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Showing 31-60 of 194 results for "H4272" within Papers
Xiaoming Liu et al.
Small (Weinheim an der Bergstrasse, Germany), 15(5), e1804421-e1804421 (2018-12-18)
A noncontact method that can achieve immobilization, transportation, and rotation in the microscale is desired in biological micromanipulation. A multifunctional noncontact micromanipulation method is proposed here based on a vibration-generated whirling flow. Resonance of a cantilever structure is utilized to
Marcos Plana-Carmona et al.
Stem cell reports, 17(9), 1991-2004 (2022-08-13)
IL-6 has been shown to be required for somatic cell reprogramming into induced pluripotent stem cells (iPSCs). However, how Il6 expression is regulated and whether it plays a role during embryo development remains unknown. Here, we describe that IL-6 is
Olga N Uchakina et al.
Clinical immunology (Orlando, Fla.), 146(1), 56-69 (2012-12-19)
We investigated the role of the extracellular matrix component, hyaluronic acid (HA) in SEB-induced ALI/ARDS. Intranasal exposure of mice to SEB led to a significant increase in the level of soluble hyaluronic acid in the lungs. Similarly, in an endothelial
Luping Yu et al.
Development (Cambridge, England), 148(2) (2020-12-16)
Alternative splicing (AS) contributes to gene diversification, but the AS program during germline development remains largely undefined. Here, we interrupted pre-mRNA splicing events controlled by epithelial splicing regulatory protein 1 (ESRP1) and found that it induced female infertility in mice.
Robert B Vernon et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 69(6), 415-428 (2021-06-04)
Although many studies have focused on a role for hyaluronan (HA) of interstitial extracellular matrix (presumably produced by non-vascular "stromal" cells) in regulating vascular growth, we herein examine the influence of "autocrine HA" produced by vascular endothelial cells themselves on
David J Barry et al.
Journal of cell science, 135(10) (2022-05-04)
The study of cellular and developmental processes in physiologically relevant three-dimensional (3D) systems facilitates an understanding of mechanisms underlying cell fate, disease and injury. While cutting-edge microscopy technologies permit the routine acquisition of 3D datasets, there is currently a limited
Mehul S Patel et al.
Journal of cachexia, sarcopenia and muscle, 7(4), 436-448 (2016-05-31)
Loss of muscle mass is a co-morbidity common to a range of chronic diseases including chronic obstructive pulmonary disease (COPD). Several systemic features of COPD including increased inflammatory signalling, oxidative stress, and hypoxia are known to increase the expression of
Christina Tenenhaus Dann et al.
Methods in molecular biology (Clifton, N.J.), 450, 193-209 (2008-03-29)
The primary method for determining the function of a gene in rodents has been to make a knockout mouse through homologous recombination in embryonic stem cells. However, with the advent of RNA interference (RNAi) technology, new methods for studying gene
Vadim Cherezov et al.
Structure (London, England : 1993), 16(9), 1378-1388 (2008-09-13)
The crystal structures of the cytoplasmic domain of the putative zinc transporter CzrB in the apo and zinc-bound forms reported herein are consistent with the protein functioning in vivo as a homodimer. NMR, X-ray scattering, and size-exclusion chromatography provide support
Casper B Foldager et al.
Acta orthopaedica, 82(2), 234-240 (2011-03-26)
In vitro expansion of autologous chondrocytes is an essential part of many clinically used cartilage repair treatments. Native chondrocytes reside in a 3-dimensional (3D) network and are exposed to low levels of oxygen. We compared monolayer culture to combined 3D
Sanghyun Lee et al.
Autophagy, 18(5), 1062-1077 (2021-09-15)
Mutations in the macroautophagy/autophagy gene EPG5 are responsible for Vici syndrome, a human genetic disease characterized by combined immunodeficiency. Previously, we found that epg5-/- mice exhibit hyperinflammation in the lungs mediated by IL1B/IL-1β and TNF/TNFα, resulting in resistance to influenza.
Fernando H Biase
STAR protocols, 2(4), 100895-100895 (2021-11-02)
Studying individual mammalian oocytes has been extremely valuable for the understanding of the molecular composition of oocytes including RNA storage. Here, a detailed protocol for isolation of oocytes, extraction of total RNA from single oocytes followed by full-length cDNA amplification
Rapid and efficient degradation of endogenous proteins inA vivo identifies stage-specific roles of RNA Pol II pausing in mammalian development.
Abuhashem, et al.
Developmental Cell, 57, 1068-1080 (2023)
Tegginamath Gireesh et al.
Archiv der Pharmazie, 346(9), 645-653 (2013-08-03)
A novel series of Biginelli 2-3 (a and b) and Biginelli-like compounds 4-7 (a and b) were synthesized from 3-aryl-4-formylsydnone 1 (a and b). Since the crystal structure of hyaluronidase was unavailable, the human hyaluronidase protein structure was used as
Fang Gao et al.
Stem cell reviews, 13(4), 532-541 (2017-01-08)
Factor-based induced reprogramming approaches have tremendous potential for human regenerative medicine, but the efficiencies of these approaches are still low. In this study, we analyzed the global transcriptional profiles of mouse induced pluripotent stem cells (miPSCs) and mouse embryonic stem
Xue Jiang et al.
Nature communications, 14(1), 3729-3729 (2023-06-23)
In mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post-transcriptional levels. However, the factors underlying this sophisticated but explicit process remain largely unclear. Here we characterize the function
Kun Huang et al.
Frontiers in molecular neuroscience, 13, 154-154 (2020-10-30)
The neuromuscular junction (NMJ) is a prototypic chemical synapse between the spinal motor neuron and the motor endplate. Gene expression profiles of the motor endplate are not fully elucidated. Collagen Q (ColQ) is a collagenic tail subunit of asymmetric forms
Mattia Quattrocelli et al.
PLoS genetics, 13(10), e1007070-e1007070 (2017-10-25)
Genetic disruption of the dystrophin complex produces muscular dystrophy characterized by a fragile muscle plasma membrane leading to excessive muscle degeneration. Two genetic modifiers of Duchenne Muscular Dystrophy implicate the transforming growth factor β (TGFβ) pathway, osteopontin encoded by the
Junjun Xu et al.
Stem cell research & therapy, 10(1), 193-193 (2019-06-30)
Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models. In addition, transplantation studies using pig pluripotent stem (PS) cell derivatives may
Robert Kumagai et al.
Free radical biology & medicine, 47(5), 600-607 (2009-06-09)
Although the glycocalyx has been implicated in wall shear stress (WSS) mechanotransduction, the role of glycocalyx components in nitric oxide (NO) and reactive oxygen species (ROS) production remains unclear. Here, we tested the hypothesis that glycocalyx is implicated in both
Yifei Dong et al.
Scientific reports, 6, 36928-36928 (2016-11-22)
The extracellular matrix glycosaminoglycan, hyaluronan, has been described as a regulator of tissue inflammation, with hyaluronan fragments reported to stimulate innate immune cells. High molecular mass hyaluronan is normally present in tissues, but upon inflammation lower molecular mass fragments are
Pedro N Moreira et al.
Methods in molecular biology (Clifton, N.J.), 1194, 141-156 (2014-07-30)
Over the years many well-described techniques for the introduction of transgene DNA into host organisms have been used, including pronuclear injection, in vitro fertilization-mediated transgenesis, transfection of ES and spermatogenic cells, nuclear transfer of somatic cell nuclei, and lentiviral vectors.
Bingbo Shi et al.
Stem cell research & therapy, 11(1), 505-505 (2020-11-29)
Despite years of research, porcine-induced pluripotent stem cells (piPSCs) with germline chimeric capacity have not been established. Furthermore, the key transcription factors (TFs) defining the naïve state in piPSCs also remain elusive, even though TFs in the inner cell mass
Neelesh Kumar Nema et al.
Pharmaceutical biology, 51(9), 1182-1187 (2013-06-15)
Centella asiatica (L.) Urban (Apiaceae), a valuable herb described in Ayurveda, is used in the indigenous system of medicine as a tonic to treat skin diseases. Centella asiatica methanol extract and its ethyl acetate, n-butanol and aqueous fraction, were subjected
Da-Eun Um et al.
Reproductive biology and endocrinology : RB&E, 18(1), 37-37 (2020-05-06)
We had previously demonstrated that vitrification reduces the levels of certain phospholipid classes, and that oocytes from aged mice show a similar lipidome alteration, even without vitrification. In the current investigation, we examined if vitrification-warming of mouse oocytes from young
Timothy Corcoran Flynn et al.
Plastic and reconstructive surgery, 132(4 Suppl 2), 22S-32S (2013-10-01)
In this study, the authors sought to determine the molecular weight distribution of three hyaluronic acids-Belotero Balance, Restylane, and Juvéderm Ultra-and their rates of degradation following exposure to hyaluronidase. Lot consistency of Belotero Balance also was analyzed. Three lots of
Pu Liu et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 33(1), 53-59 (2009-12-03)
Cyclophosphamide (CTX) is often used to create a "window" for more effective therapeutic tumor vaccination. According to a commonly applied protocol, we injected 2 mg CTX intraperitoneally to mice with small (2 to 3 mm diameter) or large (5 to
Marnie Preston et al.
Annals of neurology, 73(2), 266-280 (2013-03-07)
Oligodendrocyte progenitor cells (OPCs) recruited to demyelinating lesions often fail to mature into oligodendrocytes (OLs) that remyelinate spared axons. The glycosaminoglycan hyaluronan (HA) accumulates in demyelinating lesions and has been implicated in the failure of OPC maturation and remyelination. We
Jing-hong Chen et al.
Journal of Zhejiang University. Science. B, 9(6), 455-463 (2008-06-11)
To investigate the effects of T-2 toxin on expressions of Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 on human chondrocytes. Human chondrocytes were treated with T-2 toxin (1-20 ng/ml) for 5 d. Fas, p53 and other apoptosis-related proteins such as
Andriy Yabluchanskiy et al.
GeroScience, 42(2), 409-428 (2020-01-22)
Whole brain irradiation (WBI, also known as whole brain radiation therapy or WBRT) is a mainstream therapy for patients with identifiable brain metastases and as a prophylaxis for microscopic malignancies. WBI accelerates brain aging, causing progressive cognitive dysfunction in ~ 50%
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