95-50-1

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Showing 1-30 of 3267 results for "95-50-1" within Technical Documents

Cycles Initial-denaturation 95°C 3 min 1 Denaturation 95°C 15 sec 5 Extension 74°C 60 sec/kb Denaturation 95°C 15 sec 5 Extension 72°C 60 sec/kb Denaturation 95°C 15 sec 5 Extension 70°

The Effective Use of Protein Kinase Inhibitors

60 71 50 30 22 1 95 59 6 72 36 93 84 94 14 26 90 101 82 61 48 75 38 99 86 8 6 54 66 10 83 105 106 103 99 98 103 104

Data Sheet - p5221

Cycles Initial-denaturation 95°C 3 min 1 Denaturation 95°C 15 sec 5 Extension 74°C 60 sec/kb Denaturation 95°C 15 sec 5 Extension 72°C 60 sec/kb Denaturation 95°C 15 sec 5 Extension 70°

The Effective Use of Protein Kinase Inhibitors

60 71 50 30 22 1 95 59 6 72 36 93 84 94 14 26 90 101 82 61 48 75 38 99 86 8 6 54 66 10 83 105 106 103 99 98 103 104

Amyloglucosidase from Aspergillus niger (A3042)

Assay SSSTAR04 Page 1 of 4 Revised: 09/07/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of AMYLOGLUCOSIDASE1 (EC 3.2.1.3) PRINCIPLE: Step 1: Starch + H2O Amyloglucosidase

Amyloglucosidase from Aspergillus niger (A3514)

Assay SSSTAR04 Page 1 of 4 Revised: 09/07/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of AMYLOGLUCOSIDASE1 (EC 3.2.1.3) PRINCIPLE: Step 1: Starch + H2O Amyloglucosidase

Amyloglucosidase from Rhizopus sp (A7255)

Assay SSSTAR04 Page 1 of 4 Revised: 09/07/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of AMYLOGLUCOSIDASE1 (EC 3.2.1.3) PRINCIPLE: Step 1: Starch + H2O Amyloglucosidase

Data Sheet - P6361

.4 M urea and 50% acetonitrile for 1 hour at 95 °C and pH 7.0, the enzyme retains 70% and 90%, respectively, of its activity. More than 50% of its activity is observed when incubated at 95 °C and pH 7.0

Product Information Sheet-11888412001

of Cycles UNG digestion 20 2 min 1 UNG inactivation and denaturation of the template 95 2 min 1 Denaturation Annealing Elongation 95 50 – 70 72 45 sec 1 min 2 min 30 Elongation

Suitability Assay for COLLAGEN

COLLAGEN REAGENTS: (continued) G. 50% (v/v) 1-Propanol Solution (Prepare 100 ml in deionized water using 1-Propanol, Sigma Stock No. 29,328-8.) H. Ninhydrin Color Reagent (NCR) (Immediately

Data Sheet - SCT129

determined by the user. 1. Dilute BioTracker™ synaptic dye to a final concentration of 4 uM in 50 mM Tyrode solution. Place the coverslip with your cells in this solution for 1 minute at room temperature

Product Information Sheet - 11585541001

incubation +20°C 1 - 10 min 1 × UNG inactivation +95°C 2 min 1 × denaturation +95°C 10 s annealing +55°C to + 65°C 30 s 10 × elongation +72°C 1 min 30 s a)

BioTracker¿ 640 Red C2M(FM5-95) Synaptic Dye

Product Information Sheet-03734927001

Pre-Incubation 92 – 95(1) 2 min 1 Denaturation Annealing Elongation 92 – 95(1) 55 – 65(2) 72 15 – 30 sec 30 – 60 sec 45 sec – 3 min 10 Denaturation Annealing Elongation

(L0763) - Enzyme Assay

to pH 7.7 at 37ΕC with 1 M HCl.) B. Olive Oil Substrate Solution (Olive Oil) (Use Sigma Lipase Substrate, Sigma Stock No. 800-1.) C. 95% Ethanol (Nondenatured) (Prepare 50 ml in deionized water using

(I0763) - Enzyme Assay

to pH 7.7 at 37EC with 1 M HCl.) B. Olive Oil Substrate Solution (Olive Oil) (Use Sigma Lipase Substrate, Sigma Stock No. 800-1.) C. 95% Ethanol (Nondenatured) (Prepare 50 ml in deionized water using

Data Sheet - R4506

Activity: 10,000 units/ml Cutting: 100% Ligation: >95% Recutting: >95% No degradation detected with >50 units for 16 hrs. Fold over digestion: 800 (50 units x 16 hrs.) Package Size: 2,000 units Unit

Data Sheet

1000009 25 34 x 50 60 1000D12 50 42 x 60 120 1000D16 100 50 x 70 60 1000D18 150 60 x 80 90 1000D21 250 70 x 95 60 1000D23 400 80 x

Product Information Sheet - I4256

of precipitation from 25 to 325 g of immuno- globulin versus 50 l of anti-cat whole serum. Purity (SDS-PAGE): Not less than 95%. Sigma warrants that its products conform to the information contained

Poster: Purification with Millipore MultiScreen 384SEQ Plates on Beckman-Coulter’s Biomek FX for High Quality Direct Sequencing in High-Throughput Genomics

interference required. •Data confidence is higher: Phred 30 = 1 mistake in 1,000 bases Phred 40 = 1 mistake in 10,000 bases Phred 50 = 1 mistake in 100,000 bases Significant cost savings - fewer data

Data Sheet - SRP5145

stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol. Molecular mass: ∼65 kDa Purity: 70–95% (SDS-PAGE, see Figure 1) Precautions

(G5507) - Enzyme Assay

Prepare 50 ml in deionized water using Gly-Gly, Hydrochloride, Sigma Prod. No. G-1127. Adjust to pH 8.6 at 30°C with 1 M NaOH.) B. 0.70 P0� �Nicotinamide Adenine Dinucleotide, Reduced )RUP�� 1$'+� �'

Application Note - qPCR Assays Using the LightCycler 2.0 Instrument

0.337 0.237 0.137 0.037 40 45 50 55 60 65 70 75 80 85 90 95 Temperature (ºC) Fl uo re sc en ce ( 53 0 nm ) 7.3 6.6 5.9 5.2 4.5 3.8 3.1 2.4 1.7 1 0.3 2 6 10 14 18 22

Amicon® Ultra-4 Centrifugal Filter Devices User Guide

α-Chymotrypsinogen (1 mg/mL) 25,000 3K > 95 > 95 40 Cytochrome c (0.25 mg/mL) 12,400 > 95 > 95 40 Vitamin B-12 (0.2 mg/mL) 1,350 1 mg/mL) 25,000 10K > 95 > 95 15 Cytochrome

Product Information Sheet-ftaq-ro

-Incubation/activation 95 4 min(1) 1 Denaturation Annealing Elongation 95 45 – 65(3) 72 30 sec 30 sec 45 sec – 3 min(4) 30 – 40(2) Final Elongation 72 7 min 1 Cooling 4 indefinitely

User Guide (IFU) - Papanicolaou Staining System (Procedure No. HT40)

EA 50, OU solution de coloration de Papanicolaou EA 65 pendant 2,5 minutes. 13. Plonger 10 fois dans deux bains d’alcool de qualité réactif à 95 %. 14. Alcool de qualité réactif à 100 % pendant 1 minute

Product Information Sheet - A7718

. 1. Begin heating distilled deionized water to 95-100 ºC in a clean container filled with sufficient water to cover slides in a slide staining rack. 2. Fill a humidity chamber with ~50 ml water

Data Sheet - I4256

continuous arc of precipitation from 25 to 325 µg of immunoglobulin versus 50 µl of anti-cat whole serum. Purity (SDS-PAGE): Not less than 95%. Sigma warrants that its products conform to the information

Data Sheet - SRP5208

stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol. Molecular mass: ∼50 kDa Purity: 70–95% (SDS-PAGE, see Figure 1) Precautions

Data Sheet - R2630

Unit Definition One unit is the enzyme activity that completely cleaves 1 µg λ DNA in 1 hr. at 37EC in a total volume of 50 µl of Buffer SB for restriction enzymes. Specificity Ban II recognizes the

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