Protein mass spectrometry

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What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.

Universal Proteomics Standards – Validating the Future of Proteomics

The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined with advances in mass spectrometry, have decreased concerns of sample complexity, and

Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard

Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.

Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation

In this study, we developed a rapid trypsin digest kit that, at elevated temperatures, yielded reliable, reproducible results in less than 2 hours on a wide variety of substrates for mass spectrometry.

Three Methods for Critical Quality Attribute Determination of Monoclonal Antibodies

Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS

The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

Benefits of Ascentis Express Phenyl-Hexyl over Biphenyl for the Separation of Pain Management Opiates

Although both biphenyl and phenyl-hexyl phases can resolve these compounds, the former exhibits excellent peak shape and substantially less silanol-derived ion exchange activity.

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards

Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.

Ion Chromatography for Sugar Testing in Food and Environmental Analysis

In this article we demonstrate how well ion chromatography is suited as an analytical technique for sugar analysis e.g. in apple juice, milk, and dust samples.

Absolute Quantification of Serum Proteome

Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.

Quantification of Host Cell Proteins

Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.

Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components

Protein AQUA™ Applications

Utilize the protein AQUA™ technique to measure expression of silenced genes, quantitate protein levels for low abundance serum proteins and analyze phosphorylated peptides enriched with immobilized metal affinity chromatography.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.