatiotemporal dynamics of membrane-bound proteins is often difficult to achieve in
E M Wilson-Kubalek et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(14), 8040-8045 (1998-07-08)
A general approach for crystallization of proteins in a fast and simple manner would be of immense interest to biologists studying protein structure-function relationships. Here, we describe a method that we have developed for promoting the formation of helical arrays
M J Rodrigues-Jesus et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 50(1), 67-77 (2019-01-15)
Microlipid vesicles (MLV) have a broad spectrum of applications for the delivery of molecules, ranging from chemical compounds to proteins, in both in vitro and in vivo conditions. In the present study, we developed a new set of nanosize multilayer
Katharina Dürre et al.
Soft matter, 15(47), 9676-9681 (2019-10-31)
Recently, continuous droplet interface crossing encapsulation (cDICE) was developed, which allows fast and efficient production of giant unilamellar vesicles (GUVs) under high salt conditions, at low temperature and with low consumption of the encapsulated proteins. Unfortunately, cholesterol encapsulation within the
E W Kubalek et al.
Journal of structural biology, 113(2), 117-123 (1994-09-01)
Recombinant proteins containing a short stretch of contiguous histidine residues (approximately 6) ("a His-tag") can be specifically bound to N-nitrilotriacetic-acid-chelated nickel ions, providing a convenient general method for their purification. A lipid derivatized with a nickel-chelating head group may provide
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