G7018

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Showing 1-30 of 31 results for "G7018" within Papers

Glucose-1-phosphate transport into protoplasts and chloroplasts from leaves of Arabidopsis.

Joerg Fettke et al.

Plant physiology, 155(4), 1723-1734 (2010-12-01)

Almost all glucosyl transfer reactions rely on glucose-1-phosphate (Glc-1-P) that either immediately acts as glucosyl donor or as substrate for the synthesis of the more widely used Glc dinucleotides, ADPglucose or UDPglucose. In this communication, we have analyzed two Glc-1-P-related

Substrate kinetics and substrate effects on the quaternary structure of barley UDP-glucose pyrophosphorylase.

Daniel Decker et al.

Phytochemistry, 79, 39-45 (2012-05-04)

UDP-Glc pyrophosphorylase (UGPase) is an essential enzyme responsible for production of UDP-Glc, which is used in hundreds of glycosylation reactions involving addition of Glc to a variety of compounds. In this study, barley UGPase was characterized with respect to effects

Enzymatic production of β-D-glucose-1-phosphate from trehalose.

Jef Van der Borght et al.

Biotechnology journal, 5(9), 986-993 (2010-08-28)

β-D-Glucose-1-phosphate (βGlc1P) is an efficient glucosyl donor for both enzymatic and chemical glycosylation reactions but is currently very costly and not available in large amounts. This article provides an efficient production method of βGlc1P from trehalose and phosphate using the

Identification of a novel UDP-sugar pyrophosphorylase with a broad substrate specificity in Trypanosoma cruzi.

Ting Yang et al.

The Biochemical journal, 429(3), 533-543 (2010-05-21)

The diverse types of glycoconjugates synthesized by trypanosomatid parasites are unique compared with the host cells. These glycans are required for the parasite survival, invasion or evasion of the host immune system. Synthesis of those glycoconjugates requires a constant supply

Earl Sutherland (1915-1974) [corrected] and the discovery of cyclic AMP.

Stanley A Blumenthal

Perspectives in biology and medicine, 55(2), 236-249 (2012-05-31)

In 1945, Earl Sutherland (1915-1974) [corrected] and associates began studies of the mechanism of hormone-induced glycogen breakdown in the liver. In 1956, their efforts culminated in the identification of cyclic AMP, an ancient molecule generated in many cell types in

The PGM3 gene encodes the major phosphoribomutase in the yeast Saccharomyces cerevisiae.

Thomas Walther et al.

FEBS letters, 586(23), 4114-4118 (2012-10-30)

The phosphoglucomutases (PGM) Pgm1, Pgm2, and Pgm3 of the yeast Saccharomyces cerevisiae were tested for their ability to interconvert ribose-1-phosphate and ribose-5-phosphate. The purified proteins were studied in vitro with regard to their kinetic properties on glucose-1-phosphate and ribose-1-phosphate. All

Phosphorolytic cleavage of sucrose by sucrose-grown ruminal bacterium Pseudobutyrivibrio ruminis strain k3.

K Stan-Glasek et al.

Folia microbiologica, 55(4), 383-385 (2010-08-04)

Rumen bacterium Pseudobutyrivibrio ruminis strain k3 utilized over 90% sucrose added to the growth medium as a sole carbon source. Zymographic studies of the bacterial cell extract revealed the presence of a single enzyme involved in sucrose digestion. Thin layer

The amino acid sequences in the C-terminal region of glucose-1-phosphate thymidylyltransferases determine their soluble expression in Escherichia coli.

Bijay Singh et al.

Protein engineering, design & selection : PEDS, 25(4), 179-187 (2012-02-16)

Two similar genes, dnmL and rmbA in Streptomyces peucetius, which encode for glucose-1-phosphate (G-1-P) thymidylyltransferases were expressed in Escherichia coli under similar conditions. While RmbA was expressed in soluble form, DnmL was found as insoluble aggregates in inclusion bodies. The

Structural basis for the broad substrate range of the UDP-sugar pyrophosphorylase from Leishmania major.

Achim Dickmanns et al.

Journal of molecular biology, 405(2), 461-478 (2010-11-16)

Nucleotide sugars and the enzymes that are responsible for their synthesis are indispensable for the production of complex carbohydrates and, thus, for elaboration of a protective cellular coat for many organisms such as the protozoan parasite Leishmania. These activated sugars

The binding of β-d-glucopyranosyl-thiosemicarbazone derivatives to glycogen phosphorylase: A new class of inhibitors.

Kyra-Melinda Alexacou et al.

Bioorganic & medicinal chemistry, 18(22), 7911-7922 (2010-10-16)

Glycogen phosphorylase (GP) is a promising target for the treatment of type 2 diabetes. In the process of structure based drug design for GP, a group of 15 aromatic aldehyde 4-(β-d-glucopyranosyl)thiosemicarbazones have been synthesized and evaluated as inhibitors of rabbit

Glucose-1-Phosphate-Induced Accumulation of Intracellular Starch: a Distinguishing Feature of Certain Corynebacteria.

Arden, S.

International J. Systemic Bact., 24, 139-141 (1974)

Resolving the sources of plasma glucose excursions following a glucose tolerance test in the rat with deuterated water and [U-13C]glucose.

Teresa C Delgado et al.

PloS one, 7(3), e34042-e34042 (2012-04-06)

Sources of plasma glucose excursions (PGE) following a glucose tolerance test enriched with [U-(13)C]glucose and deuterated water were directly resolved by (13)C and (2)H Nuclear Magnetic Resonance spectroscopy analysis of plasma glucose and water enrichments in rat. Plasma water (2)H-enrichment

Characterization of recombinant UDP- and ADP-glucose pyrophosphorylases and glycogen synthase to elucidate glucose-1-phosphate partitioning into oligo- and polysaccharides in Streptomyces coelicolor.

Matías D Asención Diez et al.

Journal of bacteriology, 194(6), 1485-1493 (2012-01-03)

Streptomyces coelicolor exhibits a major secondary metabolism, deriving important amounts of glucose to synthesize pigmented antibiotics. Understanding the pathways occurring in the bacterium with respect to synthesis of oligo- and polysaccharides is of relevance to determine a plausible scenario for

Engineering the allosteric properties of archaeal non-phosphorylating glyceraldehyde-3-phosphate dehydrogenases.

Fumiaki Ito et al.

Biochimica et biophysica acta, 1844(4), 759-766 (2014-02-05)

The archaeal non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN, EC 1.2.1.9) is a highly allosteric enzyme activated by glucose 1-phosphate (Glc1P). Recent kinetic analyses of two GAPN homologs from Sulfolobales show different allosteric behaviors toward the substrate glyceraldehyde-3-phosphate (GAP) and the allosteric effector

Adsorption, desorption, and surface-promoted hydrolysis of glucose-1-phosphate in aqueous goethite (α-FeOOH) suspensions.

Rickard Olsson et al.

Langmuir : the ACS journal of surfaces and colloids, 26(24), 18760-18770 (2010-11-20)

Adsorption, desorption, and precipitation reactions at environmental interfaces govern the fate of phosphorus in terrestrial and aquatic environments. Typically, a substantial part of the total pool of phosphorus consists of organophosphate, and in this study we have focused on the

Enzymatic hydrolysis of organic phosphates adsorbed on mineral surfaces.

Rickard Olsson et al.

Environmental science & technology, 46(1), 285-291 (2011-11-23)

Esters of phosphoric acid constitute a sizable fraction of the total phosphorus supply in the environment and thus play an important role in the global phosphorus cycle. Enzymatic hydrolysis of these esters to produce orthophosphate is often a required reaction

Structural and biochemical characterization of the N-terminal domain of flocculin Lg-Flo1p from Saccharomyces pastorianus reveals a unique specificity for phosphorylated mannose.

Lyann Sim et al.

The FEBS journal, 280(4), 1073-1083 (2013-01-04)

The mechanism of yeast flocculation is generally considered to be mediated through the interaction of cell surface flocculins and mannan carbohydrates. In the present study, the crystal structure of the soluble 25-kDa lectin domain of flocculin 1 from brewer's yeast

Phosphoglucomutase is absent in Trypanosoma brucei and redundantly substituted by phosphomannomutase and phospho-N-acetylglucosamine mutase.

Giulia Bandini et al.

Molecular microbiology, 85(3), 513-534 (2012-06-09)

The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyse the transfer of phosphate between the C6 and C1 hydroxyl groups of mannose, N-acetylglucosamine and glucose respectively. Although genes for a candidate PMM and a PAGM enzymes have

Multiple phenotypes in phosphoglucomutase 1 deficiency.

Laura C Tegtmeyer et al.

The New England journal of medicine, 370(6), 533-542 (2014-02-07)

Congenital disorders of glycosylation are genetic syndromes that result in impaired glycoprotein production. We evaluated patients who had a novel recessive disorder of glycosylation, with a range of clinical manifestations that included hepatopathy, bifid uvula, malignant hyperthermia, hypogonadotropic hypogonadism, growth

Characterization of the TDP-D-ravidosamine biosynthetic pathway: one-pot enzymatic synthesis of TDP-D-ravidosamine from thymidine-5-phosphate and glucose-1-phosphate.

Madan K Kharel et al.

Organic & biomolecular chemistry, 9(6), 1799-1808 (2011-01-26)

Ravidomycin V and related compounds, e.g., FE35A-B, exhibit potent anticancer activities against various cancer cell lines in the presence of visible light. The amino sugar moieties (D-ravidosamine and its analogues, respectively) in these molecules contribute to the higher potencies of

Characterization of hyperthermostable fructose-1,6-bisphosphatase from Thermococcus onnurineus NA1.

Yeol Gyun Lee et al.

Journal of microbiology (Seoul, Korea), 48(6), 803-807 (2011-01-12)

To understand the physiological functions of thermostable fructose-1,6-bisphosphatase (TNA1-Fbp) from Thermococcus onnurineus NA1, its recombinant enzyme was overexpressed in Escherichia coli, purified, and the enzymatic properties were characterized. The enzyme showed maximal activity for fructose-1,6-bisphosphate at 95°C and pH 8.0

Two carbon fluxes to reserve starch in potato (Solanum tuberosum L.) tuber cells are closely interconnected but differently modulated by temperature.

Joerg Fettke et al.

Journal of experimental botany, 63(8), 3011-3029 (2012-03-02)

Parenchyma cells from tubers of Solanum tuberosum L. convert several externally supplied sugars to starch but the rates vary largely. Conversion of glucose 1-phosphate to starch is exceptionally efficient. In this communication, tuber slices were incubated with either of four

Utilization of glycosyltransferases for the synthesis of a densely packed hyperbranched polysaccharide brush coating as artificial glycocalyx.

Jeroen van der Vlist et al.

Biomacromolecules, 12(10), 3728-3732 (2011-09-06)

Densely packed polysaccharide brushes consisting of α-D-glucose residues were grafted from modified silicon substrates. Potato phosphorylase was herein used to grow linear polysaccharide chains from silicon tethered maltoheptaose oligosaccharides using glucose-1-phosphate as donor substrate. The combined use of potato phosphorylase

Synthesis of branched polysaccharides with tunable degree of branching.

Jelena Ciric et al.

Carbohydrate polymers, 93(1), 31-37 (2013-03-08)

An in vitro enzyme-catalyzed tandem reaction using the enzymes phosphorylase b from rabbit muscle and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) to obtain branched polyglucans with tunable degree of branching (2% ÷ 13%) is presented. The tunable degree of

Transcriptome analysis suggests that starch synthesis may proceed via multiple metabolic routes in high yielding potato cultivars.

Kacper Piotr Kaminski et al.

PloS one, 7(12), e51248-e51248 (2013-01-04)

Glucose-6-phosphate is imported into the amyloplast of potato tubers and thought to constitute the precursor for starch synthesis in potato tubers. However, recently it was shown that glucose-1-phosphate can also be imported into the amyloplast and incorporated into starch via

Chitosan-based thermosensitive hydrogel as a promising ocular drug delivery system: preparation, characterization, and in vivo evaluation.

Xingwei Chen et al.

Journal of biomaterials applications, 27(4), 391-402 (2011-07-14)

The purpose of this study was to evaluate the feasibility of in situ thermosensitive hydrogel based on chitosan in combination with disodium α-d-Glucose 1-phosphate (DGP) for ocular drug delivery system. Aqueous solution of chitosan/DGP underwent sol-gel transition as temperature increased

A deeply divergent phosphoglucomutase (PGM) of Giardia lamblia has both PGM and phosphomannomutase activities.

Sanghamitra Mitra et al.

Glycobiology, 20(10), 1233-1240 (2010-05-29)

Giardia lamblia, which is an important parasitic cause of diarrhea, uses activated forms of glucose to make glycogen and activated forms of mannose to make glycophosphosphoinositol anchors. A necessary step for glucose activation is isomerization of glucose-6-phosphate to glucose-1-phosphate by

Inhibition of SnRK1 by metabolites: tissue-dependent effects and cooperative inhibition by glucose 1-phosphate in combination with trehalose 6-phosphate.

Cátia Nunes et al.

Plant physiology and biochemistry : PPB, 63, 89-98 (2012-12-22)

SnRK1 of the SNF1/AMPK group of protein kinases is an important regulatory protein kinase in plants. SnRK1 was recently shown as a target of the sugar signal, trehalose 6-phosphate (T6P). Glucose 6-phosphate (G6P) can also inhibit SnRK1 and given the

Allosteric regulation of the partitioning of glucose-1-phosphate between glycogen and trehalose biosynthesis in Mycobacterium tuberculosis.

Matías D Asención Diez et al.

Biochimica et biophysica acta, 1850(1), 13-21 (2014-10-04)

Mycobacterium tuberculosis is a pathogenic prokaryote adapted to survive in hostile environments. In this organism and other Gram-positive actinobacteria, the metabolic pathways of glycogen and trehalose are interconnected. In this work we show the production, purification and characterization of recombinant

Structural basis for the reaction mechanism of UDP-glucose pyrophosphorylase.

Hun Kim et al.

Molecules and cells, 29(4), 397-405 (2010-03-20)

UDP-glucose pyrophosphorylases (UGPase; EC 2.7.7.9) catalyze the conversion of UTP and glucose-1-phosphate to UDP-glucose and pyrophosphate and vice versa. Prokaryotic UGPases are distinct from their eukaryotic counterparts and are considered appropriate targets for the development of novel antibacterial agents since

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