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原代细胞培养_哺乳动物细胞_采购和培养原代细胞-默克生命科学
原代细胞是直接从存活人或动物组织收获的哺乳动物细胞。原代细胞与哺乳动物细胞系的不同之处在于，它们通常要求更为苛刻，需要提供额外的营养成分——特别是在低血清含量或无血清的培养基中培养时。这些额外的明确营养成分包括细胞因子和生长因子。
Cell Imaging with Fluorescent Lentiviral Biosensors
Fluorescent lentiviral particles encoding important GFP/RFP fusion proteins related to autophagy, apoptosis, and cell structure that enables live cell imaging.
ECM Coating Protocols for Millicell®
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
3D Mammosphere Culture Media
3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.
ADCC Assay Using BioIVT Cryopreserved NK Cells
ADCC assay performance and reproducibility using ready-to-use, cryopreserved NK effector cells, A549 target cells expressing a luciferase reporter gene, and anti-EGFR antibody.
Primary Airway Epithelial Cells
Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.
BL21 Chemically Competent Cells Overview
BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
Authenticated Breast Cancer Cell Lines for Cancer Research
Choose from more than 30 breast cancer cell lines authenticated by ECACC, classified by hormone receptor-positive, HER2-positive, and triple-negative cancers, as well as by known mutations such as BRCA2.
Primary Human Cardiac Myocytes and Myocyte Growth Media
Primary human cardiac myocytes isolated from normal human ventricle tissue of the adult heart, plus optimized myocyte media. Protocols for handling, cell passaging, media, and product use.
Primary Human Chondrocytes and Chondrocyte Growth Media
Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.
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