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Showing 1-30 of 278 results for "G2133" within Papers
Vesa Havurinne et al.
eLife, 9 (2020-10-21)
Sacoglossan sea slugs are able to maintain functional chloroplasts inside their own cells, and mechanisms that allow preservation of the chloroplasts are unknown. We found that the slug Elysia timida induces changes to the photosynthetic light reactions of the chloroplasts
Juan Li et al.
Biosensors & bioelectronics, 41, 698-703 (2012-10-23)
Multi-walled carbon nanotubes (MWCNTs)-nanoflake-like SnS(2) nanocomposite were designed for immobilization of glucose oxidase (GOx). The direct electrochemistry of GOx and glucose sensing at MWCNTs-SnS(2) modified glassy carbon electrode were studied. Compared with single MWCNTs or SnS(2), the MWCNTs-SnS(2) film has
David J Schodt et al.
Biomedical optics express, 14(1), 429-440 (2023-01-27)
We describe a dedicated microscope for automated sequential localization microscopy which we term Sequential Super-resolution Microscope (SeqSRM). This microscope automates precise stage stabilization on the order of 5-10 nanometers and data acquisition of all user-selected cells on a coverslip, limiting
Mireia Andreu-Carbó et al.
STAR protocols, 3(2), 101320-101320 (2022-05-03)
Microtubules are dynamic polymers where tubulin exchanges not only at the ends but also all along the microtubule shaft. In vitro reconstitutions are a vital approach to study microtubule tip dynamics, while direct observation of shaft dynamics is challenging. Here, we
Muskan Floren et al.
Oncogene, 39(19), 3910-3925 (2020-03-24)
A principal challenge in treating acute myeloid leukemia (AML) is chemotherapy refractory disease. As such, there remains a critical need to identify key regulators of chemotherapy resistance in AML. In this study, we demonstrate that the membrane scaffold, CD82, contributes
Nora C Goossen-Schmidt et al.
Scientific reports, 10(1), 13746-13746 (2020-08-15)
To suppress optical aberrations caused by refractive index mismatch, we employ glycerol-immersion objectives in conjunction with fused silica cover glasses and imaging buffers with a high glycerol content. Here we demonstrate that the addition of glycerol to the buffer does
Ivan E Ivanov et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(11), 5853-5860 (2020-03-04)
The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of
Ki-Hee Song et al.
Light, science & applications, 9, 92-92 (2020-06-09)
Spectroscopic single-molecule localization microscopy (sSMLM) was used to achieve simultaneous imaging and spectral analysis of single molecules for the first time. Current sSMLM fundamentally suffers from a reduced photon budget because the photons from individual stochastic emissions are divided into
Wentao Shi et al.
Biosensors & bioelectronics, 51, 244-248 (2013-08-27)
This paper depicts a novel method to directionally stabilize enzymes together with redox mediators by electrodeposition. Chitosan was used as a stabilizing matrix. By electrochemical removal of local H(+), chitosan close to working electrode became locally insoluble, and enzymes and
Karnit Bahartan et al.
Chemical communications (Cambridge, England), 48(98), 11957-11959 (2012-11-06)
Yeast displaying glucose oxidase on their surface were encapsulated in a graphene oxide hydrogel. The ability of the modified yeast to reduce graphene oxide by glucose assimilation while maintaining viability was tested with time and deemed suitable for biofuel cell
Martin Lehmann et al.
Science advances, 5(11), eaax5775-eaax5775 (2019-12-07)
Clathrin-mediated endocytosis, an essential process for plasma membrane homeostasis and cell signaling, is characterized by stunning heterogeneity in the size and lifetime of clathrin-coated endocytic pits (CCPs). If and how CCP growth and lifetime are coupled and how this relates
Vahid Nasirimarekani et al.
Langmuir : the ACS journal of surfaces and colloids, 37(26), 7919-7927 (2021-06-17)
Suspensions of microtubules and nonadsorbing particles form thick and long bundles due to depletion forces. Such interactions act at the nanometer scale and define the structural and dynamical properties of the resulting networks. In this study, we analyze the depletion
Simone C Oostindie et al.
Nature biotechnology, 40(10), 1509-1519 (2022-07-26)
The use of therapeutic monoclonal antibodies is constrained because single antigen targets often do not provide sufficient selectivity to distinguish diseased from healthy tissues. We present HexElect®, an approach to enhance the functional selectivity of therapeutic antibodies by making their
Yibing Xie et al.
Materials science & engineering. C, Materials for biological applications, 33(8), 5028-5035 (2013-10-08)
The glucose oxidase (GOD) modified polypyrrole/titania nanotube enzyme electrode is fabricated for electrochemical biosensing application. The titania nanotube array is grown directly on a titanium substrate through an anodic oxidation process. A thin film of polypyrrole is coated onto titania
Tarlan Vatan et al.
STAR protocols, 2(4), 100971-100971 (2021-12-14)
Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach
Yanfang Lu et al.
Cell discovery, 7(1), 47-47 (2021-06-30)
The hematopoietic stem cell (HSC) niche has been extensively studied in bone marrow, yet a more systematic investigation into the microenvironment regulation of hematopoiesis in fetal liver is necessary. Here we investigate the spatial organization and transcriptional profile of individual
Jialei Tang et al.
Scientific reports, 7(1), 10945-10945 (2017-09-10)
We report a simple single-molecule fluorescence imaging method that increases the temporal resolution of any type of array detector by >5-fold with full field-of-view. We spread single-molecule spots to adjacent pixels by rotating a mirror in the detection path during
Alejo Mosqueira et al.
Journal of neurochemistry, 152(6), 663-674 (2019-11-30)
Synaptic strength depends on the number of cell-surface neurotransmitter receptors in dynamic equilibrium with intracellular pools. Dysregulation of this homeostatic balance occurs, for example in myasthenia gravis, an autoimmune disease characterized by a decrease in the number of postsynaptic nicotinic
Konstantin O Kutashev et al.
The Plant journal : for cell and molecular biology, 105(6), 1534-1548 (2020-12-15)
Arabidopsis thaliana 45S ribosomal genes (rDNA) are located in tandem arrays called nucleolus organizing regions on the termini of chromosomes 2 and 4 (NOR2 and NOR4) and encode rRNA, a crucial structural element of the ribosome. The current model of
Anna Lyubimova et al.
Nature protocols, 8(9), 1743-1758 (2013-08-21)
We present a protocol for visualizing and quantifying single mRNA molecules in mammalian (mouse and human) tissues. In the approach described here, sets of about 50 short oligonucleotides, each labeled with a single fluorophore, are hybridized to target mRNAs in
Lieke Koornneef et al.
PLoS genetics, 18(7), e1010046-e1010046 (2022-07-21)
Recombinases RAD51 and its meiosis-specific paralog DMC1 accumulate on single-stranded DNA (ssDNA) of programmed DNA double strand breaks (DSBs) in meiosis. Here we used three-color dSTORM microscopy, and a mouse model with severe defects in meiotic DSB formation and synapsis
Amit R Hood et al.
Materials science & engineering. C, Materials for biological applications, 33(7), 4313-4322 (2013-08-06)
This study describes the development of a novel bimetal (Fe and Cu)-grown hierarchical web of carbon micro-nanofiber-based electrode for biosensor applications, in particular to detect glucose in liquids. Carbon nanofibers (CNFs) are grown on activated carbon microfibers (ACFs) by chemical
Weng Man Chong et al.
eLife, 9 (2020-04-04)
Subdistal appendages (sDAPs) are centriolar elements that are observed proximal to the distal appendages (DAPs) in vertebrates. Despite the obvious presence of sDAPs, structural and functional understanding of them remains elusive. Here, by combining super-resolved localization analysis and CRISPR-Cas9 genetic
Erik Arango Gutierrez et al.
Biosensors & bioelectronics, 50, 84-90 (2013-07-10)
Glucose oxidase is an oxidoreductase exhibiting a high β-D-glucose specificity and high stability which renders glucose oxidase well-suited for applications in diabetes care. Nevertheless, GOx activity is highly oxygen dependent which can lead to inaccuracies in amperometric β-D-glucose determinations. Therefore
Lama Talje et al.
FEBS letters, 588(14), 2315-2320 (2014-05-27)
The kinesin-13 family of microtubule depolymerases is a major regulator of microtubule dynamics. RNA interference-induced knockdown studies have highlighted their importance in many cell division processes including spindle assembly and chromosome segregation. Since microtubule turnovers and most mitotic events are
Xianguo Jiang et al.
Biochemical pharmacology, 177, 114035-114035 (2020-05-16)
Noradrenaline (NA) promotes breakdown of the glucose-polymer, glycogen, and hence enhances glycolytic production of lactate in astrocytes. Here, in cultured rat cerebrocortical astrocytes, we examined the contributions of different adrenoceptor subtypes to NA-modulated glucose metabolism, and the relationship of NA-induced
Susanna Maria Früh et al.
Nature communications, 6, 7275-7275 (2015-06-05)
Fibronectin fibrils within the extracellular matrix play central roles in physiological and pathological processes, yet many structural details about their hierarchical and molecular assembly remain unknown. Here we combine site-specific protein labelling with single-molecule localization by stepwise photobleaching or direct
Diana C Odermatt et al.
PLoS genetics, 16(6), e1008740-e1008740 (2020-06-17)
FANCJ/BRIP1 is an iron-sulfur (FeS) cluster-binding DNA helicase involved in DNA inter-strand cross-link (ICL) repair and G-quadruplex (G4) metabolism. Mutations in FANCJ are associated with Fanconi anemia and an increased risk for developing breast and ovarian cancer. Several cancer-associated mutations
Chun Shi et al.
Biochimica et biophysica acta, 1830(3), 2538-2544 (2012-12-26)
Brain lipid peroxidation has long been considered a potential therapeutic target for Alzheimer's disease (AD). beta-sitosterol (BS), a plant sterol that is prevalent in plant plasma membrane, has been suggested to have antioxidant activity. Previous studies have demonstrated that dietary
Seokran Go et al.
Structure (London, England : 1993), 29(8), 810-822 (2021-06-19)
A steady supply of platelets maintains their levels in the blood, and this is achieved by the generation of progeny from platelet intermediates. Using systematic super-resolution microscopy, we examine the ultrastructural organization of various organelles in different platelet intermediates to
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