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Showing 1-30 of 116 results for "M8410" within Papers
Anna J Korzekwa et al.
Animals : an open access journal from MDPI, 10(7) (2020-07-18)
The aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups
Benedikt Wefers et al.
Methods (San Diego, Calif.), 121-122, 55-67 (2017-03-07)
The generation of targeted mouse mutants is a key technology for biomedical research. Using the CRISPR/Cas9 system for induction of targeted double-strand breaks, gene editing can be performed in a single step directly in mouse zygotes. This article covers the
Yilong Miao et al.
STAR protocols, 2(1), 100298-100298 (2021-02-04)
The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide
Xue Zhao et al.
Reproduction, fertility, and development, 33(14), 772-781 (2021-11-09)
Reproductive techniques such as superovulation and in vitro fertilisation (IVF) have been widely used in generating genetically modified animals. The current gold standard for superovulation in mice is using coherent treatments of equine chorionic gonadotropin (eCG) and human chorionic gonadotropin
Rapeechai Navawongse et al.
Neurobiology of learning and memory, 131, 176-181 (2016-04-12)
Elucidating the genetic, and neuronal bases for learned behavior is a central problem in neuroscience. A leading system for neurogenetic discovery is the vinegar fly Drosophila melanogaster; fly memory research has identified genes and circuits that mediate aversive and appetitive
Kourosh Gharun et al.
EMBO reports, 18(12), 2144-2159 (2017-11-04)
Immunity to mycobacteria involves the formation of granulomas, characterized by a unique macrophage (MΦ) species, so-called multinucleated giant cells (MGC). It remains unresolved whether MGC are beneficial to the host, that is, by prevention of bacterial spread, or whether they
Clare E Hancock et al.
STAR protocols, 1(3), 100210-100210 (2020-12-31)
This protocol enables the quantification of odor-evoked calcium activity in mushroom body Kenyon cells of the Drosophila melanogaster brain at the single bouton level. We also present subsequent characterization of naive and learned odor representations in the context of olfactory
Xili Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(44), 27388-27399 (2020-10-23)
The fine balance of growth and division is a fundamental property of the physiology of cells, and one of the least understood. Its study has been thwarted by difficulties in the accurate measurement of cell size and the even greater
Takeshi Itabashi et al.
Nature methods, 6(2), 167-172 (2009-01-20)
Accurate chromosome segregation during meiosis depends on the assembly of a microtubule-based spindle of proper shape and size. Current models for spindle-size control focus on reaction diffusion-based chemical regulation and balance in activities of motor proteins. Although several molecular perturbations
Alberto Stracuzzi et al.
Biomechanics and modeling in mechanobiology, 20(2), 751-765 (2021-02-04)
Probing mechanical properties of cells has been identified as a means to infer information on their current state, e.g. with respect to diseases or differentiation. Oocytes have gained particular interest, since mechanical parameters are considered potential indicators of the success
Thi Thu Hien Bui et al.
Molecular reproduction and development, 84(5), 356-362 (2017-02-25)
Full-grown mouse antral oocytes are classified as surrounding nucleolus (SN) or not-surrounding nucleolus (NSN), depending on the respective presence or absence of a ring of Hoechst-positive chromatin surrounding the nucleolus. In culture, both types of oocytes resume meiosis and reach
Marie-Laure Volvert et al.
BMC pharmacology, 8, 10-10 (2008-06-14)
Lipid-soluble thiamine precursors have a much higher bioavailability than genuine thiamine and therefore are more suitable for therapeutic purposes. Benfotiamine (S-benzoylthiamine O-monophosphate), an amphiphilic S-acyl thiamine derivative, prevents the progression of diabetic complications, probably by increasing tissue levels of thiamine
Corina M Markodimitraki et al.
Nature protocols, 15(6), 1922-1953 (2020-05-01)
Protein-DNA interactions are essential for establishing cell type-specific chromatin architecture and gene expression. We recently developed scDam&T-seq, a multi-omics method that can simultaneously quantify protein-DNA interactions and the transcriptome in single cells. The method effectively combines two existing methods: DNA
Xuchu Ma et al.
Dalton transactions (Cambridge, England : 2003), (46)(46), 10250-10255 (2009-11-19)
Na(4)Si(4) and Na(4)Ge(4) are ideal chemical precursors for inorganic clathrate structures, clusters, and nanocrystals. The monoclinic Zintl phases, Na(4)Si(4) and Na(4)Ge(4), contain isolated homo-tetrahedranide [Si(4)](4-) and [Ge(4)](4-) clusters surrounded by alkali metal cations. In this study, a simple scalable route
Gang Liu et al.
Theriogenology, 113, 78-84 (2018-02-24)
The survival rate of vitrified-thawed ovarian tissues after autotransplantation still needs to be improved. Therefore finding an ideal cryoprectant to reduce the damage to ovaries that caused by vitrification will pave the way for application of ovary cryopreservation on clinics.
Taeyeon Kim et al.
The Journal of antimicrobial chemotherapy, 70(6), 1659-1668 (2015-03-01)
To systematically analyse the interplay between the expression of Hfq-associated small non-coding RNAs (sRNAs) and antibiotic susceptibility in Gram-negative bacteria. To identify the roles of sRNAs in the antibiotic susceptibility of Escherichia coli and Salmonella species, susceptibility tests, growth analyses
Zhong-Tao Feng et al.
Plant science : an international journal of experimental plant biology, 238, 286-296 (2015-08-11)
Recretohalophytes with specialized salt-secreting structures (salt glands) can secrete excess salts from plant, while discriminating between Na(+) and K(+). K(+)/Na(+) ratio plays an important role in plant salt tolerance, but the distribution and role of K(+) in the salt gland
Etsuo A Susaki et al.
Nature communications, 11(1), 1982-1982 (2020-04-29)
Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of the staining system, we developed a highly optimized 3D staining imaging pipeline based on CUBIC. Based on our precise characterization of
Artyom A Astafiev et al.
ACS omega, 5(21), 12527-12538 (2020-06-18)
Fluorescent particle tracking is a powerful technique for studying intracellular transport and microrheological properties within living cells, which in most cases employs exogenous fluorescent tracer particles delivered into cells or fluorescent staining of cell organelles. Herein, we propose an alternative
Lynda K McGinnis et al.
Developmental biology, 327(2), 280-287 (2009-01-03)
Oocyte maturation invokes complex signaling pathways to achieve cytoplasmic and nuclear competencies for fertilization and development. The Src-family kinases FYN, YES and SRC are expressed in mammalian oocytes but their function during oocyte maturation remains an open question. Using chemical
Xiang Yin et al.
Experimental and therapeutic medicine, 18(1), 269-277 (2019-07-02)
Platelets primarily participate in hemostasis and antimicrobial host defense. The present study aimed to investigate the effects of leucine-rich repeat flightless-interacting protein-1 (LRRFIP1) on platelet agglutination. The bacterial strain of LRRFIP1 was used to synthesize the recombinant protein and a
Johannes Dittmann et al.
Journal of the mechanical behavior of biomedical materials, 77, 764-775 (2017-08-12)
The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical
Gungun Lin et al.
Sensors (Basel, Switzerland), 15(6), 12526-12538 (2015-05-30)
We report a magnetofluidic device with integrated strong ferromagnetically-coupled and hysteresis-free spin valve sensors for dynamic monitoring of ferrofluid droplets in microfluidics. The strong ferromagnetic coupling between the free layer and the pinned layer of spin valve sensors is achieved
Yael Oran et al.
Nature communications, 12(1), 4095-4095 (2021-07-04)
Interhemispheric correlation between homotopic areas is a major hallmark of cortical physiology and is believed to emerge through the corpus callosum. However, how interhemispheric correlations and corpus callosum activity are affected by behavioral states remains unknown. We performed laminar extracellular
Angela Rouyar et al.
PloS one, 6(10), e26443-e26443 (2011-10-27)
In nature the aerial trace of pheromone used by male moths to find a female appears as a train of discontinuous pulses separated by gaps among a complex odorant background constituted of plant volatiles. We investigated the effect of such
Catherine Rouby et al.
International journal of otolaryngology, 2011, 203805-203805 (2011-11-03)
The LCOT is a self-administered test designed to assess olfactory deficits. Altogether, 525 subjects contributed to the validation. Elderly participants were well represented in this sample. In a validation study (study 1), 407 healthy and 17 anosmic volunteers between 15
Petra Kellers et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 64(Pt 8), 719-722 (2008-08-06)
The membrane-bound [NiFe] hydrogenase is a unique metalloprotein that is able to catalyze the reversible oxidation of hydrogen to protons and electrons during a complex reaction cycle. The [NiFe] hydrogenase was isolated from the photosynthetic purple sulfur bacterium Allochromatium vinosum
Gloria S Yen et al.
Journal of the American Chemical Society, 141(4), 1515-1525 (2019-01-04)
We present a method to determine the concentration of nucleic acids in a sample by partitioning it into droplets with a nonuniform volume distribution. This digital PCR method requires no special equipment for partitioning, unlike other methods that require nearly
Maaike Welling et al.
Bio-protocol, 10(11), e3645-e3645 (2021-03-05)
Mammalian embryonic development starts with a single fertilized zygote that develops into a blastocyst embryo consisting of three cell types that evolve into either embryonic or extra-embryonic tissues. Lineage tracing of these cells can provide important information about the molecular
Ronghui Li et al.
STAR protocols, 2(3), 100745-100745 (2021-08-26)
Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation
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