eppendorf centrifuges

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Preactivated Magnetic Beads for Affinity Chromatography

Covalent coupling of antibodies, aptamers, and proteins with a NHS Mag Sepharose, Sera-Mag Carboxylate-Modiﬁed, Sera-Mag SpeedBeads Carboxylate-Modiﬁed from Cytiva.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.

Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

Fractional Precipitation Protocol for Affinity Chromatography Samples

This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.

A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®

Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Tips & Tricks | Sample Handling and Preparation | MILLIPLEX^® Assays

Have questions about sample handling and preparation for MILLIPLEX® multiplex panels? Explore information on sample collection, sample handling, and sample preparation for multiplex assays with tips and tricks to get the most out of your assays.

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale puriﬁcation (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

MystiCq® MicroRNA® Quantitation System

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

Duolink® PLA Flow Cytometry Protocol

Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.

Mass Spectrometry of Glycans

The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Simplicon™ RNA Transfection and Electroporation Protocols

Viral RNA Extraction Buffer for SARS-CoV-2 in Saline and Saliva

Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.