95-50-1

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Showing 31-60 of 3273 results for "95-50-1" within Technical Documents

repeated handling and multiple freeze/thaw cycles. Figure 1. SDS-PAGE Gel of Typical Lot 70–95% (densitometry) PTPN12 34 43 72 95 130 170 56 Figure 2. Specific Activity of Typical

(L7386) - Enzyme Assay

pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 50 mM Tris HCl Buffer with 145 mM Sodium Chloride, pH 7.5 at 37�C (Prepare 1 liter in deionized water using

(L4402) - Enzyme Assay

pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 50 mM Tris HCl Buffer with 145 mM Sodium Chloride, pH 7.5 at 37�C (Prepare 1 liter in deionized water using

Product Information Sheet-dntpm-ro

Pre-Incubation 92 – 95(1) 2 min 1 Denaturation Annealing Elongation 92 – 95(1) 55 – 65(2) 72(3) 15 – 30 sec 30 – 60 sec 45 sec – 3 min 10 Denaturation Annealing Elongation

Data Sheet - SRP5078

stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol. Molecular mass: ∼96 kDa Purity: 70–95% (SDS-PAGE, see Figure 1) Specific

Data Sheet - R8257

at 37 °°°°C. 50 mM Tris-HCl 100 mM NaCl 10 mM MgCl2 1 mM dithioerythritol (DTE) pH 7.5 Absence of non-specific endonuclease activities 1 µg λ DNA is incubated for 16 hrs. in 50 µl of

Product Information Sheet - M2185

9802 Ethanol, 95+% E 7148 20X Saline-sodium citrate (SSC) buffer S 6639 Slide staining chambers/racks S 6141 Orbital shaker (The Belly Dancer) Z36,760-5 Preparation Instructions 1. Printing of

Data Sheet - R5757

Nde II Storage and Dilution Buffer Composition 20 mM Tris-HCl 50 mM NaCl 0.1 mM EDTA 1 mM dithioerythritol 0.02% Thesit (v/v) 50% (v/v) glycerol pH 7.5 Quality Control Testing 2x Digestion

User Guide (IFU) - Scott's Tap Water (Procedure No. S5134)

water. 7. Reagent Alcohol, 95% for 10 dips. 8. Counterstain in Papanicolaou Stain OG-6 for 1.5 minutes. 9. Reagent Alcohol, 95% for 10 dips. 10. Papanicolaou Stain EA 50, or Papanicolaou Stain EA 65

Data Sheet - R3379

Unit Definition One unit is the enzyme activity that completely cleaves 1 µg λDNA in 1 hr. at 37 °C in a total volume of 50 µl of Buffer SB for restriction endonucleases. Specificity Ava I recognizes

(C4129) - Enzyme Assay

a-Chymotrypsin from bovine pancreas (C4129) - Enzyme Assay Revised: 08/16/95 Page 1 of 3 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of CHYMOTRYPSIN1 (EC 3.4.21.1) PRINCIPLE

Bulletin - S7934

°C. 1. Begin heating distilled deionized water to 95-100 °C in a clean container filled with sufficient water to cover slides in a slide staining rack. 2. Fill a humidity chamber with ~50 ml water

Data Sheet - R6634

generates fragments with 5'-cohesive termini.1 Alw 44 I is an isoschizomer to Sno I. Comments Digestion Buffer SA is supplied as a 10x concentrate. 1-50 units of Alw 44 I can be heat inactivated

Data Sheet - I4385 - Lot 095H8885

of precipitation in the gamma region at 50 and 250 J�RI immunoglobulin versus anti-monkey whole serum and anti-monkey IgG. Purity (SDS-PAGE): Not less than 95%. Sigma warrants that its products conform

(I4402) - Enzyme Assay

pH 7.5, A620nm, Light path = 1 cm METHOD: Turbidimetric Rate Determination REAGENTS: A. 50 mM Tris HCl Buffer with 145 mM Sodium Chloride, pH 7.5 at 37EC (Prepare 1 liter in deionized water using

Data Sheet - R1885

Activity: 10,000 units/ml Cutting: 100% Ligation: >95% Recutting: >95% No degradation detected with >50 units for 16 hrs. Fold over digestion: 800 (50 units x 16 hrs.) Package Size: 250 units Unit

Product Information - T6640

with 1 M NaOH.) B. 0.25 mM Nα-Benzoyl-L-Arginine Ethyl Ester Solution (BAEE) (Prepare 50 ml in Reagent A using Nα-Benzoyl-L-Arginine Ethyl Ester, Hydrochloride, Sigma Prod. No. B-4500.) C. 1 mM Hydrochloric

Product Information - T4665

Product Information -T8658

Determination of sugars in beverages

g/l for the first calibration solution. Select the “Absorbance” field in the “1“ line. Fill calibration solution 1 into a 50-mm rectangular cell and insert cell into the cell compartment. The measurement

Data Sheet - SRP5080

stored in 20 mM MOPS, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, and 30% glycerol. Molecular mass: ∼93 kDa Purity: 70–95% (SDS-PAGE, see Figure 1) Specific Activity: 386–522

pDADMAC flocculant reagent for use with Clarisolve® depth filters depth filter system

Step According to pDADMAC Dosage Time (min) %A %B Valve 0 95 5 waste 1 95 5 detector 4 50 50 detector 5 95 5 detector 10 95 5 detector

Amicon® Ultra-0.5 Centrifugal Filter Devices for volumes up to 500 μL

mg/mL) 1,350 1 mg/mL) 67,000 30K > 95 10 Ovalbumin (1 mg/mL) 45,000 > 95 10 Cytochrome c (0.25 mg/mL) 12,400 1 mg/mL) 67,000 50K > 95 10 Ovalbumin (1 mg/mL) 45,000 ~ 40 10 Cytochrome

Data Sheet - R4640

One unit is the enzyme activity that completely cleaves 1 µg λDNA in 1 hr. at 37 °C in a total volume of 50 µl of Buffer SH for restriction enzymes.1 µg pBR322 DNA is digested completely by 2 units of Eco

Data Sheet - SRP5176

Avoid repeated handling and multiple freeze/thaw cycles. Figure 1. SDS-PAGE Gel of Typical Lot 50–95% (densitometry) References 1. Halazonetis, T.D. et al., c-Jun dimerizes with itself and with

(G8887) - Enzyme Assay

deionized water.) G. 50 mM Tris HCl Buffer, pH 8.5 at 30°C (Enzyme Diluent) (Prepare 50 ml in deionized water using Trizma Base, Sigma Prod. No. T-1503. Adjust to pH 8.5 at 30°C with 1 M HCl.) H. Glucokinase

Data Sheet - R0629

mM Tris-HCl 10 mM MgCl2 1 mM dithioerythritol (DTE) pH 7.5 Quality Control Testing Absence of unspecific endonuclease activities: 1 µg λDNA is incubated for 16 hrs. in 50 µl buffer SL with excess

Data Sheet - R4257

units/ml Cutting: 100% Ligation: >95% Recutting: >95% No degradation detected with >10 units for 16 hrs. Fold over digestion: 160 (10 units x 16 hrs.) Package Size: 50 units Unit Definition One unit

Amicon® Ultra-15 Centrifugal Filter Devices User Guide

α-Chymotrypsinogen (1 mg/mL) 25,000 3K > 95 > 95 60 Cytochrome c (0.25 mg/mL) 12,400 > 95 > 95 60 Vitamin B-12 (0.2 mg/mL) 1,350 1 mg/mL) 25,000 10K > 95 > 95 30 Cytochrome c

MOUSE ANTI-PROSTATE SPECIFIC ANTIGEN MONOCLONAL ANTIBODY

ethyl alcohol, 80% ethyl alcohol, 90% ethyl alcohol, 95% ethyl alcohol, 95% ethyl alcohol, 100% ethyl alcohol, 100% ethyl alcohol, 50% Xylene/50% ethyl alcohol, Xylene, Xylene. 13. Mount slides mounted

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