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Large molecule HPLC
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facet applications:Large molecule HPLC
facet applications:Low pressure liquid chromatography
facet applications:Small molecule HPLC
facet content type:Technical Article
Showing 1-30 of 198 results
HPLC Columns for Antibody and Large Protein Analysis
BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.
HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan
BIOshell™ Glycan U/HPLC Columns
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the
Protein Affinity Chromatography
Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
Detection, Measurement and Regulation of NOGE (Novolac Glycidyl Ethers) and Other Packaging-Derived Epoxy Compounds In Food Products
Detection, Measurement and Regulation of NOGE (Novolac Glycidyl Ethers) and Other Packaging-Derived Epoxy Compounds In Food Products
UHPLC analysis of Benzoyl peroxide and degradation products on Purospher® STAR RP-18e Hibar® HR
UHPLC analysis of Benzoyl peroxide and degradation products on Purospher®STAR RP-18e Hibar® HR - Conditions & Description
Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction
Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.
Bioanalysis with SPME
Biocompatible SPME devices employ C18 bonded porous silica sorbent particles, in a binder which resists fouling by biological matrix components
Ascentis® Express Biphenyl UHPLC and HPLC Columns
The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the
Ascentis® Express C8 U/HPLC Columns
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators
Ion-Suppression & Phospholipid Contamination
We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
HPLC Analysis of Adenosine Monophosphate (AMP) and Adenosine Triphosphate (ATP) on SeQuant ZIC-HILIC
Separation of AMP (adenosine monophosphate); ATP (adenosine triphosphate)
Derivatization and Separation of Aliphatic Amines
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
Supel™-Select Polymeric SPE
Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.
Columns and Analytical Standards for Protein SEC
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
Setting Column Pressure Limits for Size Exclusion Chromatography
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS
Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.
Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Troubleshooting Guide for Affinity Chromatography of Tagged Proteins
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Troubleshooting Reversed Phase Chromatography (RPC)
Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Viral Clearance Using Capto™ Adhere
Description of viral clearance using Capto adhere from Cytiva.
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