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Showing 1-30 of 194 results for "h4272" within Papers
Junjun Xu et al.
Stem cell research & therapy, 10(1), 193-193 (2019-06-30)
Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models. In addition, transplantation studies using pig pluripotent stem (PS) cell derivatives may
Teresa Chioccarelli et al.
Cellular and molecular life sciences : CMLS, 79(1), 50-50 (2021-12-23)
Circular RNA (circRNA) biogenesis requires a backsplicing reaction, promoted by inverted repeats in cis-flanking sequences and trans factors, such as RNA-binding proteins (RBPs). Among these, FUS plays a key role. During spermatogenesis and sperm maturation along the epididymis such a
Nadine Kathagen et al.
Journal of cellular physiology, 228(10), 2071-2075 (2013-04-06)
The intracellular pH is regulated by a delicate balance of ion distribution across the plasma membrane and the physico-chemical properties of intra- and extracellular components. We analyzed the effects of glycosaminoglycans on the intracellular pH of fibroblasts by using the
Guang-Yu Bai et al.
Oncotarget, 8(41), 69863-69873 (2017-10-21)
Somatic cell nuclear transfer is an important technique for life science research, but its efficiency is still extremely low, and most genes that are important during early development, such as X chromosome-linked genes, are not appropriately expressed during this process.
Pedro N Moreira et al.
Methods in molecular biology (Clifton, N.J.), 349, 151-161 (2006-10-31)
Genomic-type transgenes are usually expressed in appropriate spatial- and temporal-specific manners. The largest genomic transgenes can be prepared using yeast artificial chromosomes (YACs). Normally, YAC transgenic mice are produced by standard pro-nuclear microinjection, although other methods, involving the use of
Lisa A Gurski et al.
PloS one, 7(11), e50075-e50075 (2012-11-21)
To study the individual functions of hyaluronan interacting proteins in prostate cancer (PCa) motility through connective tissues, we developed a novel three-dimensional (3D) hyaluronic acid (HA) hydrogel assay that provides a flexible, quantifiable, and physiologically relevant alternative to current methods.
Marcos Plana-Carmona et al.
Stem cell reports, 17(9), 1991-2004 (2022-08-13)
IL-6 has been shown to be required for somatic cell reprogramming into induced pluripotent stem cells (iPSCs). However, how Il6 expression is regulated and whether it plays a role during embryo development remains unknown. Here, we describe that IL-6 is
Andres F Sanchez-Teran et al.
Veterinary surgery : VS, 45(2), 223-230 (2016-01-16)
To evaluate the effect of arthroscopic lavage on systemic serum amyloid A (SAA) and SAA, total protein, nucleated cell count, and percentage of neutrophils in synovial fluid in healthy horses. Prospective experimental study. Healthy adult horses (n = 6). Middle carpal joints
M Rajan et al.
International journal of pharmaceutics, 453(2), 514-522 (2013-06-26)
This study examines the performance of novel hyaluronidase enzyme core-5-fluorouracil-loaded chitosan-polyethylene glycol-gelatin polymer nanocomposites, which were prepared using an ionic gelation technique, as targeted and controlled drug delivery vehicles. These hyaluronidase-loaded nanoparticles have recently been proposed as targeted and controlled
Yin-Li Zhang et al.
EMBO molecular medicine, 13(12), e14887-e14887 (2021-11-16)
Early embryonic arrest and fragmentation (EEAF) is a common phenomenon leading to female infertility, but the genetic determinants remain largely unknown. The Moloney sarcoma oncogene (MOS) encodes a serine/threonine kinase that activates the ERK signaling cascade during oocyte maturation in
Z-F Lin et al.
European review for medical and pharmacological sciences, 16(9), 1249-1256 (2012-10-11)
Lung adenocarcinoma (LAC) is the most common type of lung cancer, accounting for 30-35% of all cases. In this study we aim to predict potential genes and confirm pathways which are associated with LAC. By using the meta-analysis method, GSE10072
Olga N Uchakina et al.
Clinical immunology (Orlando, Fla.), 146(1), 56-69 (2012-12-19)
We investigated the role of the extracellular matrix component, hyaluronic acid (HA) in SEB-induced ALI/ARDS. Intranasal exposure of mice to SEB led to a significant increase in the level of soluble hyaluronic acid in the lungs. Similarly, in an endothelial
Neelesh Kumar Nema et al.
Pharmaceutical biology, 51(9), 1182-1187 (2013-06-15)
Centella asiatica (L.) Urban (Apiaceae), a valuable herb described in Ayurveda, is used in the indigenous system of medicine as a tonic to treat skin diseases. Centella asiatica methanol extract and its ethyl acetate, n-butanol and aqueous fraction, were subjected
Alex J Harper
Methods in molecular medicine, 104, 185-202 (2004-09-30)
Manipulation of the rodent genome by deliberately inserting (transgenic) or removing (knockout) a gene of interest or indeed by selectively breeding animals with a spontaneous or random mutation producing a trait of interest has been developed over several years. Mouse
Brandon Buckway et al.
International journal of pharmaceutics, 456(1), 202-211 (2013-08-13)
Delivery of macromolecules to pancreatic cancer is inhibited by a dense extracellular matrix composed of hyaluronic acid, smooth muscle actin and collagen fibers. Hyaluronic acid causes a high intratumoral fluidic pressure which prevents diffusion and penetration into the pancreatic tumor.
Yan Jiang et al.
Journal of assisted reproduction and genetics, 30(9), 1175-1179 (2013-08-31)
To study the efficacy of percutaneous epididymal sperm aspiration (PESA) in combination with short time insemination to treat infertile men with obstructive azoospermia (OA). Paired randomized controlled trial in which each couple's cohort of oocytes was divided into two equal
Yuan Gao et al.
STAR protocols, 4(2), 102247-102247 (2023-04-22)
Cell-free DNA (cfDNA) in spent embryo culture media (SECM) provides prospects for noninvasive preimplantation genetic testing. Here, we present a post-bisulfite-adapter-tagging (PBAT)-based whole-genome DNA methylation sequencing protocol (SECM-PBAT) for human SECM cfDNA analysis. We describe steps for SECM lysis, bisulfite
Maya Imbrechts et al.
iScience, 25(8), 104705-104705 (2022-07-12)
Treatment with neutralizing monoclonal antibodies (mAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contributes to COVID-19 management. Unfortunately, SARS-CoV-2 variants escape several of these recently approved mAbs, highlighting the need for additional discovery and development. In a convalescent patient
Ionara Diniz E S Barcelos et al.
Reproductive biomedicine online, 30(5), 532-541 (2015-03-17)
Aromatase plays a fundamental role in the establishment of oocyte quality, which might be compromised in infertile women with endometriosis. The expression of the CYP19A1 gene (that encodes aromatase) was compared in cumulus cells and oestradiol concentrations in the follicular
Xiaoming Liu et al.
Small (Weinheim an der Bergstrasse, Germany), 15(5), e1804421-e1804421 (2018-12-18)
A noncontact method that can achieve immobilization, transportation, and rotation in the microscale is desired in biological micromanipulation. A multifunctional noncontact micromanipulation method is proposed here based on a vibration-generated whirling flow. Resonance of a cantilever structure is utilized to
CRISPR/Cas9 Endonuclease-Mediated Mouse Genome Editing of One-Cell and/or Two-Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Interhomolog Repair Mechanism.
Garza, et al.
Methods in Molecular Biology, 2631, 253-266 (2023)
Tegginamath Gireesh et al.
Archiv der Pharmazie, 346(9), 645-653 (2013-08-03)
A novel series of Biginelli 2-3 (a and b) and Biginelli-like compounds 4-7 (a and b) were synthesized from 3-aryl-4-formylsydnone 1 (a and b). Since the crystal structure of hyaluronidase was unavailable, the human hyaluronidase protein structure was used as
Erdenechimeg Selenge et al.
Journal of natural medicines, 67(4), 867-875 (2013-02-12)
Activity-guided isolation of the n-butanol fraction of Chamaerhodos erecta and water soluble fraction of C. altaica resulted in the isolation of 39 compounds, including new compounds identified as 4,5-dihydroxybenzaldehyde-3-O-β-D-glucopyranoside (1) from C. erecta and quercetin-3-O-β-D-glucuronopyranosyl-4'-O-β-D-glucopyranoside (2) from C. altaica. A
Casper B Foldager et al.
Acta orthopaedica, 82(2), 234-240 (2011-03-26)
In vitro expansion of autologous chondrocytes is an essential part of many clinically used cartilage repair treatments. Native chondrocytes reside in a 3-dimensional (3D) network and are exposed to low levels of oxygen. We compared monolayer culture to combined 3D
Mehul S Patel et al.
Journal of cachexia, sarcopenia and muscle, 7(4), 436-448 (2016-05-31)
Loss of muscle mass is a co-morbidity common to a range of chronic diseases including chronic obstructive pulmonary disease (COPD). Several systemic features of COPD including increased inflammatory signalling, oxidative stress, and hypoxia are known to increase the expression of
Rapid and efficient degradation of endogenous proteins inA vivo identifies stage-specific roles of RNA Pol II pausing in mammalian development.
Abuhashem, et al.
Developmental Cell, 57, 1068-1080 (2023)
Fei Gao et al.
The Journal of biological chemistry, 283(10), 6058-6066 (2008-01-01)
Extracellular superoxide dismutase (EC-SOD) is expressed at high levels in lungs. EC-SOD has a polycationic matrix-binding domain that binds to polyanionic constituents in the matrix. Previous studies indicate that EC-SOD protects the lung in both bleomycin- and asbestos-induced models of
Carl Marfurt et al.
Investigative ophthalmology & visual science, 60(15), 4972-4984 (2019-12-04)
The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe
STAT3 Is an Upstream Regulator of Granzyme G in the Maternal-To-Zygotic Transition of Mouse Embryos.
Huan Ou-Yang et al.
International journal of molecular sciences, 22(1) (2021-01-21)
The maternal-to-zygotic transition (MZT), which controls maternal signaling to synthesize zygotic gene products, promotes the preimplantation development of mouse zygotes to the two-cell stage. Our previous study reported that mouse granzyme g (Gzmg), a serine-type protease, is required for the
Pedro N Moreira et al.
Methods in molecular biology (Clifton, N.J.), 1194, 141-156 (2014-07-30)
Over the years many well-described techniques for the introduction of transgene DNA into host organisms have been used, including pronuclear injection, in vitro fertilization-mediated transgenesis, transfection of ES and spermatogenic cells, nuclear transfer of somatic cell nuclei, and lentiviral vectors.
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