90220-001

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Showing 1-8 of 8 results for "90220-001" within Papers

A microfluidic device for dry sample preservation in remote settings.

Stefano Begolo et al.

Lab on a chip, 13(22), 4331-4342 (2013-09-24)

This paper describes a microfluidic device for dry preservation of biological specimens at room temperature that incorporates chemical stabilization matrices. Long-term stabilization of samples is crucial for remote medical analysis, biosurveillance, and archiving, but the current paradigm for transporting remotely

Adjunctive dexamethasone affects the expression of genes related to inflammation, neurogenesis and apoptosis in infant rat pneumococcal meningitis.

Cornelia Blaser et al.

PloS one, 6(3), e17840-e17840 (2011-03-18)

Streptococcus pneumoniae is the most common pathogen causing non-epidemic bacterial meningitis worldwide. The immune response and inflammatory processes contribute to the pathophysiology. Hence, the anti-inflammatory dexamethasone is advocated as adjuvant treatment although its clinical efficacy remains a question at issue.

Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak.

Stephen K Gire et al.

Science (New York, N.Y.), 345(6202), 1369-1372 (2014-09-13)

In its largest outbreak, Ebola virus disease is spreading through Guinea, Liberia, Sierra Leone, and Nigeria. We sequenced 99 Ebola virus genomes from 78 patients in Sierra Leone to ~2000× coverage. We observed a rapid accumulation of interhost and intrahost

Stabilization of RNA through absorption by functionalized mesoporous silicate nanospheres.

Brandy J Johnson et al.

PloS one, 7(11), e50356-e50356 (2012-12-12)

The potential for encapsulating RNA within tunable, semi-permeable structures for storage and transportation purposes offers an interesting approach to the reduction of stringent storage requirements that often hamper the field application of genetic analysis methods. In this study, we assessed

Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis.

Gilberto E Hernandez et al.

BioTechniques, 47(2), 667-668 (2009-09-10)

RNA integrity is a critical factor in obtaining meaningful gene expression data. Current methodologies rely on maintaining samples in cold environments during collection, transport, processing, and storage procedures, which are also extremely time-sensitive. Several RNA storage products are commercially available

Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.

Wen Huang et al.

PloS one, 7(1), e30244-e30244 (2012-02-01)

In this study, mRNA-Seq was used to characterize and compare the leukocyte transcriptomes from two taurine breeds (Holstein and Jersey), and one indicine breed (Cholistani). At the genomic level, we identified breed-specific base changes in protein coding regions. Among 7,793,425

Laboratory operations, specimen processing, and handling for viral load testing and surveillance.

Adrian Puren et al.

The Journal of infectious diseases, 201 Suppl 1, S27-S36 (2010-03-17)

RNA remains the most informative and accurate biomarker for human immunodeficiency virus type 1 load diagnostics and for surveillance of drug resistance markers. Viral load testing by nucleic acid amplification currently is a complex and expensive test that is restricted

Post-extraction stabilization of HIV viral RNA for quantitative molecular tests.

Daniel S Stevens et al.

Journal of virological methods, 182(1-2), 104-110 (2012-03-22)

Two approaches to stabilize viral nucleic acid in processed clinical specimens were evaluated. HIV-1 RNA extracted from clinical specimens was stabilized in a dry matrix in a commercial product (RNAstable, Biomatrica, San Diego, CA, USA) and in a reverse-transcription reaction

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