Cytology and microscopy

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CsCl gradient centrifugation separates RNA from DNA; differential and density gradient centrifugation techniques explained.

细胞学取样_细胞学诊断_细胞学诊断的染色方法-默克生命科学

细胞学是研究细胞结构和功能的学科，而细胞学诊断则是细胞学在诊断上的应用。细胞学诊断利用显微镜并依靠完善、高度特异性的染色和实验方案来实现细胞异常鉴定。

Cell Migration & Invasion Assays

Study tumor metastasis via various migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay, and scratch assay.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Evolution of Cell Culture Surfaces

For good cell attachment the hydrophobic polystyrene surface must be modified to a more hydrophilic surface. This allows cell attachment proteins (vitronectin and fibronectin) found in the serum containing culture medium to adhere and spread on the vessel bottom providing

Primary Cell Culture Basics

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Water for Histology Staining

Reliable histology staining requires consistent quality pure water. See examples of GMS and H&E stains performed with various water contaminants and comparing tap vs Type 2 water.

Detergent-Free Nuclear Protein Extraction

Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

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