gained interest for vaccine
clarification because they are significantly
easier to scale-up and implement.
NFF: Primary clarification using NFF
typically involves depth filters that often
contain positively
single-use clarification train
• Fully scalable from bench to process scale in a
modular, self-contained pod format
Clarification Solutions for Pre-treated
Feed Streams
Clarisolve® Depth Filters
pDADMAC
Celpure P Clarification Media
Figure 1 Celpure® P Clarification Media
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membranes make them ideal for sample
clarification prior to LC/MS.
MultiScreen® Solvinert Filter Plates are the perfect choice for automated sample clarification. To
accommodate use with aqueous and
selection of a primary clarification
filter. Several filter chemistries were evaluated,
including Polygard® CN50 (SN50A47FF3) and CN1H
(SN1HA47FF3) filters. However, the Polygard® filters
were not further
feed on traditional filteraid and cellulose based
clarification depth filters (Millistak+® HC)
2 Untreated feed on novel synthetic depth filters
(Millistak+® HC Pro)
3 Pretreated feed, using
synthetic depth filters was evaluated in the primary and
secondary clarification of CHO cell harvests and compared to
commercial benchmark devices.
Conclusion
1. Synthetic depth filters demonstrate a
consumables in the clarif ication
step (11).
Improper optimization of
clarification steps can affect the
performance of subsequent
downstream unit operations in terms
of capacity of filters or life of
ro
Primary clarification. In some cases, can be used as a single stage clarification step
going directly into a sterile filter.
C0HC
C0SP (synthetic)
Primary (coarse) clarification CE
Primary
User Guide
Millisolve™ Clarification System
Cat. No. XX1614700
Millisolve™ Clarification System2
Introduction
The Millisolve™ Clarification System is designed for vacuum filtration of
between Milligard® PES and Millipore Express® SHC filters.
Approach to Clarification - depends on
volume processed
≤1000 L volume: clarification using multi-
stage depth filtration offers cost savings
options would not solve the problem of
clarification filter filterability of the 2000L. A reduction in bioreactor size to 1000L, which would
allow for clarification, was not feasible due to the amount of
system
Innovative, High-Performance Pod Filters are Ideal for Primary and Secondary
Clarification in Lab, Pilot and Process-scale Applications
Millistak+® depth filter media is offered
in a scalable, disposable
Fully synthetic depth filters for clarification and downstream filtration applications.
Millistak+® HC Pro (high capacity synthetic
media) is a family of synthetic depth filters
providing a cleaner
inorganic filter aid DE25 to 75
Triple-layer A1HC Post-TFF (Prostak™) clarification fluids Tightest media combination with an
additional membrane layer to protect
downstream membrane filters
DE60
Pod disposable depth filter system or Millistak+®
HC filter, B1HC /X0HC media or Polygard®-CR filters
• Mobius® FlexReady solution for clarification
• Customized clarification systems
• EMPROVE® bio
performance not seen with traditional primary
clarification depth filters.
High-density cell culture processes pose a major challenge when it comes to
clarification and purification operations. Their high
disposable
depth filters are ideal for
primary and secondary clarification
in lab, pilot and process-scale applications
3
Table 1. pmax results for filter
Process-Scale Pod depth filters. In order
to minimize observed cell settling within process
tubing lines during primary clarification tests, specific
recommendations for filter tubing selection to minimize
and filtered through a diatomaceous earth-based filter
5 Wine after clarification, subjected to cold treatment
and filtered through a tangential polymeric filter
6 Wine after clarification, subjected
single-use clarification train
• Fully scalable from bench to process scale in a
modular, self-contained pod format
Clarification Solutions for
Pre-treated Feed Streams
Clarisolve® Depth Filters
pDADMAC
Millistak+® Pod Disposable Depth Filters
Millistak+® CE, DE, and HC Pod ideally is suited for primary and secondary clarification. These
disposable depth filters offer flexibility and ease of use through
Millistak+® Pod disposable depth filters which are ideally
suited for primary and secondary clarification.
The Mobius FlexReady Solution for Clarification
consists of single-use Flexware
pre-treatment solution when used in
combination with our Clarisolve® depth
filters. The Clarisolve® depth filter is a primary
clarification tool with a gradient density
structure specifically designed to the
spring
Clarification Prefilitration CarbonationStorage tank Final filtration Bottling Machine
reduce waste and save resources. Merck Millipore offers a
complete portfolio of filters and systems
support your clarification needs.
It features our innovative, high-performance
Millistak+® Pod disposable depth filters which are
ideally suited for primary and secondary clarification
and capable
Depth Filter Clarification Effects
on Feedstream Characteristics
Structure and Composition of Depth Filters
Included in the Millistak+® family of primary and sec-
ondary clarification filters are
times, provides for a
robust clarification platform. Additionally, utilizing Clarisolve®
depth filters for direct harvest, allows for a more compact
clarification train, through the compression