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Showing 1-30 of 130 results for "D1626" within Papers
Shane W Rogers et al.
EcoHealth, 15(2), 409-425 (2018-03-11)
The purpose of this study was to investigate genetic biomarkers of zoonotic enteric pathogens and antibiotic-resistant genes (ARGs) in the feces of white-tailed deer (Odocoileus virginianus) as related to proximity of deer to land that receives livestock manure or human
Corey Frazer et al.
Current protocols in microbiology, 53(1), e76-e76 (2019-02-13)
Candida albicans is an opportunistic human fungal pathogen that is able to cause both mucosal and systemic infections. It is also a frequent human commensal, where it is typically found inhabiting multiple niches including the gastrointestinal tract. One of the
Siva Wu et al.
Pathogens and disease, 72(3), 143-160 (2014-06-20)
Nontypeable Haemophilus influenzae (NTHi), a human respiratory tract pathogen, can form colony biofilms in vitro. Bacterial cells and the amorphous extracellular matrix (ECM) constituting the biofilm can be separated using sonication. The ECM from 24- and 96-h NTHi biofilms contained
Arkadiy K Golov et al.
Scientific reports, 10(1), 15491-15491 (2020-09-25)
Large-scale epigenomic projects have mapped hundreds of thousands of potential regulatory sites in the human genome, but only a small proportion of these elements are proximal to transcription start sites. It is believed that the majority of these sequences are
Khaled Amara et al.
Bio-protocol, 9(17), e3347-e3347 (2019-09-05)
We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from single B cells of your
Joseph Hufton et al.
Physical chemistry chemical physics : PCCP, 18(42), 29101-29112 (2016-10-27)
Bacterial extra polymeric substances (EPS) have been associated with the extracellular precipitation of uranium. Here we report findings on the biomineralisation of uranium, with extracellular DNA (eDNA) used as a model biomolecule representative of EPS. The complexation and precipitation of
Jin H Song et al.
Cell chemical biology, 26(8), 1110-1121 (2019-06-04)
Increased telomerase activity is associated with malignancy and poor prognosis in human cancer, but the development of targeted agents has not yet provided clinical benefit. Here we report that, instead of targeting the telomerase enzyme directly, small molecules that bind
Stephen E Von Stetina et al.
Molecular biology of the cell, 28(15), 2042-2065 (2017-05-26)
To establish the animal body plan, embryos link the external epidermis to the internal digestive tract. In Caenorhabditis elegans, this linkage is achieved by the arcade cells, which form an epithelial bridge between the foregut and epidermis, but little is known
Yuji Matsukawa et al.
Colloids and surfaces. B, Biointerfaces, 172, 684-689 (2018-09-23)
We detected antioxidant activity of catechin, one of the main components of tea, using SWNTs surface coated with two different biomolecules. Compared to coating with DNA already reported, it can hardly be detected when coated with carboxymethyl cellulose. For nanobiosensing
Rachael M Giersch et al.
Bio-protocol, 7(18) (2017-10-31)
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that recruits the complex to a corresponding genomic
Robert Mans et al.
FEMS yeast research, 18(7) (2018-06-04)
Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for
Zhen Tong et al.
ACS chemical biology, 12(1), 300-310 (2016-12-21)
Mammalian SIRT7 is a member of the sirtuin family that regulates multiple biological processes including genome stability, metabolic pathways, stress responses, and tumorigenesis. SIRT7 has been shown to be important for ribosome biogenesis and transcriptional regulation. SIRT7 knockout mice exhibit
Gunnar F Kwakye et al.
Current protocols in toxicology, Chapter 12, Unit12-Unit12 (2011-05-10)
Cellular manganese (Mn) uptake and transport dynamics can be measured using a cellular fura-2 manganese extraction assay (CFMEA). The assay described here uses immortalized murine striatal cell line and primary cortical astrocytes, but the method is equally adaptable to other
Hewawasam Patuwatha Badathuruge Kalindu Dulanja Ramyasoma et al.
RNA biology, 17(7), 918-929 (2020-03-07)
Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever and dengue haemorrhagic fever or dengue
Huanhuan Hao et al.
Bio-protocol, 7(14), e2415-e2415 (2017-07-20)
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9) systems have emerged as a powerful tool for genome editing in many organisms. The wide use of CRISPR/Cas9 systems may be due to the fact that these systems contain a simple
Benjamin Clémençon et al.
Journal of pharmacological and toxicological methods, 92, 67-76 (2018-03-28)
Membrane proteins represent roughly one third of the human proteome and many of them serve as targets of therapeutic drugs. An exception is the SLC solute carrier superfamily with only a handful of approved drugs targeting SLCs. Indeed, for many
Marie-Louise Ainalem et al.
Langmuir : the ACS journal of surfaces and colloids, 26(7), 4965-4976 (2009-12-05)
This study shows that DNA (linearized plasmid, 4331 base pairs and salmon sperm, 2000 base pairs, respectively) adsorbs to model membranes of zwitterionic liquid crystalline phospholipid bilayers in solutions containing divalent Ca(2+) cations, and also in solutions containing monovalent Na(+).
Yuji Matsukawa et al.
Molecules (Basel, Switzerland), 26(4) (2021-03-07)
Semiconductor single-walled carbon nanotubes (SWNTs) have unique characteristics owing to differences in the three-dimensional structure (chirality) expressed by the chiral index (n,m), and many studies on the redox characteristics of chirality have been reported. In this study, we investigated the
S Siefring et al.
Journal of water and health, 6(2), 225-237 (2008-01-23)
Previously reported and redesigned primer and probe assays were evaluated for the quantitative analysis of the fecal indicator bacterial groups, Enterococcus and Bacteroidetes with three real-time PCR instrument and reagent systems. The efficiency and sensitivity of the original assays varied
Emi Haladjova et al.
Macromolecular bioscience, 21(2), e2000352-e2000352 (2020-12-08)
Physicochemical characteristics and biological performance of polyplexes based on two identical copolymers bearing tertiary amino or quaternary ammonium groups are evaluated and compared. Poly(2-(dimethylamino)ethyl methacrylate)-b-poly(oligo(ethylene glycol) methyl ether methacrylate) block copolymer (PDMAEMA-b-POEGMA) is synthesized by reversible addition fragmentation chain transfer
Xin Jiang et al.
Translational neurodegeneration, 7, 24-24 (2018-10-20)
Circadian rhythms are oscillating physiological and behavioral changes governed by an internal molecular clock, and dysfunctions in circadian rhythms have been associated with ageing and various neurodegenerative diseases. However, the evidence directly connecting the neurodegeneration-associated proteins to circadian control at
Lumeng Ye et al.
PloS one, 9(11), e110038-e110038 (2014-11-05)
Pseudomonas putida is a member of the fluorescent pseudomonads known to produce the yellow-green fluorescent pyoverdine siderophore. P. putida W15Oct28, isolated from a stream in Brussels, was found to produce compound(s) with antimicrobial activity against the opportunistic pathogens Staphylococcus aureus
Bharat V Bhut et al.
Biotechnology and bioengineering, 108(11), 2654-2660 (2011-05-28)
The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the
Erhan Aslan et al.
European journal of protistology, 51(2), 173-185 (2015-04-10)
Deoxyribonucleases (DNases) play a major role in apoptotic DNA fragmentation/degradation, and apoptotic-like DNA degradation is also observed during conjugation of the ciliate Tetrahymena thermophila; however, the characteristics of neutral and acidic DNases are still undefined in its life stages. Here
Marc M Baum et al.
BMC microbiology, 9, 103-103 (2009-05-23)
Microbial biofilms represent an incompletely understood, but fundamental mode of bacterial growth. These sessile communities typically consist of stratified, morphologically-distinct layers of extracellular material, where numerous metabolic processes occur simultaneously in close proximity. Limited reports on environmental isolates have revealed
Lindsay B Avery et al.
mAbs, 10(2), 244-255 (2017-12-23)
Implementation of in vitro assays that correlate with in vivo human pharmacokinetics (PK) would provide desirable preclinical tools for the early selection of therapeutic monoclonal antibody (mAb) candidates with minimal non-target-related PK risk. Use of these tools minimizes the likelihood
Andree Schmidt et al.
Journal of leukocyte biology, 81(1), 186-194 (2006-10-21)
Alveolar macrophages (AMs) and peribronchial/perivascular macrophages are probably involved in lung allograft damage. We investigate leukocyte infiltration into graft tissue and address the question whether proliferation in situ contributes to macrophage homeostasis and accumulation. Lung transplantation was performed in the
Sharad Gupta et al.
The Journal of biological chemistry, 287(13), 9982-9989 (2011-12-22)
Huntington disease (HD) is one of several fatal neurodegenerative disorders associated with misfolded proteins. Here, we report a novel method for the sensitive detection of misfolded huntingtin (HTT) isolated from the brains of transgenic (Tg) mouse models of HD and
Annemarie H Becker et al.
Nature protocols, 8(11), 2212-2239 (2013-10-19)
A plethora of factors is involved in the maturation of newly synthesized proteins, including chaperones, membrane targeting factors and enzymes. Many factors act co-translationally through association with ribosome-nascent chain complexes (RNCs), but their target specificities and modes of action remain
Shannon Owens et al.
Methods in enzymology, 601, 275-307 (2018-03-11)
Homologous recombination is fundamental to sexual reproduction, facilitating accurate segregation of homologous chromosomes at the first division of meiosis, and creating novel allele combinations that fuel evolution. Following initiation of meiotic recombination by programmed DNA double-strand breaks (DSBs), homologous pairing
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