lichropur® high purity acids for hplc and lc-ms analysis

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Showing 1-30 of 96 results for "lichropur® high purity acids for hplc and lc-ms analysis" within Papers

Injectable peptide hydrogel as intraperitoneal triptolide depot for the treatment of orthotopic hepatocellular carcinoma.

Xiyue Zhao et al.

Acta pharmaceutica Sinica. B, 9(5), 1050-1060 (2019-10-28)

Chemotherapy is among the limited choices approved for the treatment of hepatocellular carcinoma (HCC) at intermediate and advanced stages. Preferential and prolonged drug exposure in diseased sites is required to maximize the therapeutic index of the drug. Here, we report

Isolation and identification of myo-inositol crystals from dragon fruit (Hylocereus polyrhizus).

Ow Phui San Rebecca et al.

Molecules (Basel, Switzerland), 17(4), 4583-4594 (2012-04-19)

Crystals isolated from Hylocereus polyrhizus were analyzed using four different approaches--X-ray Crystallography, High Performance Liquid Chromatography (HPLC), Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) and Nuclear Magnetic Resonance (NMR) and identified as myo-inositol. The X-ray crystallography analysis showed that the unit-cell

Xanthohumol C, a minor bioactive hop compound: Production, purification strategies and antimicrobial test.

Simon Roehrer et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1095, 39-49 (2018-07-28)

Hop has been attracting scientific attention due to its favorable bioactivity properties. It is thus desirable to relate these properties to the specific hop compounds and extract these compounds in highly purified form in order to enhance the effect. The

Purification of chlorogenic acid from Heijingang potatoes and evaluation of its binding properties to recombinant human serum albumin.

Xueli Zhang et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1110-1111, 87-93 (2019-02-21)

The aim of the present study was to purify the natural chlorogenic acid (CGA) monomer from Heijingang potatoes and investigate its interaction with recombinant human serum albumin (rHSA). The potato extract (PE) was purified using macroporous resins and solvent, and

Rapid and efficient purification of chrysophanol in Rheum Palmatum LINN by supercritical fluid extraction coupled with preparative liquid chromatography in tandem.

Tiffany Chien-Ting Lo et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 893-894, 101-106 (2012-03-23)

Chrysophanol has high pharmaceutical values. However, it was difficult to use the traditional extraction method to extract high-concentration chrysophanol. Therefore, the purpose of this study is to purify and separate chrysophanol in traditional herb, Rheum Palmatum LINN, by using supercritical

An efficient HPLC method for the analysis of isomeric purity of technetium-99m-exametazime and identity confirmation using LC-MS.

D Vanderghinste et al.

Journal of pharmaceutical and biomedical analysis, 32(4-5), 679-685 (2003-08-06)

99mTc-exametazime (99mTc-d,l-HMPAO, 99mTc-d,l-hexamethylpropyleneamine oxime) is a neutral rather unstable complex of short-lived 99mTc (t(1/2)=6 h) with the d,l-isomer (mixture of D,D- and L,L-isomers) of a bis-amine bis-oxime tetraligand. It is widely used for measurement of regional cerebral perfusion in nuclear

Isolation and structure elucidation of major alkaline degradant of ezetimibe.

Anuradha K Gajjar et al.

Journal of pharmaceutical and biomedical analysis, 55(1), 225-229 (2011-02-04)

This work presents the isolation and characterization of the alkaline degradant of Ezetimibe. Ezetimibe, a selective inhibitor of intestinal cholesterol absorption, was subjected to alkaline degradation. Ezetimibe was reacted with 0.1M methanolic sodium hydroxide solution for 10min at 80°C to

Chemical synthesis and NMR characterization of structured polyunsaturated triacylglycerols.

Laëtitia Fauconnot et al.

Chemistry and physics of lipids, 139(2), 125-136 (2006-01-13)

The chemical synthesis of pure triacylglycerol (TAG) regioisomers, that contain long chain polyunsaturated fatty acids, such as arachidonic acid (AA) or docosahexaenoic acid (DHA), and saturated fatty acids, such as lauric acid (La) or palmitic acid (P), at defined positions

Quality control of cationic cell-penetrating peptides.

Sofie Stalmans et al.

Journal of pharmaceutical and biomedical analysis, 117, 289-297 (2015-09-24)

During fundamental research, it is recommended to evaluate the test compound identity and purity in order to obtain reliable study outcomes. For peptides, quality control (QC) analyses are routinely performed using reversed-phase liquid chromatography coupled to an ultraviolet (UV) detector

Rapid screening and detection of XOD inhibitors from S. tamariscina by ultrafiltration LC-PDA-ESI-MS combined with HPCCC.

Jing Wang et al.

Analytical and bioanalytical chemistry, 406(28), 7379-7387 (2014-09-23)

Xanthine oxidase (XOD) catalyzes the metabolism of hypoxanthine and xanthine to uric acid, the overproduction of which could cause hyperuricemia, a risk factor for gout. Inhibition of XOD is a major treatment for gout, and biflavonoids have been found to

Mass spectrometric characterization of the human androgen receptor ligand-binding domain expressed in Escherichia coli.

Z Zhu et al.

Biochemistry, 40(36), 10756-10763 (2001-09-06)

The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion

Preparation of highly pure daidzin on oligo-β-cyclodextrin-Sepharose HP and investigation of chromatographic behavior of isoflavones by molecular docking.

Li Yang et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1773-1780 (2011-05-24)

A novel method using column chromatography on oligo-β-cyclodextrin-Sepharose HP for the preparation of high purity daidzin from crude soybean samples was proposed in this work. The isoflavone of daidzin in sample A and B was purified under the optimum mobile

Preparative separation of helvolic acid from the endophytic fungus Pichia guilliermondii Ppf9 by high-speed counter-current chromatography.

Jianglin Zhao et al.

World journal of microbiology & biotechnology, 28(3), 835-840 (2012-07-19)

High-speed counter-current chromatography (HSCCC) was applied for preparative separation of helvolic acid from the crude extract of the endophytic fungus Pichia guilliermondii Ppf9, associated with the medicinal plant Paris polyphylla var. yunnanensis for the first time. The two-phase solvent system

Isolation and characterization of microcystins from laboratory cultures and environmental samples of Microcystis aeruginosa and from an associated animal toxicosis.

L A Lawton et al.

Natural toxins, 3(1), 50-57 (1995-01-01)

Six microcystins were identified in a laboratory culture of the cyanobacterium (blue-green alga) Microcystis aeruginosa PCC 7813 using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). The toxins were purified and further characterized by amino

Quality assessment of traditional herbal formula, Hyeonggaeyeongyo-tang through simultaneous determination of twenty marker components by HPLC-PDA and LC-MS/MS.

Chang-Seob Seo et al.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 28(4), 427-439 (2020-04-11)

Simultaneous analysis of 20 marker components (gallic acid, cimifugin, geniposide, paeoniflorin, ferulic acid, nodakenin, narirutin, naringin, neohesperidin, arctiin, baicalin, oxypeucedanin hydrate, wogonoside, baicalein, arctigenin, glycyrrhizin, wogonin, pulegone, decursin, and decursinol angelate) for quality assessment of the traditional herbal formula, Hyeonggaeyeongyo-tang

Rapid isolation procedure for Δ9-tetrahydrocannabinolic acid A (THCA) from Cannabis sativa using two flash chromatography systems.

Ariane Wohlfarth et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(28), 3059-3064 (2011-09-29)

Two isolation procedures for Δ9-tetrahydrocannabinolic acid A (THCA), the biogenetic precursor in the biosynthesis of the psychoactive Δ9-tetrahydrocannabinol (THC) in the cannabis plant, are presented. Two flash chromatography systems that can be used independently from each other were developed to

Solid-phase preparation of a pilot library derived from the 2,3,4,5-tetrahydro-1H-benzo[b]azepin-5-amine scaffold.

Damien Boeglin et al.

Journal of combinatorial chemistry, 9(3), 487-500 (2007-03-16)

A convenient and reliable solid-phase strategy for the synthesis of di- and trisubstituted benzazepine derivatives was developed. 5-Amino-1-tert-butoxycarbonyl-2,3,4,5-tetrahydro-1H-benzo[b]azepine and 5-amino-1-tert-butoxycarbonyl-7-bromo-2,3,4,5-tetrahydro-1H-benzo[b]azepine G-protein coupled receptor-targeted (GPCR-targeted) scaffolds were efficiently synthesized in a six-step solution-phase process, immobilized on the acid-labile FMPB-AM resin, and

Development of a high performance liquid chromatography method and a liquid chromatography-tandem mass spectrometry method with pressurized liquid extraction for simultaneous quantification and confirmation of cyromazine, melamine and its metabolites in foods of animal origin.

Huan Yu et al.

Analytica chimica acta, 682(1-2), 48-58 (2010-11-09)

Simple and sensitive methods have been developed for simultaneous detection of cyromazine, melamine and their metabolites (ammeline, ammelide and cyanuric acid) in samples of animal origins. These include a high performance liquid chromatography (HPLC) method and a liquid chromatography-tandem mass

Determination of biotin following derivatization with 2-nitrophenylhydrazine by high-performance liquid chromatography with on-line UV detection and electrospray-ionization mass spectrometry.

Chikako Yomota et al.

Journal of chromatography. A, 1142(2), 231-235 (2007-01-20)

Currently, biotin is typically determined in Japan using a microbiological method. Such microbiological assays are sensitive, but they are not always highly specific and are also rather tedious and time-consuming. In the present study, RP-HPLC and LC-MS methods for the

Multiresidue determination of acidic pesticides in water by HPLC-DAD with confirmation by GC-MS using conversion to the methyl ester with trimethylsilyldiazomethane.

Thomas W Moy et al.

Journal of chromatographic science, 41(7), 343-349 (2003-08-12)

A multiresidue pesticide methodology is studied and results for acidics are reported here, with base/neutral to follow. This work studies a literature procedure as a possible general approach to many pesticides and potentially other analytes that are considered to be

Identification of Phytoconstituents in Leea indica (Burm. F.) Merr. Leaves by High Performance Liquid Chromatography Micro Time-of-Flight Mass Spectrometry.

Deepika Singh et al.

Molecules (Basel, Switzerland), 24(4) (2019-02-20)

Leea indica (Vitaceae) is a Southeast Asian medicinal plant. In this study, an ethyl acetate fraction of L. indica leaves was studied for its phytoconstituents using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-microTOF-Q-MS/MS) analysis. A total of 31 compounds of different

Anti-proliferative and apoptotic properties of a peptide from the seeds of Polyalthia longifolia against human cancer cell lines.

S Rupachandra et al.

Indian journal of biochemistry & biophysics, 51(2), 127-134 (2014-07-02)

The peptides produced enzymatically from various plants have shown various biological activities including cytotoxicity. Different types of cytotoxic peptides have been reported from the seeds and leaves of Violaceae, Rubiaceae and Annonaceae families. In this study, we report purification and

Utilising Magnetically Isolated Lysosomes for Direct Quantification of Intralysosomal Drug Concentrations by LC-MS/MS Analysis: An Investigatory Study With Imipramine.

Laura Francis et al.

Journal of pharmaceutical sciences, 109(9), 2891-2901 (2020-06-07)

Lysosomes are acidic intracellular organelles that can extensively sequester basic lipophilic drugs due to pH and membrane partitioning, and therefore may significantly influence subcellular drug concentrations. Current in vitro methods for lysosomal drug sequestration evaluation typically lack the ability to accurately

Chromogranin A from bovine adrenal medulla: molecular characterization of glycosylations, phosphorylations, and sequence heterogeneities by mass spectrometry.

S H Bauer et al.

Analytical biochemistry, 274(1), 69-80 (1999-10-21)

Chromogranin A (CGA) is a member of a family of acidic glycoproteins present in endocrine and neuroendocrine tissues. One of its suggested physiological roles is being a precursor molecule for several peptide hormons. Further interest in this protein has recently

Identification and characterization of related substances in pomalidomide by hyphenated LC-MS techniques.

Ping Lu et al.

Journal of pharmaceutical and biomedical analysis, 114, 159-167 (2015-06-07)

The current study dealt with the separation, identification and characterization of related substances in pomalidomide by hyphenated techniques. Complete separation was obtained with an Inertsil ODS-SP column (250 mm × 4.6 mm, 5 μm) by linear gradient elution using a

Development and validation of a high-throughput based on liquid chromatography with ultraviolet absorption and mass spectrometry detection method for quantitation of cichoric acid in Echinacea purpurea aerial-based dietary supplements.

Pei Chen

Journal of AOAC International, 89(3), 612-618 (2006-06-24)

A new, rapid, and reproducible reversed-phased liquid chromatography (LC) method with ultraviolet (UV) absorption and/or mass spectrometry (MS) detection has been developed and validated for quantitation of cichoric acid, a major constituent of Echinacea spp. The method involves the use

9-fluorenylmethyl chloroformate as a fluorescence-labeling reagent for derivatization of carboxylic acid moiety of sodium valproate using liquid chromatography/tandem mass spectrometry for binding characterization: a human pharmacokinetic study.

Bahareh Mohammadi et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 880(1), 12-18 (2011-12-17)

In High Performance Liquid Chromatographic (HPLC) determination of chemicals with acidic functions, different labeling agents are used to improve sensitivity of the assay. 9-Fluorenylmethyl chloroformate (FMOC-Cl), on the other hand, is a suitable labeling agent, which reacts with both primary

Characterization of proanthocyanidins in stems of Polygonum multiflorum Thunb as strong starch hydrolase inhibitors.

Hongyu Wang et al.

Molecules (Basel, Switzerland), 18(2), 2255-2265 (2013-02-23)

Characterization of polyphenolic compounds in the stems of P. multiflorum was conducted using HPLC, high resolution LC-MS and LC-MSn. Proanthocyanidins in particular were isolated in 4.8% yield using solvent extraction followed by Sephadex LH-20 fractionation. HPLC analysis using a diol

[Pharmacokinetics of 8-O-acetylharpagide and harpagide after oral administration of Ajuga decumbens extract in beagle dog].

Bin-Yu Wen et al.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(12), 2015-2018 (2013-09-27)

8-O-acetylharpagide and harpagide are two kinds of effective component of Ajuga decumbens extract. A sensitive LC-MS/MS method has been established for pharmacokinetics of 8-O-acetylharpagide and harpagide in beagle dog after oral administration of from A. decumbens extract. Female beagle dogs

High-throughput quantitation of amino acids in rat and mouse biological matrices using stable isotope labeling and UPLC-MS/MS analysis.

Edward Takach et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 964, 180-190 (2014-05-21)

Quantifying amino acids in biological matrices is typically performed using liquid chromatography (LC) coupled with fluorescent detection (FLD), requiring both derivatization and complete baseline separation of all amino acids. Due to its high specificity and sensitivity, the use of UPLC-MS/MS

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