; buffer B: 90/10/0.1 MeCN/water/TFA.
Peptides 1-4: Gradient 0% B for 0.2 min, then 0-100% B in 18 min;
flow rate: 3 ml/min. Detection: 214 nm. Peptide 5: Gradient 0% B for
0.2 min, then 0-100% B in 7
activation. Cleavage from the
resin should be carried out using 90% TFA at 0 °C. Using this
approach, desulfation can be reduced to less than 15% [7].
04-12-1251 Fmoc-Tyr(SO3·NnBu4)-OH 1 g
NEW 5 g
NEW Derivative
y
(m
V
)
0.0-6.00 2.550 97.550
6.01-8.00 50 50
-1.00E+04
4.00E+04
9.00E+04
1.40E+05
M
S
In
te
0 1 2 3 4 5
Retention Time (minutes)
Chromatographic Data
No.