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Showing 1-30 of 101 results for "C6137" within Papers
Jarrod R Fortwendel et al.
Antimicrobial agents and chemotherapy, 54(4), 1555-1563 (2010-02-04)
Attenuated activity of echinocandin antifungals at high concentrations, known as the "paradoxical effect," is a well-established phenomenon in Candida albicans and Aspergillus fumigatus. In the yeast C. albicans, upregulation of chitin biosynthesis via the protein kinase C (PKC), high-osmolarity glycerol
Wen-Jia Yang et al.
International journal of molecular sciences, 14(10), 20048-20063 (2013-10-12)
Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis. The cDNA
Krisztian Twaruschek et al.
Frontiers in microbiology, 9, 1954-1954 (2018-09-28)
In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the same strain (e.g., to test for redundant gene function) is a difficult task due to the limited availability of reliable selection markers. We have created
Suman Kumar Halder et al.
Journal of bioscience and bioengineering, 117(2), 170-177 (2013-09-03)
Production and optimization of β-N-acetyl glucosaminidase and chitinase by Ca-alginate immobilized Aeromonas hydrophila SBK1 was carried out using prawn shell as cost-effective substrate. Beads prepared with 5.0% Na-alginate (containing 2.0% colloidal chitin) and 1.0 M CaCl2 showed considerable beads integrity and
Michelino Di Rosa et al.
Molecular and cellular biochemistry, 374(1-2), 73-80 (2012-11-07)
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed
Jogi Madhuprakash et al.
The Journal of biological chemistry, 287(53), 44619-44627 (2012-11-02)
We describe the improvement of transglycosylation (TG) by chitinase D from Serratia proteamaculans (SpChiD). The SpChiD produced a smaller quantity of TG products for up to 90 min with 2 mm chitotetraose as the substrate and subsequently produced only hydrolytic
Lingling Zhang et al.
Journal of economic entomology, 107(2) (2014-04-30)
In addition to being used increasingly as a model system in modern molecular biology studies, the free-living nematode Caenorhabditis elegans (Maupas, 1900) is an important pathogen in fungi and straw mushrooms. In this study, Bacillus thuringiensis strain 010 was found
Lanelle R Connolly et al.
Methods in molecular biology (Clifton, N.J.), 1848, 235-263 (2018-09-06)
The bacteriophage P1 Cre/lox system has been utilized in diverse fungi for marker recycling and exchange, generation of targeted chromosome translocations, and targeted deletion of interstitial chromosome segments. Here we show the application of this tool in the wheat and
Vasiliki Skiada et al.
The New phytologist, 226(5), 1429-1444 (2020-01-31)
Legumes interact with a wide range of microbes in their root systems, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so-called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in
Krzysztof Nowacki et al.
Marine drugs, 18(6) (2020-06-06)
The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks
Ye Pan et al.
Journal of insect science (Online), 12, 150-150 (2013-03-07)
In insects, chitinases participate in the periodic shedding of old exoskeletons and the turnover of peritrophic membranes. Chitinase family members have been identified in dozens of species, including Tribolium castaneum, Drosophila melanogaster, and Anopheles gambiae. In this study, nine chitinases
Fei Liu et al.
Fungal genetics and biology : FG & B, 136, 103320-103320 (2019-12-22)
Leptosphaeria maculans is the causal agent of blackleg disease on Brassica napus. Determining the underlying functions of genes required for pathogenesis is essential for understanding the infection process. A chitin-binding protein (LmCBP1) was discovered as a pathogenicity factor for the
WeiJuan Yang et al.
Biosensors & bioelectronics, 41, 820-826 (2012-11-06)
As one of the most destructive and widespread disease of rice, Magnaporthe grisea (M. grisea) has a significant negative impact on rice production. Therefore, it is significant to develop a method for sensitive and high-throughput detection of M. grisea in
Hiroshi Nishino et al.
Cell and tissue research, 377(2), 193-214 (2019-03-05)
Vertebrates and insects are phylogenetically separated by millions of years but have commonly developed tympanal membranes for efficiently converting airborne sound to mechanical oscillation in hearing. The tympanal organ of the field cricket Gryllus bimaculatus, spanning 200 μm, is one of
Allergen bronchoprovocation and chitinases in allergic asthma.
G M Gauvreau
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 43(2), 149-151 (2013-01-22)
A-to-I mRNA editing controls spore death induced by a fungal meiotic drive gene in homologous and heterologous expression systems.
Lohmar, et al.
Genetics, 221 (2022)
José M Rodríguez-Peña et al.
FEBS letters, 587(22), 3675-3680 (2013-10-09)
Yeast adaptation to conditions in which cell wall integrity is compromised mainly relies on the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Zymolyase, a mixture of cell wall-digesting enzymes, triggers a peculiar signaling mechanism in which activation of
Jolanda M van Munster et al.
Microbiology (Reading, England), 159(Pt 9), 1853-1867 (2013-07-09)
Sporulation is an essential part of the life cycle of the industrially important filamentous fungus Aspergillus niger. The formation of conidiophores, spore-bearing structures, requires remodelling of the fungal cell wall, as demonstrated by the differences in carbohydrate composition of cell
Hongyin Zhang et al.
Carbohydrate polymers, 98(1), 366-371 (2013-08-31)
The influence of adding burdock fructooligosaccharide (BFO) in the culture media on the efficacy of Rhodotorula mucilaginosa in controlling postharvest decay of peaches and its possible mode of action were investigated. The antagonistic activity of R. mucilaginosa to Rhizopus decay
Yongjun Tang et al.
Journal of nanoscience and nanotechnology, 12(10), 8081-8086 (2013-02-21)
The chitinase producing bacterium H3 strain was screened from nature with the selective medium and was identified as Bacillus thuringiensis. The purpose of this investigation is to study the antifungi activity of Bacillus thuringiensis H3 chitinase and its immobilized particles
Xinyu Liu et al.
Bio-protocol, 7(5), e2164-e2164 (2017-03-05)
Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as
Frank Hoster et al.
Applied microbiology and biotechnology, 66(4), 434-442 (2004-08-04)
Thirteen different chitin-degrading bacteria were isolated from soil and sediment samples. Five of these strains (SGE2, SGE4, SSL3, MG1, and MG3) exhibited antifungal activity against phytopathogenic fungi. Analyses of the 16S rRNA genes and the substrate spectra revealed that the
Bart Oud et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(45), E4223-E4231 (2013-10-23)
Laboratory evolution of the yeast Saccharomyces cerevisiae in bioreactor batch cultures yielded variants that grow as multicellular, fast-sedimenting clusters. Knowledge of the molecular basis of this phenomenon may contribute to the understanding of natural evolution of multicellularity and to manipulating
Modular structure of HEL protein from Arabidopsis reveals new potential functions for PR-4 proteins.
Laura Bertini et al.
Biological chemistry, 393(12), 1533-1546 (2013-03-06)
Plants possess an innate immune system enabling them to defend themselves against pathogen attack.The accumulation of newly synthesized pathogenesis related proteins (PRs) is one of the most studied inducible plant defence response. In this paper, we report on the characterization
Melissa S Hillwig et al.
Journal of plant physiology, 168(7), 734-738 (2010-11-26)
Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To
Anna M Kielak et al.
Applied and environmental microbiology, 79(1), 263-272 (2012-10-30)
Chitin amendment is a promising soil management strategy that may enhance the suppressiveness of soil toward plant pathogens. However, we understand very little of the effects of added chitin, including the putative successions that take place in the degradative process.
Clemens J Heilmann et al.
Eukaryotic cell, 12(2), 254-264 (2012-12-18)
The human fungal pathogen Candida albicans can grow at temperatures of up to 45°C. Here, we show that at 42°C substantially less biomass was formed than at 37°C. The cells also became more sensitive to wall-perturbing compounds, and the wall
Tomoyasu Hirose et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(40), 15892-15897 (2013-09-18)
The Huisgen cycloaddition of azides and alkynes, accelerated by target biomolecules, termed "in situ click chemistry," has been successfully exploited to discover highly potent enzyme inhibitors. We have previously reported a specific Serratia marcescens chitinase B (SmChiB)-templated syn-triazole inhibitor generated
Shannon Quek et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(15), e2120003119-e2120003119 (2022-04-05)
Lymphatic filariasis is a vector-borne neglected tropical disease prioritized for global elimination. The filarial nematodes that cause the disease host a symbiotic bacterium, Wolbachia, which has been targeted using antibiotics, leading to cessation of parasite embryogenesis, waning of circulating larvae
Christine Klinger et al.
Marine drugs, 17(2) (2019-03-01)
Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain
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