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Showing 1-30 of 99 results for "F7252" within Papers
A C Dhar et al.
Stain technology, 57(3), 151-155 (1982-05-01)
Fast green FCF was used to localize acidic nuclear proteins in sections of young flower buds of Limnophyton obtusifolium (L.) Miq. After extracting nucleic acids, the slides were stained at hydrogen ion concentrations ranging from pH 2.6 to 9.0. At
Long Ma et al.
International journal of molecular medicine, 47(3) (2021-01-16)
The present study aimed to investigate whether dihydroartemisinin (DHA) alleviates osteoarthritis (OA) in a mouse model of OA. Ten‑week‑old female C57BL/6j mice were used to establish OA models by anterior cruciate ligament transection (ACLT) and ovariectomized (OVX). DHA was then
Lydia Wood et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(40), 12440-12448 (2009-10-09)
Rett syndrome, an autism spectrum disorder with prominent motor and cognitive features, results from mutations in the gene for methyl-CpG-binding protein 2 (MeCP2). Here, to identify cortical circuit abnormalities that are specifically associated with MeCP2 deficiency, we used glutamate uncaging
A M Thompson et al.
Molecular and cellular neurosciences, 109, 103554-103554 (2020-09-25)
The current study sought to characterize the pro-survival effects of erythropoietin (EPO) in a toxicant model of Parkinson's disease (PD). EPO treatment induced time-dependent elevations of antioxidant glutathione peroxidase (GPx) and anti-apoptotic factors (pAkt and pBad/Bad) within the striatum and
Aimy Sebastian et al.
Cells, 10(6) (2021-07-03)
Articular cartilage is a connective tissue lining the surfaces of synovial joints. When the cartilage severely wears down, it leads to osteoarthritis (OA), a debilitating disease that affects millions of people globally. The articular cartilage is composed of a dense
Tatyana A Meyers et al.
Journal of molecular and cellular cardiology, 128, 51-61 (2019-01-22)
Duchenne muscular dystrophy (DMD) is an X-linked disease characterized by skeletal muscle degeneration and a significant cardiomyopathy secondary to cardiomyocyte damage and myocardial loss. The molecular basis of DMD lies in the absence of the protein dystrophin, which plays critical
Miki Jishage et al.
Genes & development, 34(11-12), 767-784 (2020-05-10)
Liver regeneration and metabolism are highly interconnected. Here, we show that hepatocyte-specific ablation of RNA polymerase II (Pol II)-associated Gdown1 leads to down-regulation of highly expressed genes involved in plasma protein synthesis and metabolism, a concomitant cell cycle re-entry associated
Melanie E Mendez et al.
International journal of molecular sciences, 21(17) (2020-09-10)
Osteoarthritis (OA) is a painful and debilitating disease characterized by the chronic and progressive degradation of articular cartilage. Post-traumatic OA (PTOA) is a secondary form of OA that develops in ~50% of cases of severe articular injury. Inflammation and re-occurring
Paul A Muller et al.
Science (New York, N.Y.), 370(6514), 314-321 (2020-08-29)
The gut microbiota affects tissue physiology, metabolism, and function of both the immune and nervous systems. We found that intrinsic enteric-associated neurons (iEANs) in mice are functionally adapted to the intestinal segment they occupy; ileal and colonic neurons are more
Samantha R Weaver et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(5), 986-999 (2021-01-13)
Endochondral ossification is tightly controlled by a coordinated network of signaling cascades including parathyroid hormone (PTH). Pleckstrin homology (PH) domain and leucine rich repeat phosphatase 1 (Phlpp1) affects endochondral ossification by suppressing chondrocyte proliferation in the growth plate, longitudinal bone
Qi Sun et al.
eLife, 10 (2021-03-02)
Osteoarthritis, a highly prevalent degenerative joint disorder, is characterized by joint pain and disability. Available treatments fail to modify osteoarthritis progression and decrease joint pain effectively. Here, we show that intermittent parathyroid hormone (iPTH) attenuates osteoarthritis pain by inhibiting subchondral
Astrid Deryckere et al.
eLife, 10 (2021-08-25)
Cephalopods have evolved nervous systems that parallel the complexity of mammalian brains in terms of neuronal numbers and richness in behavioral output. How the cephalopod brain develops has only been described at the morphological level, and it remains unclear where
L B Gilmore et al.
Journal of biochemical and biophysical methods, 5(1), 57-66 (1981-07-01)
The quantitation of proteins in polyacrylamide gels stained with Fast green FCF has been investigated using a modification of the elution technique originally described by Fenner et al. (Fenner, C., Traut, R.R., Mason, D.T. and Wikman-Coffelt, J. (1975) Anal. Biochem.
Géraldine Meyer-Dilhet et al.
STAR protocols, 1(1), 100027-100027 (2020-07-21)
The cerebral cortex is composed of an exquisitely complex network of interconnected neurons supporting the higher cognitive functions of the brain. Here, we provide a fully detailed, step-by-step protocol to perform in utero cortical electroporation of plasmids, a simple surgical
Guoli Hu et al.
The American journal of pathology, 190(8), 1701-1712 (2020-05-18)
Interleukin 17A (IL-17A) is critical in the pathogenesis of autoimmune diseases through driving inflammatory cascades. However, the role of IL-17 in osteoarthritis (OA) is not well understood. Tumor necrosis factor-receptor-associated factor 3 (TRAF3) is a receptor proximal negative regulator of
Aimy Sebastian et al.
International journal of molecular sciences, 21(1) (2020-01-16)
Aging and injury are two major risk factors for osteoarthritis (OA). Yet, very little is known about how aging and injury interact and contribute to OA pathogenesis. In the present study, we examined age- and injury-related molecular changes in mouse
Victoria Rozés-Salvador et al.
Frontiers in cell and developmental biology, 8, 550267-550267 (2020-10-06)
Neural development is a complex process that involves critical events, including cytoskeleton dynamics and selective trafficking of proteins to defined cellular destinations. In this regard, Smad Anchor for Receptor Activation (SARA) is an early endosome resident protein, where perform trafficking-
Xiakun Yuan et al.
Environmental toxicology and pharmacology, 32(2), 242-248 (2011-08-17)
The objective of the study was to assess the effect of ciprofloxacin antibiotic on the physiological or phenotypic characteristics of food-borne toxigenic Fusarium graminearum and F. avenaceum molds under in vitro conditions. In the presence of ciprofloxacin, Fusarium mycelia growth
Collin Challis et al.
Nature neuroscience, 23(3), 327-336 (2020-02-19)
Parkinson's disease is a synucleinopathy that is characterized by motor dysfunction, death of midbrain dopaminergic neurons and accumulation of α-synuclein (α-Syn) aggregates. Evidence suggests that α-Syn aggregation can originate in peripheral tissues and progress to the brain via autonomic fibers.
Melanie E Mendez et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 35(11), 2229-2241 (2020-06-22)
Osteoarthritis (OA) is a debilitating and painful disease characterized by the progressive loss of articular cartilage. Post-traumatic osteoarthritis (PTOA) is an injury-induced type of OA that persists in an asymptomatic phase for years before it becomes diagnosed in ~50% of
Yuan Fang Li et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 18(2), 177-181 (2002-03-05)
The interaction of Fast Green FCF (FCF) with proteins (including bovine serum albumin (BSA), human serum albumin (HSA), pepsin (Pep) and alpha-chymotrypsin (Chy), and lysozyme (Lys)) was characterized by enhanced resonance light-scattering (RLS) measurements using a common spectrofluorometer. The enhanced
Veronica Ulici et al.
The American journal of pathology, 189(4), 868-885 (2019-01-22)
Mitogen-activated protein kinases, including c-Jun NH2-terminal kinase (JNK), play an important role in the development and function of a large variety of tissues. The skeletal phenotype of JNK1 and JNK2 double-knockout (dKO) mice (JNK1fl/flCol2-Cre/JNK2-/-) and control genotypes were analyzed at
Michael D Rainey et al.
Cell reports, 32(9), 108096-108096 (2020-09-03)
DNA replication initiates from multiple origins, and selective CDC7 kinase inhibitors (CDC7is) restrain cell proliferation by limiting origin firing. We have performed a CRISPR-Cas9 genome-wide screen to identify genes that, when lost, promote the proliferation of cells treated with sub-efficacious
João Carlos Gonçalves et al.
The Journal of cell biology, 218(3), 808-819 (2019-01-25)
Cytoplasmic dynein participates in multiple aspects of neocortical development. These include neural progenitor proliferation, morphogenesis, and neuronal migration. The cytoplasmic dynein light intermediate chains (LICs) 1 and 2 are cargo-binding subunits, though their relative roles are not well understood. Here
Masahiro Yasuda et al.
STAR protocols, 2(3), 100742-100742 (2021-08-26)
The visual system is the best system to study activity-dependent sensory circuit development. The connections from the retina to the dorsal lateral geniculate nucleus, the retinogeniculate connections, undergo extensive remodeling during early postnatal life. Thus, techniques that allow the expression
Fanling Meng et al.
Journal of molecular biology, 406(3), 491-502 (2011-01-05)
Islet amyloid polypeptide (IAPP; also known as amylin) is responsible for islet amyloid formation in type 2 diabetes, and IAPP-induced toxicity is believed to contribute to the loss of β-cell mass associated with the late stages of type 2 diabetes.
Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green.
Shen Luo et al.
Analytical biochemistry, 350(2), 233-238 (2005-12-13)
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this
R N Misra et al.
Mutation research, 170(1-2), 75-78 (1986-04-01)
Three widely used dyes, acridine orange, blue VRS and fast green FCF were administered to male mice in order to study the induction of gross chromosomal anomalies using the micronucleus test. All 3 compounds were shown to be clastogenic.
Yan Zhao et al.
Frontiers in cell and developmental biology, 8, 445-445 (2020-07-07)
The contributing factors and the origins of precursor cells in traumatic heterotopic ossification around the temporomandibular joint (THO-TMJ), which causes obvious restriction of mouth opening and maxillofacial malformation, remain unclear. In this study, our findings demonstrated that injured chondrocytes in
Minyi Qian et al.
Cellular and molecular gastroenterology and hepatology, 13(1), 233-256 (2021-08-29)
The ligand-activated transcription factor, aryl hydrocarbon receptor (AHR) can sense xenobiotics, dietary, microbial, and metabolic cues. Roles of Ahr in intestinal epithelial cells (IECs) have been much less elucidated compared with those in intestinal innate immune cells. Here, we explored
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