The ease of assembly of a given peptide sequence is hard to predict, which makes peptide synthesis challenging. Review methods and reagents for avoiding aggregation in solid-phase peptide synthesis.
Review methods and resins for attaching amino acids and peptides, including Merrifield, trityl-based, and hydroxymethyl-functionalized resins. Resin-immobilized peptides can be used for various downstream applications.
Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.
We provide an overview of our available reagents, together with recommendations and details of their use for synthesis of peptides containing post-translationally modified amino acids.
The most popular reagent for cleavage of peptides from Boc-based resins is anhydrous HF. Of all the cleavage procedures HF appears to be the most versatile and least harmful to a wide variety of peptides synthesized on Boc-based resins.
Fmoc resin cleavage and deprotection follows the difficult task of detaching the peptide from the resin support and removing all the side-chain protecting groups of the amino acid residues to yield the desired peptide.