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Showing 1-30 of 39 results for "790404P" within Papers
C Dietrich et al.
Biochemistry, 35(4), 1100-1105 (1996-01-30)
The coupling of a DNA-binding protein to self-organized lipid monolayers is examined at the air-water interface by means of film balance techniques and epifluorescence microscopy. We used two recombinant species of the heat shock factor HSF24 which differ only in
E Barklis et al.
The Journal of biological chemistry, 273(13), 7177-7180 (1998-04-29)
In an in vitro system that mimics the assembly of immature human immunodeficiency virus (HIV) particles, ordered arrays of HIV-1 capsid (CA) proteins encoded by the viral gag gene have been obtained by incubation of histidine-tagged capsid proteins (His-HIVCA) beneath
Priyanka D Abeyrathne et al.
Methods in enzymology, 481, 25-43 (2010-10-05)
Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close
Shen Wang et al.
Protein science : a publication of the Protein Society, 29(6), 1511-1523 (2020-04-12)
Atg3-catalyzed transferring of Atg8 to phosphatidylethanolamine (PE) in the phagophore membrane is essential for autophagy. Previous studies have demonstrated that this process requires Atg3 to interact with the phagophore membrane via its N-terminal amphipathic helix. In this study, by using
Kelly A Dryden et al.
Protein science : a publication of the Protein Society, 18(12), 2629-2635 (2009-10-22)
Bacterial microcompartments (BMCs) are large intracellular bodies that serve as simple organelles in many bacteria. They are proteinaceous structures composed of key enzymes encapsulated by a polyhedral protein shell. In previous studies, the organization of these large shells has been
Hana Petroková et al.
Pharmaceutics, 11(12) (2019-12-08)
Development of tools for direct thrombus imaging represents a key step for diagnosis and treatment of stroke. Nanoliposomal carriers of contrast agents and thrombolytics can be functionalized to target blood thrombi by small protein binders with selectivity for fibrin domains
David J Montefusco et al.
Methods in enzymology, 423, 267-298 (2007-07-05)
The reconstitution of membrane-associated protein complexes poses significant experimental challenges. The core signaling complex in the bacterial chemotaxis system is an illustrative example: The soluble cytoplasmic signaling proteins CheW and CheA bind to heterogeneous clusters of transmembrane receptor proteins, resulting
Samuel A Merrill et al.
The Journal of biological chemistry, 285(46), 35428-35438 (2010-09-02)
VPS4 proteins are AAA(+) ATPases required to form multivesicular bodies, release viral particles, and complete cytokinesis. They act by disassembling ESCRT-III heteropolymers during or after their proposed function in membrane scission. Here we show that purified human VPS4A is essentially
Darren J Wight et al.
PLoS pathogens, 10(10), e1004474-e1004474 (2014-10-31)
The murine leukaemia virus (MLV) gag gene encodes a small protein called p12 that is essential for the early steps of viral replication. The N- and C-terminal regions of p12 are sequentially acting domains, both required for p12 function. Defects
Jenny J Lin et al.
Biophysical journal, 118(12), 2879-2893 (2020-05-15)
The natural peptide-major histocompatibility complex (pMHC) ligand for T cell receptors (TCRs) is inactive from solution yet capable of activating T cells at single-molecule levels when membrane-associated. This distinctive feature stems from the mechanism of TCR activation, which is thought to involve
Jonghoon Choi et al.
International journal of nanomedicine, 9, 5189-5201 (2014-11-28)
We present here the in vitro release profiles of either fluorescently labeled biomolecules or computed tomography contrast nanoagents from engineered collagen hydrogels under physiological conditions. The collagen constructs were designed as potential biocompatible inserts into wounded human gingiva. The collagen
Shuai Shao et al.
Nature chemistry, 7(5), 438-446 (2015-04-23)
Methods to attach polypeptides to lipid bilayers are often indirect and ineffective, and can represent a substantial bottleneck in the formation of functionalized lipid-based materials. Although the polyhistidine tag (his-tag) has been transformative in its simplicity and efficacy in binding
N Bischler et al.
Biophysical journal, 74(3), 1522-1532 (1998-03-25)
Nickel-chelating lipid monolayers were used to generate two-dimensional crystals from yeast RNA polymerase I that was histidine-tagged on one of its subunits. The interaction of the enzyme with the spread lipid layers was found to be imidazole dependent, and the
Yunlong Zhao et al.
Immunity, 51(6), 1059-1073 (2019-11-24)
Combined immunotherapy targeting the immune checkpoint receptors cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death 1 (PD-1), or CTLA-4 and the PD-1 ligand (PD-L1) exhibits superior anti-tumor responses compared with single-agent therapy. Here, we examined the molecular basis for
Kathleen E Orrell et al.
Nature communications, 11(1), 432-432 (2020-01-25)
Large Clostridial Toxins (LCTs) are a family of six homologous protein toxins that are implicated in severe disease. LCTs infiltrate host cells using a translocation domain (LCT-T) that contains both cell-surface receptor binding sites and a membrane translocation apparatus. Despite
Yasunori Saheki et al.
Nature cell biology, 18(5), 504-515 (2016-04-12)
Acute metabolic changes in plasma membrane (PM) lipids, such as those mediating signalling reactions, are rapidly compensated by homeostatic responses whose molecular basis is poorly understood. Here we show that the extended synaptotagmins (E-Syts), endoplasmic reticulum (ER) proteins that function
Xiaozheng Xu et al.
The Journal of cell biology, 219(6) (2020-05-22)
Blockade antibodies of the immunoinhibitory receptor PD-1 can stimulate the anti-tumor activity of T cells, but clinical benefit is limited to a fraction of patients. Evidence suggests that BTLA, a receptor structurally related to PD-1, may contribute to resistance to
Xuewu Zhang et al.
Cell, 125(6), 1137-1149 (2006-06-17)
The mechanism by which the epidermal growth factor receptor (EGFR) is activated upon dimerization has eluded definition. We find that the EGFR kinase domain can be activated by increasing its local concentration or by mutating a leucine (L834R) in the
Anthony L Shrout et al.
Biochemistry, 42(46), 13379-13385 (2003-11-19)
Transmembrane receptors in the signaling pathways of bacterial chemotaxis systems influence cell motility by forming noncovalent complexes with the cytoplasmic signaling proteins to regulate their activity. The requirements for receptor-mediated activation of CheA, the principal kinase of the Escherichia coli
Hao Zhang et al.
Molecular & cellular proteomics : MCP, 10(6), M110-M110 (2011-03-23)
Her4 is a transmembrane receptor tyrosine kinase belonging to the ErbB-EGFR family. It plays a vital role in the cardiovascular and nervous systems, and mutations in Her4 have been found in melanoma and lung cancer. The kinase domain of Her4
Jee-Yeon Ryu et al.
Biomaterials, 232, 119736-119736 (2020-01-07)
Compared to a plasmid, viral, and other delivery systems, direct Cas9/sgRNA protein delivery has several advantages such as low off-targeting effects and non-integration, but it still has limitations due to low transfer efficiency. As such, the CRISPR/Cas9 system is being
Shodai Togo et al.
APL bioengineering, 4(1), 016103-016103 (2020-02-01)
Programmable cell adhesion with DNA hybridization is a promising approach for fabricating various tissue architectures without sophisticated instrumentation. However, little is known about how this artificial interaction influences the binding of cell adhesion proteins, E-cadherin. In this work, we designed
Thanh X Dang et al.
Journal of structural biology, 152(2), 129-139 (2005-10-26)
To facilitate purification and subsequent structural studies of recombinant proteins the most widely used genetically encoded tag is the histidine tag (His-tag) which specifically binds to N-nitrilotriacetic-acid-chelated nickel ions. Lipids derivatized with a nickel-chelating head group can be mixed with
Ehsan Suleiman et al.
Pharmaceutics, 11(11) (2019-11-23)
Since the first use of liposomes as carriers for antigens, much work has been done to elucidate the mechanisms involved in the encapsulation of vaccine-relevant biomolecules. However, only a few studies have specifically investigated the encapsulation of hydrophilic, non-conformational peptide
M J Rodrigues-Jesus et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 50(1), 67-77 (2019-01-15)
Microlipid vesicles (MLV) have a broad spectrum of applications for the delivery of molecules, ranging from chemical compounds to proteins, in both in vitro and in vivo conditions. In the present study, we developed a new set of nanosize multilayer
Decorating liquid crystal surfaces with proteins for real-time detection of specific protein-protein binding
Hartono D, et al.
Advances in Functional Materials, 19(22), 3574-3579 (2009)
Zhilin Chen et al.
Bioconjugate chemistry, 29(4), 1251-1260 (2018-03-13)
The number and spacing of B-cell epitopes on antigens have a profound impact on the activation of B cells and elicitation of antibody responses, the quantitative aspects of which may be utilized for rational design of vaccines. Ni-chelating liposomes have
Phuong A Nguyen et al.
Methods in cell biology, 128, 223-241 (2015-05-23)
Cell division in prokaryotes and eukaryotes is commonly initiated by the well-controlled binding of proteins to the cytoplasmic side of the cell membrane. However, a precise characterization of the spatiotemporal dynamics of membrane-bound proteins is often difficult to achieve in
C Vénien-Bryan et al.
Biophysical journal, 74(5), 2649-2657 (1998-05-20)
We present here some sensitive optical and mechanical experiments for monitoring the process of formation and growth of two-dimensional (2D) crystals of proteins on a lipid monolayer at an air-water interface. The adsorption of proteins on the lipid monolayer was
Diego De Miguel et al.
Cancers, 11(12) (2019-12-11)
Although TRAIL (TNF-related apoptosis-inducing ligand, also known as Apo2L) was described as capable of inducing apoptosis in transformed cells while sparing normal cells, limited results obtained in clinical trials has limited its use as an anti-tumor agent. Consequently, novel TRAIL
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