concentrating zone hptlc plates

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Showing 1-30 of 99 results for "concentrating zone hptlc plates" within Papers

Same analytical method for both (bio)assay and zone isolation to identify/quantify bioactive compounds by quantitative nuclear magnetic resonance spectroscopy.

Ebrahim Azadniya et al.

Journal of chromatography. A, 1628, 461434-461434 (2020-08-22)

Differing sensitivity is the main obstacle for a direct combination of HPTLC with NMR spectroscopy. A sufficient amount of the isolated compound zone must be provided by HPTLC for subsequent offline NMR detection (HPTLC//NMR). To fill the gap, a straightforward

Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology.

Andreas Schoenborn et al.

Journal of chromatography. A, 1530, 185-191 (2017-11-18)

The planar yeast estrogen screen (p-YES) can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. The method combines the

Determination of dialkyl phosphates as breakdown products of organophosphorus insecticides in fruit juices by HPTLC with fluorescence detection.

Wolfgang Schwack et al.

Journal of AOAC International, 92(3), 691-698 (2009-07-21)

Dialkyl phosphates (DAP) are common degradation products of organophosphorus pesticides that are used as urinary biomarkers for human exposure. An HPTLC method was developed for the quantitative determination of DAP in fruit juices, i.e., dimethyl phosphate (DMP), dimethyl thiophosphate (DMTP)

Development and validation of an HPTLC method for simultaneous quantitation of isoorientin, isovitexin, orientin, and vitexin in bamboo-leaf flavonoids.

Jin Wang et al.

Journal of AOAC International, 93(5), 1376-1383 (2010-12-15)

A simple HPTLC method has been developed for the simultaneous determination of isoorientin, isovitexin, orientin, and vitexin, both pure and in commercial samples of bamboo-leaf flavonoids. The flavone C-glycosides, including isoorientin, isovitexin, orientin, and vitexin, were extracted from bamboo-leaf flavonoids

Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry- mass spectrometry.

Carmen Jarne et al.

Journal of chromatography. A, 1638, 461895-461895 (2021-01-22)

Identification of 19 molecular species of globotriaosylceramides (Gb3) in extracts from a Fabry's plasma patient and a healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry and online coupling to Mass Spectrometry (MS). Separation was carried out on LiChrospher plates

Rolling--a new application technique for luminescent bacteria on high-performance thin-layer chromatography plates.

Vera Baumgartner et al.

Journal of chromatography. A, 1218(19), 2692-2699 (2011-02-19)

High-performance thin-layer chromatography (HPTLC) coupled with bioluminescence detection using Vibrio fischeri bacteria can be used for screening for unknown substances. This is accomplished by dipping the HPTLC plate in an aqueous bacteria solution. Especially polar substances, however, can start to

Morphological modification of nanostructured ultrathin-layer chromatography stationary phases.

S R Jim et al.

Journal of chromatography. A, 1218(40), 7203-7210 (2011-09-09)

Ultrathin-layer chromatography (UTLC) provides the high sensitivities and rapid separations over short distances desirable in many analytical applications. The dependence of these performance benefits on UTLC layer microstructure motivates continued stationary phase engineering efforts. A new method of modifying the

Off-line multidimensional high performance thin-layer chromatography for fractionation of Japanese knotweed rhizome bark extract and isolation of flavan-3-ols, proanthocyanidins and anthraquinones.

Urška Jug et al.

Journal of chromatography. A, 1637, 461802-461802 (2021-01-01)

A methodology based on off-line multidimensional thin-layer chromatography was developed for isolation of several secondary metabolites from bark of Japanese knotweed (Fallopia japonica Houtt.) rhizomes. Successive fractionation steps using PLC silica gel and HPTLC silica gel or HPTLC cellulose plates

Sharp-bounded zones link to the effect in planar chromatography-bioassay-mass spectrometry.

Ines Klingelhöfer et al.

Journal of chromatography. A, 1360, 288-295 (2014-08-26)

The traditional direct bioautography workflow was substantially altered to yield narrow, sharp-bounded effective zones. For the first time, microorganisms quantitatively detected the single effective compounds in complex samples, separated in parallel on a planar chromatogram. This novel effect-directed workflow was

High-performance thin-layer chromatography screening of multi class antibiotics in animal food by bioluminescent bioautography and electrospray ionization mass spectrometry.

Yisheng Chen et al.

Journal of chromatography. A, 1356, 249-257 (2014-07-13)

The world-wide usage and partly abuse of veterinary antibiotics resulted in a pressing need to control residues in animal-derived foods. Large-scale screening for residues of antibiotics is typically performed by microbial agar diffusion tests. This work employing high-performance thin-layer chromatography

Imaging high-performance thin-layer chromatography as powerful tool to visualize metabolite profiles of eight Bacillus candidates upon cultivation and growth behavior.

Stefanie Kruse et al.

Journal of chromatography. A, 1640, 461929-461929 (2021-02-21)

Imaging high-performance thin-layer chromatography (HPTLC) was explored with regard to its ability to visualize changes in the metabolite profile of bacteria. Bacillus subtilis has become a model organism in many fields. The increasing interest in these bacteria is driven by

HPTLC Bioautography Guided Isolation of α-Glucosidase Inhibiting Compounds from Justicia secunda Vahl (Acanthaceae).

Barbara A Theiler et al.

Phytochemical analysis : PCA, 28(2), 87-92 (2016-12-03)

α-Glucosidase inhibitors form an essential basis for the development of novel drugs in diabetes type 2 treatment. Searching for α-glucosidase inhibitors in plants, TLC bioautographic assays have been established and improved within the last years. In traditional medicine, extracts from

Non-targeted detection and differentiation of agonists versus antagonists, directly in bioprofiles of everyday products.

Ines Klingelhöfer et al.

Analytica chimica acta, 1125, 288-298 (2020-07-18)

Xenoestrogens exert antiandrogenic effects on the human androgen receptor. In the analytical field, such antagonists block the detection of testosterone and falsify results obtained by sum parameter assays. Currently, such agonistic versus antagonistic effects are not differentiated in complex mixtures.

Development and validation of a visible absorption densitometry method for quantitation of conessine in Holarrhena antidysenterica (Kurchi).

Rakesh K Patel et al.

Journal of AOAC International, 91(2), 339-343 (2008-05-15)

A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been proposed for the analysis of conessine in Holarrhena antidysenterica. The method involves visible densitometric evaluation of conessine resolving it by HPTLC on aluminium-based silica gel plates. For visible

Determination of 2-Aminoacetophenone in wine by high-performance thin-layer chromatography-fluorescence detection.

Nora Horlacher et al.

Journal of chromatography. A, 1432, 140-144 (2016-01-19)

2-Aminoacetophenone (AAP) is closely correlated with the appearance of the sensory phenomenon of UTA ("untypical aging off-flavor") in wine. AAP analyses are generally performed by gas chromatography and mass selective detection (GC/MS), when AAP is extracted from wines by liquid-liquid

Validation of HPTLC method for the analysis of taraxerol in Clitoria ternatea.

Venkatesan Kumar et al.

Phytochemical analysis : PCA, 19(3), 244-250 (2007-11-13)

A new, simple, sensitive, selective and precise HPTLC method has been developed for the determination of taraxerol in Clitoria ternatea L. Determination of taraxerol was performed on TLC aluminium plates. Linear ascending development was carried out in twin trough glass

Novel Chromatographic Methods for Simultaneous Quantification of Fish and Wheat Germ Oils Mixture in Pharmaceutical Dosage Forms.

Amira F El-Yazbi et al.

Journal of chromatographic science, 55(5), 497-507 (2017-02-17)

Two simple, direct and environment-friendly chromatographic methods, high-performance liquid chromatography (HPLC) and high-performance thin layer chromatographic (HPTLC), were developed for the determination of a binary mixture of fish oil (FO) and wheat germ oil (WGO), for the first time, in

High-performance thin-layer chromatographic determination of potato glycoalkaloids.

B Simonovska et al.

Journal of chromatography. A, 903(1-2), 219-225 (2001-01-12)

A high-performance thin-layer chromatographic (HPTLC) method for the determination of the two main potato glycoalkaloids, alpha-chaconine and alpha-solanine, in different parts of the potato plant, is described. Samples were extracted with diluted aqueous acetic acid containing the ion-pairing reagent 1-pentanesulfonic

Development and validation of an HPTLC method for apigenin 7-O-glucoside in chamomile flowers and its application for fingerprint discrimination of chamomile-like materials.

Etil Guzelmeric et al.

Journal of pharmaceutical and biomedical analysis, 107, 108-118 (2015-01-13)

Brewed tea of chamomile flowers (Matricaria recutita L.) (Asteraceae) has been extensively consumed for centuries due to either its pleasant taste or medicinal purposes. On the other hand, the major problem is difficulty in distinguishing the genuine specimen when supplying

Affordable and rapid HPTLC method for the simultaneous analysis of artemisinin and its metabolite artemisinic acid in Artemisia annua L.

Shazia Khan et al.

Biomedical chromatography : BMC, 29(10), 1594-1603 (2015-04-02)

Artemisinin (AN) and artemisinic acid (AA), valuable phyto-pharmaceutical molecules, are well known anti-malarials, but their activities against diseases like cancer, schistosomiasis, HIV, hepatitis-B and leishmaniasis are also being reported. For the simultaneous estimation of AN and AA in the callus

High-performance thin layer chromatography method for estimation of conessine in herbal extract and pharmaceutical dosage formulations.

Aman D Kaur et al.

Journal of pharmaceutical and biomedical analysis, 46(2), 391-394 (2007-11-17)

A new, simple, sensitive, precise and robust high-performance thin layer chromatographic (HPTLC) method was developed for the estimation of conessine in herbal extracts and pharmaceutical dosage forms. Analysis of conessine was performed on TLC aluminium plates pre-coated with silica gel

Simultaneous HPTLC analysis of ursolic acid, betulinic acid, stigmasterol and lupeol for the identification of four medicinal plants commonly available in the Indian market as Shankhpushpi.

Neeraj Kumar Sethiya et al.

Journal of chromatographic science, 53(5), 816-823 (2014-09-14)

In this study, we investigated a new, simple, sensitive, selective and precise high-performance thin-layer chromatography (HPTLC) fingerprint and quantitative estimation method for the analysis of ursolic acid, betulinic acid, stigmasterol and lupeol in Shankhpushpi botanicals. Linear ascending development was carried

Simultaneous Quantification of Gymnemic Acid as Gymnemagenin and Charantin as β-Sitosterol Using Validated HPTLC Densitometric Method.

Javed Ahamad et al.

Journal of chromatographic science, 53(7), 1203-1209 (2014-12-20)

Gymnemic acid and charantin are well-established antidiabetic phytosterols found in Gymnema sylvestre and Momordica charantia, respectively. The fact that these plants are often used together in antidiabetic poly-herbal formulations lured us to develop an HPTLC densitometric method for the simultaneous

Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging.

Snezana Agatonovic-Kustrin et al.

Journal of pharmaceutical and biomedical analysis, 102, 93-99 (2014-09-26)

Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined

Hedgehog activation promotes osteogenic fates of growth plate resting zone chondrocytes through transient clonal competency.

Shion Orikasa et al.

JCI insight, 9(2) (2023-12-06)

The resting zone of the postnatal growth plate is organized by slow-cycling chondrocytes expressing parathyroid hormone-related protein (PTHrP), which include a subgroup of skeletal stem cells that contribute to the formation of columnar chondrocytes. The PTHrP-Indian hedgehog feedback regulation is

Effect of radiation sterilization on alprenolol in the solid state studied by high-performance thin-layer chromatography.

Barbara Marciniec et al.

Journal of AOAC International, 93(3), 792-797 (2010-07-16)

The possibility of radiation sterilization of alprenolol (AL) has been studied. Irradiation of AL in solid form with a 25 kGy beam of electrons caused only an insignificant change in color that became more intense with increasing irradiation dose. Moreover

Four distinct chondrocyte populations in the fetal bovine growth plate: highest expression levels of PTH/PTHrP receptor, Indian hedgehog, and MMP-13 in hypertrophic chondrocytes and their suppression by PTH (1-34) and PTHrP (1-40).

Jürgen Weisser et al.

Experimental cell research, 279(1), 1-13 (2002-09-06)

Differentiation and growth of chondrocytes in fetal growth plates of vertebrate long bones and ribs appear to occur in a gradual, continuous manner between the resting zone through the proliferation zone, maturation zone, and upper and lower hypertrophic zones, with

New incorporation of the S9 metabolizing system into methods for detecting acetylcholinesterase inhibition.

Ebrahim Azadniya et al.

Analytica chimica acta, 1129, 76-84 (2020-09-07)

The activity of individual biotransformation products cannot be measured in multicomponent mixtures by the current status-quo assays. A prior separation and tedious isolation of compounds is required, and often in addition, a concentration step into a solvent suitable for the

Leptin regulates chondrocyte differentiation and matrix maturation during endochondral ossification.

Yuki Kishida et al.

Bone, 37(5), 607-621 (2005-07-26)

Leptin has been suggested to mediate a variety of actions, including bone development, via its ubiquitously expressed receptor (Ob-Rb). In this study, we investigated the role of leptin in endochondral ossification at the growth plate. The growth plates of wild-type

TLC densitometric quantification of picrosides (picroside-I and picroside-II) in Picrorhiza kurroa and its substitute Picrorhiza scrophulariiflora and their antioxidant studies.

Shashi Shankar Tiwari et al.

Biomedical chromatography : BMC, 26(1), 61-68 (2011-03-18)

Picroside-I and picroside-II are known bioactive metabolites in Picrorhiza species. In the present study a simple, precise method has been established for the simultaneous determination of picrosides (picroside-I and picroside-II) in two different Picrorhiza species, P. kurroa and P. scrophulariiflora.

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