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Showing 1-30 of 130 results for "D1626" within Papers
Gunnar F Kwakye et al.
Current protocols in toxicology, Chapter 12, Unit12-Unit12 (2011-05-10)
Cellular manganese (Mn) uptake and transport dynamics can be measured using a cellular fura-2 manganese extraction assay (CFMEA). The assay described here uses immortalized murine striatal cell line and primary cortical astrocytes, but the method is equally adaptable to other
Stephen E Von Stetina et al.
Molecular biology of the cell, 28(15), 2042-2065 (2017-05-26)
To establish the animal body plan, embryos link the external epidermis to the internal digestive tract. In Caenorhabditis elegans, this linkage is achieved by the arcade cells, which form an epithelial bridge between the foregut and epidermis, but little is known
Huanhuan Hao et al.
Bio-protocol, 7(14), e2415-e2415 (2017-07-20)
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9) systems have emerged as a powerful tool for genome editing in many organisms. The wide use of CRISPR/Cas9 systems may be due to the fact that these systems contain a simple
Benjamin Clémençon et al.
Journal of pharmacological and toxicological methods, 92, 67-76 (2018-03-28)
Membrane proteins represent roughly one third of the human proteome and many of them serve as targets of therapeutic drugs. An exception is the SLC solute carrier superfamily with only a handful of approved drugs targeting SLCs. Indeed, for many
Hewawasam Patuwatha Badathuruge Kalindu Dulanja Ramyasoma et al.
RNA biology, 17(7), 918-929 (2020-03-07)
Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever and dengue haemorrhagic fever or dengue
Bharat V Bhut et al.
Biotechnology and bioengineering, 108(11), 2654-2660 (2011-05-28)
The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the
Yuji Matsukawa et al.
Molecules (Basel, Switzerland), 26(4) (2021-03-07)
Semiconductor single-walled carbon nanotubes (SWNTs) have unique characteristics owing to differences in the three-dimensional structure (chirality) expressed by the chiral index (n,m), and many studies on the redox characteristics of chirality have been reported. In this study, we investigated the
Emi Haladjova et al.
Macromolecular bioscience, 21(2), e2000352-e2000352 (2020-12-08)
Physicochemical characteristics and biological performance of polyplexes based on two identical copolymers bearing tertiary amino or quaternary ammonium groups are evaluated and compared. Poly(2-(dimethylamino)ethyl methacrylate)-b-poly(oligo(ethylene glycol) methyl ether methacrylate) block copolymer (PDMAEMA-b-POEGMA) is synthesized by reversible addition fragmentation chain transfer
Corey Frazer et al.
Current protocols in microbiology, 53(1), e76-e76 (2019-02-13)
Candida albicans is an opportunistic human fungal pathogen that is able to cause both mucosal and systemic infections. It is also a frequent human commensal, where it is typically found inhabiting multiple niches including the gastrointestinal tract. One of the
Joseph Hufton et al.
Physical chemistry chemical physics : PCCP, 18(42), 29101-29112 (2016-10-27)
Bacterial extra polymeric substances (EPS) have been associated with the extracellular precipitation of uranium. Here we report findings on the biomineralisation of uranium, with extracellular DNA (eDNA) used as a model biomolecule representative of EPS. The complexation and precipitation of
Rachael M Giersch et al.
Bio-protocol, 7(18) (2017-10-31)
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that recruits the complex to a corresponding genomic
Xin Jiang et al.
Translational neurodegeneration, 7, 24-24 (2018-10-20)
Circadian rhythms are oscillating physiological and behavioral changes governed by an internal molecular clock, and dysfunctions in circadian rhythms have been associated with ageing and various neurodegenerative diseases. However, the evidence directly connecting the neurodegeneration-associated proteins to circadian control at
Lumeng Ye et al.
PloS one, 9(11), e110038-e110038 (2014-11-05)
Pseudomonas putida is a member of the fluorescent pseudomonads known to produce the yellow-green fluorescent pyoverdine siderophore. P. putida W15Oct28, isolated from a stream in Brussels, was found to produce compound(s) with antimicrobial activity against the opportunistic pathogens Staphylococcus aureus
Tam D Vo et al.
Physical chemistry chemical physics : PCCP, 23(24), 13490-13502 (2021-06-14)
Electrostatic protein/DNA interactions arise from the neutralization of the DNA phosphodiester backbone as well as coupled exchanges by charged protein residues as salt bridges or with mobile ions. Much focus has been and continues to be paid to interfacial ion
Nicklas Heine Staunstrup et al.
Disease models & mechanisms, 10(7), 869-880 (2017-07-07)
Psoriasis is a complex human-specific disease characterized by perturbed keratinocyte proliferation and a pro-inflammatory environment in the skin. Porcine skin architecture and immunity are very similar to that in humans, rendering the pig a suitable animal model for studying the
Zhen Tong et al.
ACS chemical biology, 12(1), 300-310 (2016-12-21)
Mammalian SIRT7 is a member of the sirtuin family that regulates multiple biological processes including genome stability, metabolic pathways, stress responses, and tumorigenesis. SIRT7 has been shown to be important for ribosome biogenesis and transcriptional regulation. SIRT7 knockout mice exhibit
Yuji Matsukawa et al.
Colloids and surfaces. B, Biointerfaces, 172, 684-689 (2018-09-23)
We detected antioxidant activity of catechin, one of the main components of tea, using SWNTs surface coated with two different biomolecules. Compared to coating with DNA already reported, it can hardly be detected when coated with carboxymethyl cellulose. For nanobiosensing
Elisabete Ms Castanheira et al.
Nanoscale research letters, 6(1), 379-379 (2011-06-30)
Fluorescence properties of two new potential antitumoral tetracyclic thieno[3,2-b]pyridine derivatives were studied in solution and in liposomes of DPPC (dipalmitoyl phosphatidylcholine), egg lecithin (phosphatidylcholine from egg yolk; Egg-PC) and DODAB (dioctadecyldimethylammonium bromide). Compound 1, pyrido[2',3':3,2]thieno[4,5-d]pyrido[1,2-a]pyrimidin-6-one, exhibits reasonably high fluorescence quantum
Robert Mans et al.
FEMS yeast research, 18(7) (2018-06-04)
Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for
S Siefring et al.
Journal of water and health, 6(2), 225-237 (2008-01-23)
Previously reported and redesigned primer and probe assays were evaluated for the quantitative analysis of the fecal indicator bacterial groups, Enterococcus and Bacteroidetes with three real-time PCR instrument and reagent systems. The efficiency and sensitivity of the original assays varied
Marie-Louise Ainalem et al.
Langmuir : the ACS journal of surfaces and colloids, 26(7), 4965-4976 (2009-12-05)
This study shows that DNA (linearized plasmid, 4331 base pairs and salmon sperm, 2000 base pairs, respectively) adsorbs to model membranes of zwitterionic liquid crystalline phospholipid bilayers in solutions containing divalent Ca(2+) cations, and also in solutions containing monovalent Na(+).
Tam D Vo et al.
Analytical biochemistry, 629, 114298-114298 (2021-07-13)
To extend the value of biosensor-SPR in the characterization of DNA recognition by nucleoproteins, we report a comparative analysis of DNA-facilitated target search by two ETS-family transcription factors: Elk1 and ETV6. ETS domains represent an attractive system for developing biosensor-based
Annemarie H Becker et al.
Nature protocols, 8(11), 2212-2239 (2013-10-19)
A plethora of factors is involved in the maturation of newly synthesized proteins, including chaperones, membrane targeting factors and enzymes. Many factors act co-translationally through association with ribosome-nascent chain complexes (RNCs), but their target specificities and modes of action remain
Y Miura et al.
The British journal of nutrition, 67(2), 257-265 (1992-03-01)
Effects of quantity and quality of dietary proteins on plasma immunoreactive insulin-like growth factor-1 (IGF-1) concentration, and content of IGF-1 mRNA in rat liver were investigated in rats. Plasma immunoreactive IGF-1 concentration in rats given a casein diet was higher
Joanna Kud et al.
Bio-protocol, 9(18), e3372-e3372 (2019-09-20)
In this study, we describe a standard whole mount in situ hybridization method which is used to determine the spatial-temporal expression pattern of genes from Globodera spp. Unlike more invasive radioactive labeling approaches, this technique is based on a safe
Tuan Anh Pham et al.
Current genetics, 57(4), 233-239 (2011-05-13)
Transformation is an indispensable method for the genetic manipulation of cells. Saccharomyces cerevisiae can be transformed by incubating intact cells and plasmid DNA in the presence of polyethylene glycol alone. Lithium acetate (LiAc) and single-stranded carrier DNA (ssDNA) enhance the
Drake A Donovan et al.
Cell reports, 29(8), 2520-2535 (2019-11-21)
Regulation of chromatin structure is essential for controlling access of DNA to factors that require association with specific DNA sequences. Here we describe the development and validation of engineered chromatin remodeling proteins (E-ChRPs) for inducing programmable changes in nucleosome positioning by
Emi Haladjova et al.
Macromolecular bioscience, 18(4), e1700349-e1700349 (2018-03-01)
The present contribution is focused on feasibility of using comb-like copolymers of polyethylenimine with poly(2-ethyl-2-oxazoline) (LPEI-comb-PEtOx) with varying grafting densities and degrees of polymerization of PEI and PEtOx to deliver DNA molecules into cells. The copolymers form small and well-defined
Masayo Naito et al.
The American journal of pathology, 174(1), 54-62 (2008-12-20)
Acute kidney injury evokes renal tubular cholesterol synthesis. However, the factors during acute kidney injury that regulate HMG CoA reductase (HMGCR) activity, the rate-limiting step in cholesterol synthesis, have not been defined. To investigate these factors, mice were subjected to
Mariafrancesca Hyeraci et al.
ChemMedChem, 15(15), 1464-1472 (2020-06-04)
Platinum(II) complexes of the type [Pt(Cl)(PPh3 ){(κ2 -N,O)-(1{C(R)=N(OH)-2(O)C6 H4 })}] with R=Me, H, (1 and 2) were synthesized and characterized. Single-crystal X-ray diffraction confirmed the proposed (SP4-3) configuration for 1. Study of the antiproliferative activity, performed on a panel of
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