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Rokhsareh Meamar et al.
Cell journal, 14(3), 185-192 (2013-03-20)
Ecstasy, or 3, 4 (±) methylenedioxymethamphetamine (MDMA), is a potent neurotoxic drug. One of the mechanisms for its toxicity is the secondary release of glutamate. Mouse embryonic stem cells (mESCs) express only one glutamate receptor, the metabotropic glutamate receptor 5
Max Villa et al.
Biomedical microdevices, 12(2), 253-261 (2009-12-17)
We present optimal perfusion conditions for the growth of primary mouse embryonic fibroblasts (mEFs) and mouse embryonic stem cells (mESCs) using a microfluidic perfusion culture system. In an effort to balance nutrient renewal while ensuring the presence of cell secreted
Patient-specific stem cell lines derived from human parthenogenetic blastocysts.
E S Revazova, N A Turovets, O D Kochetkova, L B Kindarova, L N Kuzmichev, J D Janus, M V Pryzhkova
Cloning and Stem Cells null
Derek C Liberti et al.
Developmental biology, 454(2), 108-117 (2019-06-27)
Lung endoderm development occurs through a series of finely coordinated transcriptional processes that are regulated by epigenetic mechanisms. However, the role of DNA methylation in regulating lung endoderm development remains poorly understood. We demonstrate that DNA methyltransferase 1 (Dnmt1) is
Pluripotent stem cells as a source of osteoblasts for bone tissue regeneration.
Zhu, et al.
Biomaterials, 196, 31-45 (2020)
Guangyu An et al.
Stem cells (Dayton, Ohio), 28(9), 1560-1567 (2010-08-04)
B-cell regulator of immunoglobulin heavy chain transcription (Bright)/ARID3a, an A+T-rich interaction domain protein, was originally discovered in B lymphocyte lineage cells. However, expression patterns and high lethality levels in knockout mice suggested that it had additional functions. Three independent lines
Deanne J Whitworth et al.
Stem cells and development, 28(3), 151-164 (2018-11-13)
The platypus (Ornithorhynchus anatinus) is an egg-laying monotreme mammal whose ancestors diverged ∼166 million years ago from the evolutionary pathway that eventually gave rise to both marsupial and eutherian mammals. Consequently, its genome is an extraordinary amalgam of both ancestral
S Vassilieva et al.
Experimental cell research, 258(2), 361-373 (2000-07-18)
Here, we demonstrate long-term cultivation of alkaline phosphatase-positive rat embryonic stem-like (RES) cell lines. RES cells were characterized by their typical growth in highly compacted cell clusters, which were found to be sensitive against enzymatic dissociation. RES cells expressed stage-specific
Nigel G Kooreman et al.
Cell stem cell, 22(4), 501-513 (2018-02-20)
Cancer cells and embryonic tissues share a number of cellular and molecular properties, suggesting that induced pluripotent stem cells (iPSCs) may be harnessed to elicit anti-tumor responses in cancer vaccines. RNA sequencing revealed that human and murine iPSCs express tumor-associated
Daniel J Hiler et al.
Nature protocols, 11(10), 1955-1976 (2016-09-23)
Postmitotic differentiated neurons are among the most difficult cells to reprogram into induced pluripotent stem cells (iPSCs) because they have poor viability when cultured as dissociated cells. To overcome this, other protocols have required the inactivation of the p53 tumor
Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells.
Zhang, C; Jia, P; Jia, Y; Li, Y; Webster, KA; Huang, X; Achary, M; Lemanski, SL; Lemanski, LF
Journal of Cellular Biochemistry null
Kwang-Hwan Choi et al.
Stem cell reports, 13(1), 221-234 (2019-07-02)
Pig embryonic stem cells (pESCs) have been considered an important candidate for preclinical research on human therapies. However, the lack of understanding of pig pluripotent networks has hampered establishment of authentic pESCs. Here, we report that FGF2, ACTVIN, and WNT
Bo Pan et al.
Molecular human reproduction, 17(9), 550-561 (2011-03-24)
Regulation of germ-cell-specific transcription is essential for the differentiation and other physiological processes of germ cells. Germ-cell-specific transcription factors, Sohlh2 and Figla, play key roles in gametogenesis. To elucidate whether an epigenetic mechanism is involved in the controlled expression of
Reprogramming factors involved in hybrids and cybrids of human embryonic stem cells fused with hepatocytes.
Guo J, Tecirlioglu RT, Nguyen L, Koh K, Jenkin G, Trounson A
Cellular Reprogramming null
Bo Feng et al.
Nature cell biology, 11(2), 197-203 (2009-01-13)
The dominant effect of transcription factors in imparting expanded potency is best exemplified by the reprogramming of fibroblasts to pluripotent cells using retrovirus-mediated transduction of defined transcription factors. In the murine system, Oct4, Sox2, c-Myc and Klf4 are sufficient to
Induced pluripotent reprogramming from promiscuous human stemness related factors.
Nelson TJ, Martinez-Fernandez A, Yamada S, Mael AA, Terzic A, Ikeda Y
Clinical and Translational Science null
Chunli Zhao et al.
Cell research, 17(1), 80-87 (2007-01-11)
Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from nuclear transfer (NT) embryos, may play a major role in the new era of regenerative medicine. In this study we established forty nuclear transfer-ESC (NT-ESC) lines that were derived from
Francesco Neri et al.
Molecular and cellular biology, 32(4), 840-851 (2011-12-21)
Myc family members are critical to maintain embryonic stem cells (ESC) in the undifferentiated state. However, the mechanism by which they perform this task has not yet been elucidated. Here we show that Myc directly upregulates the transcription of all
Shinobu Ueda et al.
PloS one, 3(7), e2800-e2800 (2008-07-31)
The rat is a reference animal model for physiological studies and for the analysis of multigenic human diseases such as hypertension, diabetes, neurological disorders, and cancer. The rats have long been used in extensive chemical carcinogenesis studies. Thus, the rat
Federico Ferro et al.
PloS one, 7(7), e41774-e41774 (2012-07-31)
Although the role played by the core transcription factor network, which includes c-Myc, Klf4, Nanog, and Oct4, in the maintenance of embryonic stem cell (ES) pluripotency and in the reprogramming of adult cells is well established, its persistence and function
Hiroshi Watarai et al.
The Journal of clinical investigation, 120(7), 2610-2618 (2010-06-03)
NKT cells demonstrate antitumor activity when activated to produce Th1 cytokines by DCs loaded with alpha-galactosylceramide, the prototypic NKT cell-activating glycolipid antigen. However, most patients do not have sufficient numbers of NKT cells to induce an effective immune response in
Nur Izzati Mansor et al.
Bosnian journal of basic medical sciences, 21(1), 98-110 (2020-03-12)
One of the strategies in the establishment of in vitro oxidative stress models for neurodegenerative diseases, such as Alzheimer's disease (AD), is to induce neurotoxicity by amyloid beta (Aβ) peptides in suitable neural cells. Presently, data on the neurotoxicity of
C T D Kwok et al.
Stem cell research, 16(3), 651-661 (2016-04-12)
Human embryonic stem cells (hESCs) exhibit unique cell cycle structure, self-renewal and pluripotency. The Forkhead box transcription factor M1 (FOXM1) is critically required for the maintenance of pluripotency in mouse embryonic stem cells and mouse embryonal carcinoma cells, but its
Methionine sulfoxide reductase A (MsrA) protects cultured mouse embryonic stem cells from H2O2-mediated oxidative stress.
Zhang, C; Jia, P; Jia, Y; Weissbach, H; Webster, KA; Huang, X; Lemanski, SL; Achary, M; Lemanski, LF
Journal of Cellular Biochemistry null
Dao-Rong Hou et al.
Scientific reports, 6, 25838-25838 (2016-05-14)
Efficient isolation of embryonic stem (ES) cells from pre-implantation porcine embryos has remained a challenge. Here, we describe the derivation of porcine embryonic stem-like cells (pESLCs) by seeding the isolated inner cell mass (ICM) from in vitro-produced porcine blastocyst into
Hongguo Cao et al.
International journal of biological sciences, 8(4), 498-511 (2012-03-30)
Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells
Wojciech J Szlachcic et al.
Disease models & mechanisms, 8(9), 1047-1057 (2015-06-21)
Huntington disease (HD) is a brain disorder characterized by the late onset of motor and cognitive symptoms, even though the neurons in the brain begin to suffer dysfunction and degeneration long before symptoms appear. There is currently no cure. Several
Manuel Mayr et al.
Circulation research, 102(9), 1046-1056 (2008-04-05)
We have recently demonstrated that stem cell antigen 1-positive (Sca-1(+)) progenitors exist in the vascular adventitia of apolipoprotein E-deficient (apoE(-/-)) mice and contribute to smooth muscle cell (SMC) accumulation in vein graft atherosclerosis. Using a combined proteomic and metabolomic approach
Bovine induced pluripotent stem cells are more resistant to apoptosis than testicular cells in response to mono-(2-ethylhexyl) phthalate.
Lin, YC; Kuo, KK; Wuputra, K; Lin, SH; Ku, CC; Yang, YH; Wang, SW; Wang, SW; Wu, DC; Wu et al.
International Journal of Molecular Sciences null
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