Application Note
Surfactants (nonionic) in
Extran® rinse solutions
Sample preparation and photometric determination of nonionic surfactants in Extran®
rinse solutions (MA 01, MA 02, MA 05)
Introduction
Application Note
Surfactants (nonionic) in
Extran® rinse solutions
Sample preparation and photometric determination of nonionic surfactants in Extran®
rinse solutions (MA 01, MA 02, MA 05, AP 22)
treatment method for
animal-derived material that has been demonstrated to remove
up to 6 logs of extraneous agents. The process must be tightly
controlled in order to be consistent, reproducible, effective
404
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One of the critical aspects of designing an extraneous agent
inactivation validation is the selection of specific microbes to
evaluate. Those used in
remain at the plasma-Histopaque-1077 interface.
Erythrocyte contamination is negligible. Most
extraneous platelets are removed by low speed
centrifugation during the washing steps.
Product Information
at the upper
plasma/Histopaque-1077 interface. Erythrocyte
contamination is negligible. Most extraneous platelets
found in the upper interface are removed by low speed
centrifugation during washing
acetonitrile
performance of UHPLC Plus, for gradient elution grade vs. three
competitors. The lack of extraneous peaks along the baseline, in
conjunction with the lower baseline intensity after ~25 minutes
appropriate depth
and start the GC.
3. Run GC program until completed
4. There will typically be some extraneous peaks in the initial runs
5. Repeat the step again to see if there is a reduction in the size and
remain at the plasma/ Histopaque-1077
interface. Erythrocyte contamination is
negligible. Most extraneous platelets are
removed by low speed centrifugation during
the washing steps.
1. To a 15 mL
concentrations of
methanolic HCl reduce the time necessary for
complete reaction, but can create extraneous
byproducts that can interfere with the
analysis. Lower concentrations of methanolic
concentrations of
methanolic HCl reduce the time necessary for
complete reaction, but can create extraneous
byproducts that can interfere with the
analysis. 3 M hydrogen chloride solution
remains
chromatography of the derivative, but Tallent, et
al.,1 found that ammonium chloride can cause extraneous
peaks with products containing epoxide rings. Some
analysts separate the salt by allowing it
chromatography of the derivative, but Tallent, et
al.,2 found that ammonium chloride can cause extraneous
peaks with products containing epoxide rings. Some
analysts separate the salt by allowing it
pipette.
Erythrocyte contamination is avoided due to
the barrier between the chambers.
Most extraneous platelets are removed by
low speed centrifugation during the
washing steps.
1. Bring desired
band with the mononuclear cells
and will move towards the bottom of the centrifuge
tube. Most extraneous platelets are removed by low
speed centrifugation during the washing steps.
1. Add
pipette.
Erythrocyte contamination is avoided due to
the barrier between the chambers.
Most extraneous platelets are removed by
low speed centrifugation during the
washing steps.
1. Bring desired
pipette.
Erythrocyte contamination is avoided due to
the barrier between the chambers.
Most extraneous platelets are removed by
low speed centrifugation during the
washing steps.
1. Bring desired
film designated for chemiluminescent detection, such as Kodak
Biomax Light, MS, and MR.
Extraneous
spots
Aggregated protein or antibody
conjugate
Filter the conjugate through a 0.2 micron
entry into
production processes, Verlenden
stresses that it is essential to care-
fully monitor extraneous input,
as contaminants are invariably in-
troduced through outside sources.
“This means we have
centrifuge the
conjugate solution at 10,000 g for 10 minutes and use the supernatant.
Extraneous
spots
Too much antibody or protein
conjugate
Perform a titer of the antibody or protein
REAGENT SELECTION
In the course of IC experiments, we have been confronted
with the presence of extraneous peaks on the chro-
matograms. In searching for the source of the contamination,
it appeared that
Switch to film designated for chemiluminescent detection such as
BioMax® Light, MS, and MR.
Extraneous
spots
Aggregated protein or
antibody conjugate
Centrifuge the conjugate solution at 10,000
Negative Control
shows PCR
product (signal)
Reagents or reactions have
been contaminated
Extraneous DNA template may have been introduced into the reagents or
when setting up the PCR reactions.
Negative Control
shows PCR
product (signal)
Reagents or reactions have
been contaminated
Extraneous DNA template may have been introduced into the reagents or
when setting up the PCR reactions.
Negative Control
shows PCR
product (signal)
Reagents or reactions have
been contaminated
Extraneous DNA template may have been introduced into the reagents or
when setting up the PCR reactions.
Negative
Control shows
PCR product
(signal)
Reagents or reactions
have been contaminated
Extraneous DNA template may have been introduced into the
reagents or when setting up the PCR reactions.
Negative
Control shows
PCR product
(signal)
Reagents or reactions
have been contaminated
Extraneous DNA template may have been introduced into the
reagents or when setting up the PCR reactions.
Negative Control
shows PCR
product (signal)
Reagents or reactions
have been
contaminated
Extraneous DNA template may have been introduced into the reagents
or when setting up the PCR reactions.
Negative Control
shows PCR
product (signal)
Reagents or reactions
have been
contaminated
Extraneous DNA template may have been introduced into the reagents
or when setting up the PCR reactions.