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Product Information Sheet-11888412001
volume of 50 μl 11 647 679 001
1,000 U, 4 x 250 U, 800 reactions in a final volume of 50 μl 11 647 687 001
Uracil-DNA Glycosylase,
heat-labile
100 U, 1 U/μl 11 775 367 001
500
Enzymatic Assay of ACYLASE I
570 nm for each of the
vials using a suitable spectrophotometer.
SSACET01 Page 4 of 5
Revised: 11/11/95
Enzymatic Assay of ACYLASE I
(EC 3.5.1.14)
Sigma Prod. No. A-2156
CALCULATIONS:
Product Information Sheet-dntpm-ro
001
100 ml, 4 x 25 ml 03 315 843 001
Taq DNA Polymerase, 5 U/µl 100 U, 5 U/μl, 80 reactions 11 146 165 001
500 U, 5 U/μl, 400 reactions 11 146 173 001
4 x 250 U, 5 U/μl
DECLARATION OF CONFORMITY BenchMixer Vortex mixer and Mortexer Vortex mixer
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
EN 61000-4-3:02 +A1:02
EN 61000-4-4:04
EN 61000-4-5:95 +A1:01
EN 61000-4-6:96 +A1:01
DECLARATION OF CONFORMITY Mini BlotBoy and BlotBoy 3D mixers
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
EN 61000-4-3:02 +A1:02
EN 61000-4-4:04
EN 61000-4-5:95 +A1:01
EN 61000-4-6:96 +A1:01
DECLARATION OF CONFORMITY Benchmark MyBlock Digital Drybath
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
EN 61000-4-3:02 +A1:02
EN 61000-4-4:04
EN 61000-4-5:95 +A1:01
EN 61000-4-6:96 +A1:01
Product Information Sheet-03734927001
Pre-Incubation 92 – 95(1) 2 min 1
Denaturation
Annealing
Elongation
92 – 95(1)
55 – 65(2)
72
15 – 30 sec
30 – 60 sec
45 sec – 3 min
25 – 30
Final Elongation
DECLARATION OF CONFORMITY -Benchmark MultiTherm Shaker
EN 61000-3-3:95+A1:01+A2:05
EN 61000-4-2:95 +A1:98 +A2:01
EN 61000-4-3:02 +A1:02
EN 61000-4-4:04
EN 61000-4-5:95 +A1:01
EN 61000-4-6:96 +A1:01
Automation Guide WGA
Initial Denaturation 95 °C 3 minutes 1
Denaturation 94 °C 15 seconds
Annealing/Extension 65 °C 5 minutes
14
Hold 4 °C Indefinitely
Product Information Sheet-ftaq-ro
Pre-Incubation/activation 95 4 min(1) 1
Denaturation
Annealing
Elongation
95
45 – 65(3)
72
30 sec
30 sec
45 sec – 3 min(4)
30 – 40(2)
Final Elongation 72 7 min 1
Cooling 4 indefinitely
Verfication summary-03-0202-00
3 MRD (1:4) 17.73 0.7 4% 70.91 2.7 4% -
3 1:8 8.46 1.2 14% 67.70 9.5 14% 95%
3 1:16 4.26 0.7 16% 68.22 11.0 16% 96%
2 1:32 2.10 0.4 19% 67.04 12.8 19% 95%
HMN686703
Verfication summary-03-0202-00
3 MRD (1:4) 17.73 0.7 4% 70.91 2.7 4% -
3 1:8 8.46 1.2 14% 67.70 9.5 14% 95%
3 1:16 4.26 0.7 16% 68.22 11.0 16% 96%
2 1:32 2.10 0.4 19% 67.04 12.8 19% 95%
HMN686703
(I5272) - Enzyme Assay
12-Crown-4 (I5272) - Enzyme Assay
L5272 Page 1 of 4
SSLAMI01
Revised: 05/11/95
SIGMA QUALITY CONTROL TEST
PROCEDURE
Enzymatic Assay of LAMINARINASE1
(EC 3.2.1.6)
PRINCIPLE:
Laminarin
TOTAL DIETARY FIBER ASSAY KIT / GESAMT-BALLASTSTOFFBESTIMMUNGS-KIT
mischen.
4. Die Bechergläser mit Alufolie zudecken und in ein kochendes Wasserbad stellen. Bechergläser alle 5 Minuten
leicht schwenken. Nach dem die Innentemperatur der Bechergläser 95°C erreicht
Product Information Sheet - TDFC10
mischen.
4. Die Bechergläser mit Alufolie zudecken und in ein kochendes Wasserbad stellen. Bechergläser alle 5 Minuten
leicht schwenken. Nach dem die Innentemperatur der Bechergläser 95°C erreicht
Product Information Sheet-11004760001
Reagents, kits
Digoxigenin-11-dUTP, alkali-stable 25 nmol, 25 µl, 1 mM 11 093 088 910
125 nmol, 125 µl, 1 mM 11 558 706 910
5 x 125 nmol, 5x 125 µl, 1 mM 11 570 013 910
Digoxigenin-11-dUTP, alkali-labile
(L5272) - Enzyme Assay
acetate, 0.50% (w/v) laminarin
and 0.075 - 0.15 unit laminarinase.
L5272 Page 4 of 4
SSLAMI01
Revised: 05/11/95
Enzymatic Assay of LAMINARINASE1
(EC 3.2.1.6)
REFERENCES:
Somogyi, M. (
(L9259) - Enzyme Assay
acetate, 0.50% (w/v) laminarin
and 0.075 - 0.15 unit laminarinase.
L9259 Page 4 of 4
SSLAMI01
Revised: 05/11/95
Enzymatic Assay of LAMINARINASE1
(EC 3.2.1.6)
REFERENCES:
Somogyi, M. (
Product Information Sheet-dutp-ro
U, 1 U/μl 11 775 367 001
500 U, 1 U/μl 11 775 375 001
Taq DNA Polymerase,
5 U/µl
100 U, 5 U/μl
80 reactions
11 146 165 001
500 U, 5 U/μl
400 reactions
TOTAL DIETARY FIBER ASSAY KIT / GESAMT-BALLASTSTOFFBESTIMMUNGS-KIT
mischen.
4. Die Bechergläser mit Alufolie zudecken und in ein kochendes Wasserbad stellen. Bechergläser alle 5 Minuten
leicht schwenken. Nach dem die Innentemperatur der Bechergläser 95°C erreicht
Data Sheet - SRP5204
Biol. Chem., 258, 930, (1983).
4. Chevalier, D. et al., Purification of myelin basic
protein from bovine brain. Protein Expr. Purif., 18,
229-234 (2000).
FF,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC.
Product Information Sheet - P5380
minutes (activity
remaining):19
• pH 6 ≈ 70%
• pH 7 ≈ 80%
• pH 7.5-10 ≈ 95%
• pH 10.5 ≈ 90%
• pH 11 ≈ 70%
Product Information Sheet-taq-ro
glycerol
(v/v), pH 8.0 (+4°C).
11 146 165 001 1 vial,
20 μl
11 146 173 001 2 vials,
50 μl each
11 148 432 001 4 vials,
50 μl each
11 596 594 001 10
Product Information Sheet-fpcr-ro
Time Number of Cycles
Pre-Incubation/activation 95(1) 4 min 1
Denaturation
Annealing
Elongation
95
45 – 65(3)
72
30 sec
30 sec
45 sec – 3 min(4)
30 – 40(2)
Final Elongation 72 7 min
TOTAL DIETARY FIBER ASSAY KIT / GESAMT-BALLASTSTOFFBESTIMMUNGS-KIT
volumen con etanol al 95%. Mezclar y llevar a volumen
de nuevo con etanol al 95% si es necesario. Mix.
2. Buffer fosfato, 0,08 M, pH 6,0,
disolver 1,4 g de Na 2HPO
Product Information Sheet-fpcrn-ro
Pre-Incubation/activation 95 4 min(1) 1
Denaturation
Annealing
Elongation
95
45 – 65(3)
72
30 sec
30 sec
45 sec – 3 min(4)
30 – 40(2)
Final Elongation 72 7 min 1
Cooling 4 indefinitely
Data Sheet - SRP5106
multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
p 18INK4C43
72
95
130
170
56
34
26
References
1. Ravichandran, N. et al., Structure-based
Product Information Sheet-udghl-ro
Storage Buffer: 20 mM Tris-HCl, pH 8.0 (+4°C),
0.1 mM EDTA, 100 mM KCl, 1 mM DTT,
50% glycerol (v/v), 0.05% Tween 20 (v/v).
11 775 367 001 1 vial,
100 µl
11 775 375 001 1 vial
Data Sheet - SRP5073
multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
PTPN12
34
43
72
95
130
170
56
Figure 2.
Specific Activity of Typical Lot
2,520–3,410
Data Sheet - SRP5080
handling
and multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
43
72
95
130
170
56
PTPRF
34
26
17
Figure 2.
Specific Activity of Typical
Page 1 of 77