B2643

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Showing 1-17 of 17 results for "B2643" within Papers

Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

J J Leary et al.

Proceedings of the National Academy of Sciences of the United States of America, 80(13), 4045-4049 (1983-07-01)

Biotin-labelled DNA probes, prepared by nick-translation in the presence of biotinylated analogs of TTP, are hybridized to DNA or RNA immobilized on nitrocellulose filters. After removal of residual probe, the filters are incubated for 2--5 min with a preformed complex

Cross-species analysis of Fc engineered anti-Lewis-Y human IgG1 variants in human neonatal receptor transgenic mice reveal importance of S254 and Y436 in binding human neonatal Fc receptor.

Ingrid J G Burvenich et al.

mAbs, 8(4), 775-786 (2016-04-01)

IgG has a long half-life through engagement of its Fc region with the neonatal Fc receptor (FcRn). The FcRn binding site on IgG1 has been shown to contain I253 and H310 in the CH2 domain and H435 in the CH3

Mingxin Liu et al.

Journal of cellular physiology, 234(10), 18731-18747 (2019-04-07)

The timely regulation of inflammatory M1 macrophage polarization toward regenerative M2 macrophages suggests the possibility of immunotherapy after myocardial infarction (MI). C1q/TNF-related protein-9 (CTRP9) has anti-inflammatory effects and can ameliorate heart function in mice after long-term myocardial infarction. The role

Enhancement of immune cellular agglutination by use of an avidin-biotin system.

S M Costello et al.

Clinical chemistry, 25(9), 1572-1580 (1979-09-01)

Classically, one can increase the titer of an agglutinating or first antibody with an antiglobulin or second antibody. We have used an avidin-biotin system in place of antiglobulin to similarly extend the agglutination by an initial anticellular antibody. Erythrocytes were

Avidin binding of carboxyl-substituted biotin and analogues.

K Hofmann et al.

Biochemistry, 21(5), 978-984 (1982-03-02)

Proteinase K-sensitive disease-associated ovine prion protein revealed by conformation-dependent immunoassay.

Alana M Thackray et al.

The Biochemical journal, 401(2), 475-483 (2006-10-05)

PrPSc [abnormal disease-specific conformation of PrP (prion-related protein)] accumulates in prion-affected individuals in the form of amorphous aggregates. Limited proteolysis of PrPSc results in a protease-resistant core of PrPSc of molecular mass of 27-30 kDa (PrP27-30). Aggregated forms of PrP

Early detection of NSCLC with scFv selected against IgM autoantibody.

Tetyana Pedchenko et al.

PloS one, 8(4), e60934-e60934 (2013-04-16)

Survival of patients with lung cancer could be significantly prolonged should the disease be diagnosed early. Growing evidence indicates that the immune response in the form of autoantibodies to developing cancer is present before clinical presentation. We used a phage-displayed

Expression of CD28 and CD40 in human myeloma cells: a comparative study with normal plasma cells.

C Pellat-Deceunynck et al.

Blood, 84(8), 2597-2603 (1994-10-15)

CD28 and CD40 are important activation pathways for T and B lymphocytes, respectively. The aim of this study was to determine the phenotype of plasma cells (PCs) and the expression of these two molecules, CD28 and CD40. Therefore, we have

Sensitivity enhancement of surface plasmon resonance biosensing of small molecules.

John S Mitchell et al.

Analytical biochemistry, 343(1), 125-135 (2005-06-14)

Surface plasmon resonance (SPR) biosensor formats using gold nanoparticle or protein signal amplification for the sensitive assay of small molecules were developed using progesterone as a model compound. Progesterone was immobilized to a dextran surface in the Biacore biosensor through

Effect of magnetite nanoparticle agglomerates on the destruction of tumor spheroids using high intensity focused ultrasound.

Vincent H B Ho et al.

Ultrasound in medicine & biology, 37(1), 169-175 (2010-11-19)

Magnetite (Fe(3)O(4)) nanoparticle agglomerates have been shown to enhance the degree of inertial cavitation induced by high-intensity focused ultrasound (HIFU). To investigate the effect of these particles on the destruction of tumor spheroids using HIFU, HeLa spheroids were insonated in

In vitro immune modulation by antibodies coupled to tumour cells.

D Darling et al.

Gene therapy, 4(12), 1350-1360 (1998-02-24)

Modification of autologous tumour cells to express the immune costimulator B7.1 is a potential strategy for immunotherapy of cancer. Previously, this has involved introduction of genetic material into cells, in vitro culture, and confirmation of the protein product on the

Biotin sensing in Saccharomyces cerevisiae is mediated by a conserved DNA element and requires the activity of biotin-protein ligase.

Heike M Pirner et al.

The Journal of biological chemistry, 281(18), 12381-12389 (2006-03-15)

Biotin is a water-soluble vitamin that functions as a prosthetic group in carboxylation reactions. In addition to its role as a cofactor, biotin has multiple roles in gene regulation. We analyzed biotin effects on gene expression in the yeast Saccharomyces

ATM-dependent phosphorylation of Mdm2 on serine 395: role in p53 activation by DNA damage.

R Maya et al.

Genes & development, 15(9), 1067-1077 (2001-05-02)

The p53 tumor suppressor protein, a key regulator of cellular responses to genotoxic stress, is stabilized and activated after DNA damage. The rapid activation of p53 by ionizing radiation and radiomimetic agents is largely dependent on the ATM kinase. p53

Ligation of major histocompatability complex (MHC) class I molecules on human T cells induces cell death through PI-3 kinase-induced c-Jun NH2-terminal kinase activity: a novel apoptotic pathway distinct from Fas-induced apoptosis.

S Skov et al.

The Journal of cell biology, 139(6), 1523-1531 (1998-02-12)

Ligation of major histocompatability complex class I (MHC-I) molecules expressed on T cells leads to both growth arrest and apoptosis. The aim of the current study was to investigate the intracellular signal pathways that mediate these effects. MHC-I ligation of

Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.

Gwendoline Thiery et al.

Journal of the American Society for Mass Spectrometry, 23(10), 1689-1696 (2012-08-08)

Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each

Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors.

C Hughes et al.

Molecular therapy : the journal of the American Society of Gene Therapy, 3(4), 623-630 (2001-04-26)

Three strategies have been designed to concentrate infectious retroviral vectors from the supernatants of human- (HT1080) and murine- (NIH 3T3) based packaging cells. Streptavidin-conjugated paramagnetic particles in conjunction with (i) antibodies directed against murine fibronectin, (ii) biotinylated lectins, or (iii)

Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein.

Emiliano Pavoni et al.

BMC cancer, 6, 41-41 (2006-03-01)

CEA is a tumor-associated antigen abundantly expressed on several cancer types, including those naturally refractory to chemotherapy. The selection and characterization of human anti-CEA single-chain antibody fragments (scFv) is a first step toward the construction of new anticancer monoclonal antibodies

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