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Cell-based assays

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Forskolin-Induced Swelling Assays
A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.
Organoids Generation and Phenotypic Screen on Millicell® Microwell Plates
Discover how to use Millicell® Microwell plates to generate mouse intestinal and colorectal cancer organoids and develop a high throughput phenotypic drug development screen.
Preparation of Antibody Sensitized Sheep Erythrocytes
Protocol for the preparation of Antibody Sensitized Sheep Erythrocytes. Includes product numbers and links.
The Endothelial Cell Transwell Migration and Invasion Assay
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
T-Cell Migration Assays Using Millicell® Cell Culture Inserts
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
In Vitro T Cell Killing Assay Protocol
Millicell® Microwell plates facilitate T cell killing assays, evaluating killing capacity in tumor spheroids for personalized assays.
Cultrex® Basement Membrane Extract, Type 2, PathClear® Protocol
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.