from Horseradish P2075
Reagents Required but Not Provided
Methanol 494437
Acetic Acid, Glacial A6283
Ultrapure Water (18 Mcm or equivalent)
Precautions and Disclaimer
This product is for
Carnoy’s fixative [75%
methanol (Sigma Product No. M3641) : 25% Acetic Acid
(Sigma Product No. A6283)]. Let cultures stand for 2
minutes.
5. Aspirate the fluid off the dishes and gently add 2
of Carnoy’s
fixative [75% methanol (Product No. M3641) :
25% Glacial Acetic Acid, (Product No. A6283)]
to each culture and invert tubes to distribute
evenly.
9. Let the cultures sit for 5 minutes
Concentrate, Catalog
Number T4904
• Methanol, Catalog Number 179957
• Acetic Acid, Catalog Number A6283
• Brilliant Blue R Concentrate, Catalog Number
B8647 Or EZBlue Gel Staining Reagent, Catalog
Concentrate, Catalog
Number T4904
• Methanol, Catalog Number 179957
• Acetic Acid, Catalog Number A6283
• Brilliant Blue R Concentrate, Catalog Number
B8647 Or EZBlue Gel Staining Reagent, Catalog
Concentrate, Catalog
Number T4904
• Methanol, Catalog Number 179957
• Acetic Acid, Catalog Number A6283
• Brilliant Blue R Concentrate, Catalog Number
B8647 Or EZBlue Gel Staining Reagent, Catalog
Concentrate, Catalog
Number T4904
• Methanol, Catalog Number 179957
• Acetic Acid, Catalog Number A6283
• Brilliant Blue R Concentrate, Catalog Number
B8647 Or EZBlue Gel Staining Reagent, Catalog
Concentrate, Catalog
Number T4904
• Methanol, Catalog Number 179957
• Acetic Acid, Catalog Number A6283
• Brilliant Blue R Concentrate, Catalog Number
B8647 Or EZBlue Gel Staining Reagent, Catalog
Concentrate, Catalog
Number T4904
• Methanol, Catalog Number 179957
• Acetic Acid, Catalog Number A6283
• Brilliant Blue R Concentrate, Catalog Number
B8647 Or EZBlue Gel Staining Reagent, Catalog
mg/L Brilliant Blue R
(Catalog Number B0149)
5% (v/v) glacial Acetic acid
(Catalog Number A6283)
30 g/L Trichloroacetic Acid
(Catalog Number T4885).
Dissolve by warming to 60 °C for 30 minutes