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Protein biology
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Showing 1-16 of 16 results
Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins
This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
Purification or Removal of Albumin
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel
This page shows how to purify histidine-tagged proteins secreted into eukaryotic
cell culture supernatants using Ni Sepharose Excel from Cytiva.
Enzymatic Assay of 5’-Nucleotidase
To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.
Enzymatic Assay & ATP Sensitivity Test of Luciferase
Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.
Dextran Sulfate
Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.
Assay Procedure for Xanthine Oxidase Microbial
The appearance of uric acid is measured at 293nm by spectrophotometry.
Enzymatic Assay of Trypsin Inhibitor
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
Column Packing and Preparation Protocol for Affinity Chromatography
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
Large volume concentration and diafiltration with Amicon® Stirred Cells
Demonstrating the effective use of Amicon Stirred Cell for large volume concentration through diafiltration.
Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
Rapid, Ultrafiltration-based Antibody Purification
Ultrafiltration-based MAb purification offers faster, higher recovery compared to hybridoma screening.