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Showing 1-30 of 320 results for "A4418" within Papers
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 74(6), 16-23 (2013-01-09)
A scheme of isolation and purification of the enzyme with alpha-L-rhamnosidase activity from culture liquid Cryptococcus albidus 1001 has been developed. It included fractionation by ammonium sulfate and chromatography on TSK-gels Toyopearl HW-60, Fractogel TSK DEAE-650-s and Sepharose 6B. The
Qiao-Yi Shen et al.
Journal of chromatography. A, 1397, 73-80 (2015-04-22)
Herein, a novel strategy was developed to separate and prepare target protein from complex sample by free-flow electrophoresis (FFE), which mainly based on the charge-to-mass ratio (C/M) analysis of proteins. The C/M values of three model proteins, namely Cytochrome C
Roni Yosipovich et al.
Vaccine, 33(3), 472-478 (2014-12-03)
In the first 10-14 days of a chick's life, protection is conferred by maternal antibodies. Further broiler protection is achieved by active vaccination. However, the high level of maternal antibodies interferes with the induction of an effective immune response by
Yan He et al.
Journal of chromatography. A, 1408, 261-266 (2015-07-22)
This paper describes the use of an anionic ion-pair reagent (IPR) to impove the ultraviolet (UV) detection and hydrophobic retention of polar and UV transparent cations. Anionic IPR added to the mobile phase forms an ion-pair with cations. Formation of
Simon Gareth Broadley et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 11), 1450-1459 (2012-10-24)
MshB, a zinc-based deacetylase, catalyses a step in the mycothiol biosynthetic pathway that involves the deacetylation of 1-O-(2-acetamido-2-deoxy-α-D-glucopyranosyl)-D-myo-inositol (GlcNAc-Ins), via cleavage of an amide bond, to 1-O-(2-amino-2-deoxy-α-D-glucopyranosyl)-D-myo-inositol (GlcN-Ins) and acetate. In this study, MshB was expressed, purified and crystallized. A
Gilbert Eriani et al.
PloS one, 10(6), e0130042-e0130042 (2015-06-13)
Aminoacyl-tRNA synthetases (AARSs) constitute a family of RNA-binding proteins, that participate in the translation of the genetic code, by covalently linking amino acids to appropriate tRNAs. Due to their fundamental importance for cell life, AARSs are likely to be one
Anne Berit Dyve Lingelem et al.
PloS one, 10(5), e0129214-e0129214 (2015-05-29)
The heat shock protein 90 (Hsp90) inhibitor geldanamycin (GA) has been shown to alter endosomal sorting, diverting cargo destined for the recycling pathway into the lysosomal pathway. Here we investigated whether GA also affects the sorting of cargo into the
Xin Wang et al.
ACS nano, 8(12), 12151-12166 (2014-12-02)
Chemoresistance is a primary cause of treatment failure in cancer and a common property of tumor-initiating cancer stem cells. Overcoming mechanisms of chemoresistance, particularly in cancer stem cells, can markedly enhance cancer therapy and prevent recurrence and metastasis. This study
Silvia Llopis et al.
PloS one, 9(5), e98094-e98094 (2014-06-01)
Saccharomyces cerevisiae plays a beneficial role in health because of its intrinsic nutritional value and bio-functional properties, which is why it is also used as a dietary supplement. However, the perception that S. cerevisiae is harmless has changed due to
Ângela Sousa et al.
Journal of chromatography. A, 1366, 110-119 (2014-09-30)
Histamine and agmatine amino acid derivatives were immobilized into monolithic disks, in order to combine the specificity and selectivity of the ligand with the high mass transfer and binding capacity offered by monolithic supports, to purify potential plasmid DNA biopharmaceuticals.
Taro Masuda et al.
Bioscience, biotechnology, and biochemistry, 79(1), 74-81 (2014-10-16)
Iron is one of the essential trace elements for humans. In this study, the iron contents in fresh, dried, and toasted nori (Pyropia yezoensis) were analyzed. The mean iron content of fresh, dried, and toasted nori were 19.0, 22.6, and
Adrian Weisz et al.
Journal of chromatography. A, 1380, 120-129 (2015-01-17)
Three low-level subsidiary color impurities (A, B, and C) often present in batches of the color additive D&C Red No. 33 (R33, Acid Red 33, Colour Index No. 17200) were separated from a portion of R33 by spiral high-speed counter-current
Ryo Nasuno et al.
PloS one, 9(11), e113788-e113788 (2014-11-26)
The budding yeast Saccharomyces cerevisiae possesses various defense mechanisms against environmental stresses that generate reactive oxygen species, leading to growth inhibition or cell death. Our recent study showed a novel antioxidative mechanism mediated by nitric oxide (NO) in yeast cells
Alexander N Wein et al.
Scientific reports, 5, 16267-16267 (2015-11-21)
Anthrax lethal toxin (LT) is an A-B type toxin secreted by Bacillus anthracis, consisting of the cellular binding moiety, protective antigen (PA), and the catalytic moiety, lethal factor (LF). To target cells, PA binds to cell-surface receptors and is then
Rémi Peyraud et al.
Bio-protocol, 7(10), e2289-e2289 (2017-05-20)
Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this
Ankita Khanna et al.
Environmental science and pollution research international, 20(8), 5692-5707 (2013-03-07)
Wastewater released from textile industries causes water pollution, and it needs to be treated before discharge to the environment by cost effective technologies. Solar photocatalysis is a promising technology for the treatment of dye wastewater. The Ag@TiO2 nanoparticles comprising of
Marc A DePaul et al.
Scientific reports, 5, 16795-16795 (2015-11-20)
Following spinal cord injury (SCI), immune-mediated secondary processes exacerbate the extent of permanent neurological deficits. We investigated the capacity of adult bone marrow-derived stem cells, which exhibit immunomodulatory properties, to alter inflammation and promote recovery following SCI. In vitro, we
Sruthi Purushothaman et al.
Journal of proteomics, 112, 113-124 (2014-09-03)
The extensive arm regeneration of brittle stars following amputation is becoming increasingly recognized as a model system for understanding cellular differentiation and regeneration in a whole animal context. In this study we have used the emerging brittle star model Amphiura
Mei Liu et al.
Biotechnology for biofuels, 8, 132-132 (2015-09-05)
Contamination of corn mash by lactic acid bacteria (LAB) reduces the efficiency of the ethanol fermentation process. The industry relies heavily on antibiotics for contamination control and there is a need to develop alternative methods. The goals of this study
Kazunori Sugiura et al.
Biochemical and biophysical research communications, 457(3), 242-248 (2015-01-17)
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004))
W Michael Dunne et al.
Journal of clinical microbiology, 52(10), 3654-3659 (2014-08-01)
The identification of mycobacteria outside biocontainment facilities requires that the organisms first be rendered inactive. Exposure to 70% ethanol (EtOH) either before or after mechanical disruption was evaluated in order to establish a safe, effective, and rapid inactivation protocol that
O A Adaramoye et al.
The West Indian medical journal, 63(5), 408-415 (2015-03-18)
To evaluate and compare the antioxidant and acetylcholinestrase (AChE)-inhibitory properties of aerial parts of Strophanthus preussii (leaves, stem and root named as SPL, SPS and SPR, respectively) while catechin served as standard. The antioxidant and AchE-inhibitory properties of the methanol
Vaishnavi Babu et al.
Journal of thrombosis and thrombolysis, 39(1), 71-78 (2014-06-06)
Streptokinase (SK) is an extracellular enzyme secreted by various strains of β-hemolytic Streptococci. The main focus of the current study is to evaluate the in vitro thrombolytic activity of purified SK extracted from Streptococcus equinus VIT_VB2 (Accession no. JX406835) isolated
Cheng Jiang et al.
Analytica chimica acta, 766, 88-93 (2013-02-23)
T4 polynucleotide kinase (PNK) plays a critical role in various cellular events. Here, we describe a novel colorimetric strategy for estimating the activity of PNK and screening its inhibitors taking advantage of the efficient cleavage of λ exonuclease and the
Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization
David S Powlson, Chris J Dawson
Soil use and management, 38(1), 622-634 (2022)
Okhil K Nag et al.
Journal of pharmaceutical sciences, 104(1), 114-123 (2014-11-14)
We report the synthesis of an acyl-anchored superhydrophilic polymer (SHP) for external surface modification of liposome surface. N¹-(2-aminoethyl)-N⁴-hexadecyl-2-tetradecylsuccinamide conjugated with SHP (HDAS-SHP) was synthesized and used for modifying the liposome surface. Unlike polyethylene glycol (PEG)-phospholipids, which are commonly used for
J F A Valente et al.
Analytical biochemistry, 459, 61-69 (2014-05-28)
The p53 tumor suppressor gene has been widely explored for gene therapy as an alternative to the common treatments. Recently, the supercoiled conformation of a p53-encoding plasmid proved to be more efficient in cell transfection and protein expression than the
Francisco Gasteazoro et al.
Analytical biochemistry, 471, 44-50 (2014-12-03)
Ethylene is a key molecule in organic synthesis currently produced by steam cracking of fossil hydrocarbons. In nature, ethylene is produced in higher plants by 1-aminocyclopropane-1-carboxylic acid oxidase (ACCO). Biocatalytic alternatives for ethylene production are still far from being competitive
Neha Singhal et al.
The Biochemical journal, 461(2), 189-203 (2014-08-07)
Malaria parasites must respond to stresses and environmental signals to perpetuate efficiently during their multistage development in diverse environments. To gain insights into the parasite's stress response mechanisms, we investigated a conserved Plasmodium protein, which we have named plasmoDJ1 on
Hsueh-Fen Chen et al.
PloS one, 10(6), e0129903-e0129903 (2015-06-19)
Candida albicans is a major human fungal pathogen. One of the important features of C. albicans pathogenicity is the ability to form biofilms on mucosal surfaces and indwelling medical devices. Biofilm formation involves complex processes in C. albicans, including cell
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