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Showing 31-60 of 194 results for "H4272" within Papers
Fernando H Biase
STAR protocols, 2(4), 100895-100895 (2021-11-02)
Studying individual mammalian oocytes has been extremely valuable for the understanding of the molecular composition of oocytes including RNA storage. Here, a detailed protocol for isolation of oocytes, extraction of total RNA from single oocytes followed by full-length cDNA amplification
Sanghyun Lee et al.
Autophagy, 18(5), 1062-1077 (2021-09-15)
Mutations in the macroautophagy/autophagy gene EPG5 are responsible for Vici syndrome, a human genetic disease characterized by combined immunodeficiency. Previously, we found that epg5-/- mice exhibit hyperinflammation in the lungs mediated by IL1B/IL-1β and TNF/TNFα, resulting in resistance to influenza.
Marina Gertsenstein et al.
Current protocols in mouse biology, 8(1), 28-53 (2018-07-25)
Mice carrying patient-associated point mutations are powerful tools to define the causality of single-nucleotide variants to disease states. Epitope tags enable immuno-based studies of genes for which no antibodies are available. These alleles enable detailed and precise developmental, mechanistic, and
Steven J Foltz et al.
The Journal of cell biology, 220(3) (2021-01-27)
Mutations in ANO5 (TMEM16E) cause limb-girdle muscular dystrophy R12. Defective plasma membrane repair is a likely mechanism. Using myofibers from Ano5 knockout mice, we show that trafficking of several annexin proteins, which together form a cap at the site of
Da-Eun Um et al.
Reproductive biology and endocrinology : RB&E, 18(1), 37-37 (2020-05-06)
We had previously demonstrated that vitrification reduces the levels of certain phospholipid classes, and that oocytes from aged mice show a similar lipidome alteration, even without vitrification. In the current investigation, we examined if vitrification-warming of mouse oocytes from young
Timothy Corcoran Flynn et al.
Plastic and reconstructive surgery, 132(4 Suppl 2), 22S-32S (2013-10-01)
In this study, the authors sought to determine the molecular weight distribution of three hyaluronic acids-Belotero Balance, Restylane, and Juvéderm Ultra-and their rates of degradation following exposure to hyaluronidase. Lot consistency of Belotero Balance also was analyzed. Three lots of
Bingbo Shi et al.
Stem cell research & therapy, 11(1), 505-505 (2020-11-29)
Despite years of research, porcine-induced pluripotent stem cells (piPSCs) with germline chimeric capacity have not been established. Furthermore, the key transcription factors (TFs) defining the naïve state in piPSCs also remain elusive, even though TFs in the inner cell mass
Débora Laís Justo Jacomini et al.
Toxicon : official journal of the International Society on Toxinology, 64, 70-80 (2013-01-12)
In this study, we describe the cDNA cloning, sequencing, and 3-D structure of the allergen hyaluronidase from Polybia paulista venom (Pp-Hyal). Using a proteomic approach, the native form of Pp-Hyal was purified to homogeneity and used to produce a Pp-specific
Chiara M Cattaneo et al.
Nature protocols, 15(1), 15-39 (2019-12-20)
T cells are key players in cancer immunotherapy, but strategies to expand tumor-reactive cells and study their interactions with tumor cells at the level of an individual patient are limited. Here we describe the generation and functional assessment of tumor-reactive
Fabrizzio Horta et al.
Journal of assisted reproduction and genetics, 38(1), 55-69 (2020-10-18)
Oocyte quality and reproductive outcome are negatively affected by advanced maternal age, ovarian stimulation and method of oocyte maturation during assisted reproduction; however, the mechanisms responsible for these associations are not fully understood. The aim of this study was to
Robert Kumagai et al.
Free radical biology & medicine, 47(5), 600-607 (2009-06-09)
Although the glycocalyx has been implicated in wall shear stress (WSS) mechanotransduction, the role of glycocalyx components in nitric oxide (NO) and reactive oxygen species (ROS) production remains unclear. Here, we tested the hypothesis that glycocalyx is implicated in both
Luping Yu et al.
Development (Cambridge, England), 148(2) (2020-12-16)
Alternative splicing (AS) contributes to gene diversification, but the AS program during germline development remains largely undefined. Here, we interrupted pre-mRNA splicing events controlled by epithelial splicing regulatory protein 1 (ESRP1) and found that it induced female infertility in mice.
Robert B Vernon et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 69(6), 415-428 (2021-06-04)
Although many studies have focused on a role for hyaluronan (HA) of interstitial extracellular matrix (presumably produced by non-vascular "stromal" cells) in regulating vascular growth, we herein examine the influence of "autocrine HA" produced by vascular endothelial cells themselves on
David J Barry et al.
Journal of cell science, 135(10) (2022-05-04)
The study of cellular and developmental processes in physiologically relevant three-dimensional (3D) systems facilitates an understanding of mechanisms underlying cell fate, disease and injury. While cutting-edge microscopy technologies permit the routine acquisition of 3D datasets, there is currently a limited
Yifei Dong et al.
Scientific reports, 6, 36928-36928 (2016-11-22)
The extracellular matrix glycosaminoglycan, hyaluronan, has been described as a regulator of tissue inflammation, with hyaluronan fragments reported to stimulate innate immune cells. High molecular mass hyaluronan is normally present in tissues, but upon inflammation lower molecular mass fragments are
Christina Tenenhaus Dann et al.
Methods in molecular biology (Clifton, N.J.), 450, 193-209 (2008-03-29)
The primary method for determining the function of a gene in rodents has been to make a knockout mouse through homologous recombination in embryonic stem cells. However, with the advent of RNA interference (RNAi) technology, new methods for studying gene
Vadim Cherezov et al.
Structure (London, England : 1993), 16(9), 1378-1388 (2008-09-13)
The crystal structures of the cytoplasmic domain of the putative zinc transporter CzrB in the apo and zinc-bound forms reported herein are consistent with the protein functioning in vivo as a homodimer. NMR, X-ray scattering, and size-exclusion chromatography provide support
Guang-Yu Bai et al.
Zygote (Cambridge, England), 26(1), 24-32 (2017-12-15)
Somatic cell nuclear transfer (SCNT) is an important technique for life science research. However, most SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we show that abnormal Xi occurs in somatic cell NT blastocysts, whereas
P Lorenzo Bozzelli et al.
Experimental neurology, 323, 113077-113077 (2019-11-05)
HIV-associated neurocognitive disorders (HAND) continue to persist despite effective control of viral replication. Although the mechanisms underlying HAND are poorly understood, recent attention has focused on altered neuronal population activity as a correlate of impaired cognition. However, while alterations in
J Maselli et al.
Andrology, 2(2), 259-266 (2014-01-31)
Treatment of testicular cancer includes the coadministration of bleomycin, etoposide and cis-platinum (BEP); however, along with its therapeutic benefit, BEP exposure results in extensive reproductive chemotoxic effects, including alterations to sperm chromatin integrity. As an intact paternal genome is essential
The transglycosylative action of testicular hyaluronidase.
B WEISSMANN
The Journal of biological chemistry, 216(2), 783-794 (1955-10-01)
Satoshi Kishigami et al.
Methods in molecular biology (Clifton, N.J.), 1048, 109-126 (2013-08-10)
In mammals, a diploid genome following fertilization of haploid cells, an egg, and a spermatozoon is unique and irreproducible. This implies that the generated unique diploid genome is doomed with the individual's inevitable demise. Since it was first reported in
Michele Gomes Da Broi et al.
Antioxidants (Basel, Switzerland), 11(6) (2022-06-25)
Endometriosis-related infertility is associated with oxidative stress (OS). The present study aims to compare serum OS markers of infertile women with endometriosis and controls during the follicular phase of the natural cycle (D1), after pituitary downregulation using a GnRH agonist
Vera Volfova et al.
PLoS neglected tropical diseases, 2(9), e294-e294 (2008-09-30)
Salivary hyaluronidases have been described in a few bloodsucking arthropods. However, very little is known about the presence of this enzyme in various bloodsucking insects and no data are available on its effect on transmitted microorganisms. Here, we studied hyaluronidase
Mattia Quattrocelli et al.
PLoS genetics, 13(10), e1007070-e1007070 (2017-10-25)
Genetic disruption of the dystrophin complex produces muscular dystrophy characterized by a fragile muscle plasma membrane leading to excessive muscle degeneration. Two genetic modifiers of Duchenne Muscular Dystrophy implicate the transforming growth factor β (TGFβ) pathway, osteopontin encoded by the
Yan Geng et al.
Nature communications, 13(1), 7114-7114 (2022-11-20)
Pulmonary fibrosis is a chronic interstitial lung disease that causes irreversible and progressive lung scarring and respiratory failure. Activation of fibroblasts plays a central role in the progression of pulmonary fibrosis. Here we show that platelet endothelial aggregation receptor 1
Kate A Redgrove et al.
PloS one, 7(11), e50851-e50851 (2012-12-05)
A common defect encountered in the spermatozoa of male infertility patients is an idiopathic failure of sperm-egg recognition. In order to resolve the molecular basis of this condition we have compared the proteomic profiles of spermatozoa exhibiting an impaired capacity
Benjamin J Patty et al.
Nature protocols, 16(5), 2633-2666 (2021-04-30)
Determining chromatin-associated protein localization across the genome has provided insight into the functions of DNA-binding proteins and their connections to disease. However, established protocols requiring large quantities of cell or tissue samples currently limit applications for clinical and biomedical research
Séverine Remy et al.
Methods in molecular biology (Clifton, N.J.), 597, 109-125 (2009-12-17)
Lentiviral vectors are now well recognized as good vehicles for gene delivery. This is because they can efficiently transduce both dividing and post-mitotic cells, and stably integrate into the host genome allowing for long-term expression of the transgene. Their potential
Luciano Alves Favorito et al.
International braz j urol : official journal of the Brazilian Society of Urology, 38(6), 802-808 (2013-01-11)
To evaluate histological alterations in prepuce of patients with phimosis submitted to topic treatment with betamethasone in association with hyaluronidase. We studied sixty patients (mean age 4.5), presenting true phimosis and treated with a topical treatment with betamethasone cream (0.2%)
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