commercial reagent contains 93-98% H2SO4, the balance being water. Its effective
concentration is 18 M (36N).
Freezing point: Anhydrous sulfuric acid freezes at 10°C; 98% sulfuric acid freezes at +3°
Applied 500 �l # �90%
Table 2. Effect of sample volume on DNA recovery.
% recovery of
1 �g DNA
% recovery of
10 �g DNA
Sample 100 �l 60 – 70% �99%
Volume 250
reported to
enhance tyrosine protein phosphorylation and activation
of ERK1/2 and MEK1/2. Purity: >90% by HPLC.
Cat. No. 506350 2 mg
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lysate or purified
enzyme solution in a well.
2. Add the 90 µl of Buffer to the well.
3. Start the reaction with the addition of 10 µl of the
2 mM substrate solution using
apoptosis
trifluoroacetate salt induction in intact hematopoietic cell lines.
((L-Asp)2 Rhodamine 110-TFA)
Minimum 98%
Bisbenzimide H 33258 Membrane-permeable, fluorescent DNA stain with low cytotoxicity
Impaired in
Mice Bearing a Targeted Mutation in the Protein
Phosphatase 1cγ. Gene. Dev. Biol., 205, 98-110
(1999).
3. Dawson, J.F., et al., Mutation of the toxin binding
site of PP-1c: comparison with
References
1. ISCO Staff, ISCO Tables, 10th edition, p. 35 (1989)
2. Dahlberg, A.E., et al., J. Mol. Biol. 41, 139 (1969)
1/98
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
ionic strength, with pH 7-9.1
A4046 Page 2 of 3
04/07/98 - CKV
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM
ionic strength, with pH 7-9.1
A4046 Page 2 of 3
04/07/98 - CKV
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM
ionic strength, with pH 7-9.1
A4046 Page 2 of 3
04/07/98 - CKV
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM
shaking speed of 90 - 100 rpm.
Cultures were passaged twice per week (5 x 106 cells/mL) and
were considered fully adapted after 6 passages, as indicated
by sustained viabilities above 90%, and at least
156-159 (1987).
2. Puissant, C. and Houdebine, L.M., BioTechniques,
8, p. 148-149 (1990).
3. Kedzierski, W. and Porter, J.C., BioTechniques, 10,
p. 210-214 (1991).
7/98
acid from UDP-glucose per min in the presence of NAD+ at 25°C, pH 8.7. Purity: >90% by SDS-PAGE.
Cat. No. 670121 2 units €98
10 units €358
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