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eppendorf centrifuges
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Product Information Sheet - SC1000
in
the media needing to be removed by centrifugation.
SigmaPrep is a trademark of Sigma-Aldrich Co. LLC
Eppendorf is a registered trademark of Eppendorf AG
Product Information Sheet - CYTO1A
teflon and glass tissue grinder.
2.3 Centrifuge the homogenate at 7,250 x g at 4 °C for
30 minutes. (Eppendorf centrifuge Model 5415 C
at 9,500 rpm using 2 ml Eppendorf tubes)
2.4 Carefully remove and
Product Information Sheet - C3228
Sigma-Aldrich Biotechnology LP.
Coomassie is a registered trademark of ICI PLC.
Eppendorf is a registered trademark of Eppendorf-
Netheler-Hinz GmbH.
JWM/KMR/MAM 11/03
Sigma brand
Eppendorf MiniSpin (MiniSpin +)
Microsoft Word - Eppendorf minispin.doc
Eppendorf MiniSpin (MiniSpin +)
Z605220 and Z606235
* Rotor 5452 000 018 is included with the MiniSpin and 5453 000 011 with MiniSpin +
Product Information Sheet - C3228
Sigma-Aldrich Biotechnology LP.
Coomassie is a registered trademark of ICI PLC.
Eppendorf is a registered trademark of Eppendorf-
Netheler-Hinz GmbH.
JWM/KMR/MAM 11/03
Sigma brand
Product Information Sheet - C4482
No. P8215
• Dithiothreitol (DTT), Product No. D9779
• Test tubes
• Shaker
• Centrifuge
• Microcentrifuge Eppendorf 5417R (Product
No. Z366013 or Z366021) or equivalent
Precautions and Disclaimer
Product Information Sheet - CYTO2B
teflon and glass tissue
grinder.
2.3) Centrifuge the homogenate in an
Eppendorf centrifuge at 4°C for 30 minutes
at 9,500 rpm (RCF ≅ 7,250xg) using 2 ml
Eppendorf tubes.
2.4) Carefully remove and save
Data Sheet - P3244
-heptane.
10. Vortex for 10 seconds and centrifuge at maximum
speed for 10 minutes, to separate the phases.
11. Prepare a set of 1.5 mL Eppendorf tubes containing
0.35 mg (0.00035 g) silica
Product Information Sheet - MITOISO1
Reagents and Equipment Required
(Not provided)
• Cooled Eppendorf centrifuge (for small scale),
or Sorvall RC-5C centrifuge with SS-34 head
(for large scale)
• PTFE pestle and 3 mL glass
Product Information Sheet - A1979
suspension. To equilibrate the resin, add
5 volumes of PBS, mix, and centrifuge for 30 seconds
at 2,000 x g (e.g., 5,000 rpm in a Eppendorf 5415 C
microcentrifuge). Carefully remove the supernatant
using
Product Information Sheet - MAK331
1.5 mL centrifuge
tube.
2. Add 20 L of Precipitation Reagent.
3. Vortex to mix and centrifuge 5 minutes at 9,500 g
(e.g., 9,500 rpm in an Eppendorf 5415C tabletop
centrifuge).
Product Information Sheet - BTAG
spectrophotometer
• Variable speed microcentrifuge or low speed
centrifuge (e.g., clinical centrifuge)
• Screw cap microcentrifuge tubes or flip-top
Eppendorf tubes (1.0–1.5 ml)
• 96 well plate (optional, for
Product Information Sheet - R1153
Standard
Solution to a small Eppendorf tube.
2. Add 5 µl of Denaturant Solution containing 2% octyl
β-D-glucopyranoside and 0.1 M 2-mercaptoethanol.
Mix and centrifuge briefly.
3. Incubate at
Troubleshooting guide for Eppendorf Minispin - Z605220 and Z60623
Word - Epp. Minispin Troubleshooting web page.doc
Troubleshooting guide for Eppendorf Minispin - Z605220 and Z606235
ERROR
Product Information Sheet - MAS007
minutes.
4. Centrifuge the Sample Tube for
5-10 minutes at full speed. Regain the
supernatant for assay (Dilution
Factor = 2).
Red Wine
1. Weigh 40 mg of PVPP powder in an
Eppendorf tube.
Product Information Sheet - E3403
a 1.5 ml microcentrifuge tube.
2. Immediately centrifuge lysate for 10 minutes at
8,200 × g in a microcentrifuge (e.g., 10,000 rpm in
an Eppendorf 5415C microcentrifuge) at 2−8 °C
to pellet cell
Product Information Sheet - MAK532
culture or
tissue culture treated plates are
not recommended.
• Eppendorf tubes and centrifuge
• 1.5 mL Centrifuge tubes
• heat block or hot water bath (optional)
Product Information Sheet - P6486
to a 1.5 ml microcentrifuge tube.
2. Immediately centrifuge lysate for 10 minutes at
8,200 × g in a microcentrifuge (10,000 rpm in an
Eppendorf 5415C microcentrifuge) at 2−8 °C to
pellet cell debris
Product Information Sheet - I0408
Standard Solution to a
small Eppendorf tube.
2. Add 5 µl of Denaturant Solution containing 2% octyl
β-D-glucopyranoside and 0.1 M 2-mercaptoethanol.
Mix and centrifuge briefly.
3. Incubate at 100
Product Information Sheet - E2404
of the Lysis Buffer to the tube, vortex, and
centrifuge in a microcentrifuge for approximately
30 seconds at 8,200 X g (e.g., 10,000 rpm in an
Eppendorf 5415C microcentrifuge). Carefully
remove the
HybriScan Detection System
Thermomixer Comfort, Eppendorf Z605271
Exchange unit for 24 x 2 mL reaction tubes; Eppendorf Z605670
Exchange unit for microtiter plate; Eppendorf T3942
Centrifuge for 2 mL reaction tubes Z605220
SimpliconTM Expression System: "Designing, Cloning and RNA Synthesis for Expression of Self-Replicative RNA"
Ambion AM9786). These could
include tabletop centrifuges, heat blocks, vortex, pipettes, tube racks, pipette racks and anything that
will touch the eppendorf tubes containing the RNA. Rinse with MilliQ
SimpliconTM Expression System: Designing Cloning and RNA Synthesis for Expression of Self-Replicative RNA
Ambion AM9786). These could
include tabletop centrifuges, heat blocks, vortex, pipettes, tube racks, pipette racks and anything that
will touch the eppendorf tubes containing the RNA. Rinse with MilliQ
Product Information Sheet - M0444
suspension. To equilibrate
the medium, add 5 volumes of PBS, mix, and centrifuge
for 30 seconds at 2,000 x g (e.g., 5,000 rpm in a
Eppendorf® 5415 C microcentrifuge). Carefully remove
the supernatant using
Product Information Sheet - CS0490
120
2
Reagents and Equipment Required But Not
Provided
• Microfuge centrifuge - Eppendorf® microcentrifuge
5415 Series (Product Code Z60,406-2) or
equivalent.
•
ADP-ribosylcyclase (A8950) - Enzyme Assay
Reagent A and determine the concentration using the extinction coefficient. Centrifuge
the remaining solution of C-7323 in an Eppendorf tube at high speed for 10 minutes in a
microcentrifuge. This solution
Product Information Sheet - PP0200
tweezers
• Siliconized Eppendorf tubes (Product Code
T 4691 or equivalent)
• 37 °C heating block or heating bath
• Scalpel (S 2771 and S 3021) or razor blade
• Bench-top centrifuge (microcentrifuge)
Data Sheet - MSKT0004
Barnstead Thermolyne
AROS 160 Adjustable Reciprocating Orbital
Shaker
• Thermomixer, such as Eppendorf Thermomixer C
• LC-MS/MS system, such as Sciex 5500
Precautions and Disclaimer
For R&D
Product Information Sheet - P9740
with 30% acetonitrile) to the tube.
Vortex, and centrifuge in a microcentrifuge for
30 seconds at 8,200 g (10,000 rpm in an
Eppendorf 5415C microcentrifuge).
Discard the
Technical Bulletin - PP0100
tweezers
• siliconized Eppendorf tubes (Product Code T 4691
or equivalent)
• 37 °C heating block or heating bath
• scalpel (S 2771 and S 3021) or razor blade
• bench-top centrifuge (microcentrifuge)
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