Search Within
D8537
Applied Filters:
Keyword:'D8537'
Showing 1-30 of 329 results for "D8537" within Papers
Melissa Santi et al.
ACS biomaterials science & engineering, 6(9), 4862-4869 (2021-01-05)
As a first approach, standard 2D cell culture techniques are usually employed for the screening of drugs and nanomaterials. Despite the easy handling, findings achieved on 2D cultures are often not efficiently translatable to in vivo preclinical investigations. Furthermore, although
Emma K Jennings et al.
STAR protocols, 2(1), 100284-100284 (2021-02-04)
This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how
Carl W White et al.
Cell chemical biology, 27(5), 499-510 (2020-02-14)
G protein-coupled receptors are a major class of membrane receptors that mediate physiological and pathophysiological cellular signaling. Many aspects of receptor activation and signaling can be investigated using genetically encoded luminescent fusion proteins. However, the use of these biosensors in
Magdalena Meyer et al.
International journal of molecular sciences, 21(6) (2020-03-27)
The neuropeptide oxytocin (OT) is a well-described modulator of socio-emotional traits, such as anxiety, stress, social behavior, and pair bonding. However, when dysregulated, it is associated with adverse psychiatric traits, such as various aspects of autism spectrum disorder (ASD). In
Xinyue Li et al.
mBio, 12(2) (2021-04-08)
At the intestinal host-microbe interface, the transmembrane mucin MUC1 can function as a physical barrier as well as a receptor for bacteria. MUC1 also influences epithelial cell morphology and receptor function. Various bacterial pathogens can exploit integrins to infect eukaryotic
Lidia Węglińska et al.
Cells, 10(5) (2021-05-06)
Congenital and acquired toxoplasmosis caused by the food- and water-born parasite Toxoplasma gondii (T. gondii) is one of the most prevalent zoonotic infection of global importance. T. gondii is an obligate intracellular parasite with limited capacity for extracellular survival, thus
Mirza Muhammad Fahd Qadir et al.
Nature communications, 11(1), 3265-3265 (2020-07-01)
The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested
Asier Ullate-Agote et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(42), 26307-26317 (2020-10-07)
Reptiles exhibit a spectacular diversity of skin colors and patterns brought about by the interactions among three chromatophore types: black melanophores with melanin-packed melanosomes, red and yellow xanthophores with pteridine- and/or carotenoid-containing vesicles, and iridophores filled with light-reflecting platelets generating
Diane G Edmondson et al.
Current protocols, 1(2), e44-e44 (2021-02-19)
For over a century, investigation of Treponema pallidum subsp. pallidum, the spiral-shaped bacterium that causes syphilis, was hindered by an inability to culture the organism in vitro. A recent breakthrough has enabled continuous in vitro growth of this organism in
Elisabeth Müller et al.
Frontiers in immunology, 8, 1383-1383 (2017-11-11)
Tumor-associated macrophages may either promote or suppress tumor growth depending on their activation status. Interferon-γ (IFN-γ) has been identified as a key factor for inducing tumoricidal M1 phenotype in macrophages. However, it remains unclear whether IFN-γ is sufficient or if
Steven Fiering et al.
eLife, 4, e04796-e04796 (2015-07-17)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replicating selected results from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and
Carlos M Martínez-Viturro et al.
Journal of medicinal chemistry, 63(22), 14017-14044 (2020-11-17)
O-GlcNAcylation is a post-translational modification of tau understood to lower the speed and yield of its aggregation, a pathological hallmark of Alzheimer's disease (AD). O-GlcNAcase (OGA) is the only enzyme that removes O-linked N-acetyl-d-glucosamine (O-GlcNAc) from target proteins. Therefore, inhibition
Ahmed Sadik et al.
Cell, 182(5), 1252-1270 (2020-08-21)
Aryl hydrocarbon receptor (AHR) activation by tryptophan (Trp) catabolites enhances tumor malignancy and suppresses anti-tumor immunity. The context specificity of AHR target genes has so far impeded systematic investigation of AHR activity and its upstream enzymes across human cancers. A
Trese Leinders-Zufall et al.
STAR protocols, 2(2), 100527-100527 (2021-05-25)
Small molecular probes designed for photopharmacology and opto-chemogenetics are rapidly gaining widespread recognition for investigations of transient receptor potential canonical (TRPC) channels. This protocol describes the use of three photoswitchable diacylglycerol analogs-PhoDAG-1, PhoDAG-3, and OptoDArG-for ultrarapid activation and deactivation of
M Wang et al.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 72(2) (2021-08-11)
The present study was performed to examine the protective effects of rhamnetin against the development of atherosclerosis and its effects on the regulation of several pathways. The anti-atherosclerosis effect of rhamnetin was examined in cells stimulated with oxidized low-density lipoprotein
M Cardoso Dos Santos et al.
Bioconjugate chemistry, 29(4), 1327-1334 (2018-02-21)
Fluorescent nanoparticles (NPs) have become irreplaceable tools for advanced cellular and subcellular imaging. While very bright NPs require excitation with UV or visible light, which can create strong autofluorescence of biological components, NIR-excitable NPs without autofluorescence issues exhibit much lower
Zhuzhen Zhang et al.
Nature medicine, 24(5), 617-627 (2018-04-18)
Proliferating cells, compared with quiescent cells, are more dependent on glucose for their growth. Although glucose transport in keratinocytes is mediated largely by the Glut1 facilitative transporter, we found that keratinocyte-specific ablation of Glut1 did not compromise mouse skin development
Ruli Gao et al.
Nature biotechnology, 39(5), 599-608 (2021-01-20)
Single-cell transcriptomic analysis is widely used to study human tumors. However, it remains challenging to distinguish normal cell types in the tumor microenvironment from malignant cells and to resolve clonal substructure within the tumor. To address these challenges, we developed
Regina Wirth et al.
Bio-protocol, 10(9), e3603-e3603 (2021-03-05)
Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of RNA. Although a handful of light-up aptamers
Xinyue Li et al.
PLoS pathogens, 15(2), e1007566-e1007566 (2019-02-05)
The cellular invasion machinery of the enteric pathogen Salmonella consists of a type III secretion system (T3SS) with injectable virulence factors that induce uptake by macropinocytosis. Salmonella invasion at the apical surface of intestinal epithelial cells is inefficient, presumably because
L T Daum et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 18(7), 847-849 (2014-06-07)
In many parts of the world, the diagnosis of tuberculosis (TB) has rapidly shifted to molecular detection and sequencing formats. The collection and transport of Mycobacterium tuberculosis specimens thus remains a challenging problem where TB is common and the infrastructure
Rodrigo Diéguez-Hurtado et al.
Nature communications, 10(1), 2817-2817 (2019-06-30)
Sufficient vascular supply is indispensable for brain development and function, whereas dysfunctional blood vessels are associated with human diseases such as vascular malformations, stroke or neurodegeneration. Pericytes are capillary-associated mesenchymal cells that limit vascular permeability and protect the brain by
Emile Alghoul et al.
STAR protocols, 2(3), 100677-100677 (2021-08-12)
Inducible biomolecular condensates play fundamental roles in cellular responses to intracellular and environmental cues. Knowledge about their composition is crucial to understand the functions that arise specifically from the assembly of condensates. This protocol combines an optogenetic and an efficient
Ernesto Cortes et al.
Hepatology (Baltimore, Md.), 69(2), 785-802 (2018-07-29)
Hepatic stellate cells (HSCs) are essential perisinusoidal cells in both healthy and diseased liver. HSCs modulate extracellular matrix (ECM) homeostasis when quiescent, but in liver fibrosis, HSCs become activated and promote excess deposition of ECM molecules and tissue stiffening via
Satomi Hirose et al.
Cell adhesion & migration, 15(1), 272-284 (2021-09-23)
The collective migration of vascular endothelial cells plays important roles in homeostasis and angiogenesis. Oxygen concentration in vivo, which is lower than in the atmosphere and changes due to diseases, is a key factor affecting the cellular dynamics of vascular
Hisham F Bahmad et al.
Journal of personalized medicine, 10(3) (2020-08-14)
Prostate cancer (PC) is the most frequently diagnosed cancer among men worldwide. The poor prognosis of PC is largely due to late diagnosis of the disease when it has progressed to advanced stages marked by androgen-independence. We interrogated proteomic signatures
Enikő Balogh et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(6), 1088-1099 (2019-05-10)
Objective- Vascular calcification is associated with high risk of cardiovascular events and mortality. Osteochondrogenic differentiation of vascular smooth muscle cells (VSMCs) is the major cellular mechanism underlying vascular calcification. Because tissue hypoxia is a common denominator in vascular calcification, we
O Sascha Yousefi et al.
Bio-protocol, 10(5), e3540-e3540 (2021-03-05)
T cells are one major cell type of the immune system that use their T cell antigen receptor (TCR) to bind and respond to foreign molecules derived from pathogens. The ligand-TCR interaction half-lives determine stimulation outcome. Until recently, scientists relied
Nadège Bossuet-Greif et al.
Bio-protocol, 7(16), e2520-e2520 (2017-08-20)
Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA damages, ultimately leading to cell cycle arrest, senescence and death. Here we describe a simple method
Luca Scaccini et al.
International journal of molecular sciences, 22(15) (2021-08-08)
Peripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration
Page 1 of 11