MIDI67

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Showing 1-30 of 51 results for "MIDI67" within Papers

ADCC Assay Protocol

Fang L and He F

Journal of Virology (2013)

JC Virus infected choroid plexus epithelial cells produce extracellular vesicles that infect glial cells independently of the virus attachment receptor.

Bethany A O'Hara et al.

PLoS pathogens, 16(3), e1008371-e1008371 (2020-03-05)

The human polyomavirus, JCPyV, is the causative agent of progressive multifocal leukoencephalopathy (PML) in immunosuppressed and immunomodulated patients. Initial infection with JCPyV is common and the virus establishes a long-term persistent infection in the urogenital system of 50-70% of the

Secretome of adipose-derived mesenchymal stem cells promotes skeletal muscle regeneration through synergistic action of extracellular vesicle cargo and soluble proteins.

Robert Mitchell et al.

Stem cell research & therapy, 10(1), 116-116 (2019-04-07)

The mechanisms underpinning the regenerative capabilities of mesenchymal stem cells (MSC) were originally thought to reside in their ability to recognise damaged tissue and to differentiate into specific cell types that would replace defective cells. However, recent work has shown

Exosomes Promote Ovarian Cancer Cell Invasion through Transfer of CD44 to Peritoneal Mesothelial Cells.

Koji Nakamura et al.

Molecular cancer research : MCR, 15(1), 78-92 (2016-10-21)

Epithelial ovarian cancer (EOC) cells metastasize within the peritoneal cavity and directly encounter human peritoneal mesothelial cells (HPMC) as the initial step of metastasis. The contact between ovarian cancer cells and the single layer of mesothelial cells involves direct communications

Exosomes From Astrocyte Processes: Signaling to Neurons.

Arianna Venturini et al.

Frontiers in pharmacology, 10, 1452-1452 (2019-12-19)

It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks

Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage.

Evi Goulielmaki et al.

Nature communications, 11(1), 42-42 (2020-01-04)

DNA damage and metabolic disorders are intimately linked with premature disease onset but the underlying mechanisms remain poorly understood. Here, we show that persistent DNA damage accumulation in tissue-infiltrating macrophages carrying an ERCC1-XPF DNA repair defect (Er1F/-) triggers Golgi dispersal

CAD increases the long noncoding RNA PUNISHER in small extracellular vesicles and regulates endothelial cell function via vesicular shuttling.

Mohammed Rabiul Hosen et al.

Molecular therapy. Nucleic acids, 25, 388-405 (2021-09-07)

Long noncoding RNAs (lncRNAs) have emerged as biomarkers and regulators of cardiovascular disease. However, the expression pattern of circulating extracellular vesicle (EV)-incorporated lncRNAs in patients with coronary artery disease (CAD) is still poorly investigated. A human lncRNA array revealed that

Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2.

Cheng Qian et al.

International journal of oncology, 61(4) (2022-08-06)

Currently, exosomes (EXOs) are being explored as novel drug delivery carriers with greater advantages, including crossing the blood‑brain‑barrier and loading drugs. The present study utilized EXOs derived from neural stem cells (NSCs) for the delivery of molecular drugs to treat gliomas.

A new flow cytometric assay for the simultaneous analysis of antigen-specific elimination of T cells in heterogeneous T cell populations.

Christian Schütz et al.

Journal of immunological methods, 344(2), 98-108 (2009-04-01)

Novel immunosuppressive strategies are targeting for an antigen-specific deletion of T cells responsible for organ damage in autoimmunity and allograft rejection. Here, we present a new flow cytometry-based assay that allows the reliable and efficient detection of T cells that

Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells.

Frances Bach et al.

Oncotarget, 8(51), 88845-88856 (2017-11-29)

During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for these effects, we characterized NC-derived extracellular vesicles (EVs) and determined

Proteomic signatures of extracellular vesicles secreted by nonmineralizing and mineralizing human osteoblasts and stimulation of tumor cell growth.

Jess Morhayim et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(1), 274-285 (2014-11-02)

Beyond forming bone, osteoblasts play pivotal roles in various biologic processes, including hematopoiesis and bone metastasis. Extracellular vesicles (EVs) have been implicated in intercellular communication via transfer of proteins and nucleic acids between cells. We focused on the proteomic characterization

Importance of exosome depletion protocols to eliminate functional and RNA-containing extracellular vesicles from fetal bovine serum.

Ganesh Vilas Shelke et al.

Journal of extracellular vesicles, 3, doi:10-doi:10 (2014-10-16)

Extracellular vesicles (EVs), including the nano-sized exosomes, have the capacity to transfer multiple functional molecules between cells. In cell culture experiments, fetal bovine serum (FBS) is often used to supplement cell culture medium as a nutrient, but it is important

Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.

Pedram Kharaziha et al.

Oncotarget, 6(25), 21740-21754 (2015-04-07)

Docetaxel is a cornerstone treatment for metastatic, castration resistant prostate cancer (CRPC) which remains a leading cause of cancer-related deaths, worldwide. The clinical usage of docetaxel has resulted in modest gains in survival, primarily due to the development of resistance.

Tracking antigen-driven responses by flow cytometry: monitoring proliferation by dye dilution.

Paul K Wallace et al.

Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(11), 1019-1034 (2008-09-13)

Cell-tracking reagents such as the green-fluorescent protein labeling dye CFSE and the red-fluorescent lipophilic membrane dye PKH26 are commonly used to monitor cell proliferation by flow cytometry in heterogeneous cell populations responding to immune stimuli. Both reagents stain cells with

Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.

Xiaoxiao Cai et al.

Cancer medicine, 9(16), 6009-6019 (2020-07-07)

Bladder Cancer (BC) is the ninth most common tumor in the world and one of the most common malignant tumors of the urinary system. Some studies reported that miR-133b expression is reduced in BC, but whether it plays a role

Exosomes derived from cancer stem cells of gemcitabine-resistant pancreatic cancer cells enhance drug resistance by delivering miR-210.

Zhiyong Yang et al.

Cellular oncology (Dordrecht), 43(1), 123-136 (2019-11-13)

Gemcitabine (GEM)-based chemotherapy is the first-line treatment for locally advanced pancreatic cancer. GEM resistance, however, remains a significant clinical challenge. Here, we investigated whether exosomes derived from GEM-resistant pancreatic cancer stem cells (CSCs) mediate cell-cell communication between cells that are

ECM deposition is driven by caveolin-1-dependent regulation of exosomal biogenesis and cargo sorting.

Lucas Albacete-Albacete et al.

The Journal of cell biology, 219(11) (2020-10-15)

The composition and physical properties of the extracellular matrix (ECM) critically influence tumor progression, but the molecular mechanisms underlying ECM layering are poorly understood. Tumor-stroma interaction critically depends on cell communication mediated by exosomes, small vesicles generated within multivesicular bodies

Role of tumour necrosis factor receptor-1 and nuclear factor-κB in production of TNF-α-induced pro-inflammatory microparticles in endothelial cells.

S K Lee et al.

Thrombosis and haemostasis, 112(3), 580-588 (2014-07-11)

Tumour necrosis factor-α (TNF-α) is upregulated in many inflammatory diseases and is also a potent agent for microparticle (MP) generation. Here, we describe an essential role of TNF-α in the production of endothelial cell-derived microparticles (EMPs) in vivo and the

Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury.

Ying Liu et al.

Kidney diseases (Basel, Switzerland), 6(6), 422-433 (2020-12-15)

Levels of urinary microvesicles, which are increased during various kidney injuries, have diagnostic potential for renal diseases. However, the significance of urinary microvesicles as a renal disease indicator is dampened by the difficulty to ascertain their cell source. The aim

Cancer stem cells and aneuploid populations within developing tumors are the major determinants of tumor dormancy.

Anjali P Kusumbe et al.

Cancer research, 69(24), 9245-9253 (2009-12-03)

Tumor formation involves substantial cell division and genetic instability, but the relationship between quiescent cancer stem cells (CSC) and dividing progenitors in these events is poorly understood. Likewise, the implication of aneuploid cells in solid tumors is uncertain. CSCs are

Molecular mechanisms regulating CD13-mediated adhesion.

Mallika Ghosh et al.

Immunology, 142(4), 636-647 (2014-03-19)

CD13/Aminopeptidase N is a transmembrane metalloproteinase that is expressed in many tissues where it regulates various cellular functions. In inflammation, CD13 is expressed on myeloid cells, is up-regulated on endothelial cells at sites of inflammation and mediates monocyte/endothelial adhesion by

Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative

Alshaiban A, et al.

Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)

Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity.

Amy H Buck et al.

Nature communications, 5, 5488-5488 (2014-11-26)

In mammalian systems RNA can move between cells via vesicles. Here we demonstrate that the gastrointestinal nematode Heligmosomoides polygyrus, which infects mice, secretes vesicles containing microRNAs (miRNAs) and Y RNAs as well as a nematode Argonaute protein. These vesicles are

Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.

Ke Yuan et al.

The American journal of pathology, 185(1), 69-84 (2014-12-03)

Pericytes are perivascular cells localized to capillaries that promote vessel maturation, and their absence can contribute to vessel loss. Whether impaired endothelial-pericyte interaction contributes to small vessel loss in pulmonary arterial hypertension (PAH) is unclear. Using 3G5-specific, immunoglobulin G-coated magnetic

Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics.

Sybren L N Maas et al.

Journal of controlled release : official journal of the Controlled Release Society, 200, 87-96 (2015-01-04)

Nano-sized extracelullar vesicles (EVs) released by various cell types play important roles in a plethora of (patho)physiological processes and are increasingly recognized as biomarkers for disease. In addition, engineered EV and EV-inspired liposomes hold great potential as drug delivery systems.

Osteoblastic niche supports the growth of quiescent multiple myeloma cells.

Zheng Chen et al.

Blood, 123(14), 2204-2208 (2014-01-16)

The heterogeneity of multiple myeloma (MM) contributes to variable responses to therapy. In this study, we aim to correlate the heterogeneity of MM to the presence of quiescent cells using the PKH26 dye. We tracked the rare quiescent cells in

Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative.

Abdulelah Alshaiban et al.

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22(3), 230-238 (2015-01-21)

Abnormal cellular adhesion is one of the primary causes of vaso-occlusive crisis in sickle cell disease (SCD). Levels of intercellular adhesion molecule 1 (ICAM-1) and P-selectin are upregulated, resulting in increased adhesion of leukocytes and sickle red blood cells (RBCs)

Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells

Bach F, et al.

Testing, 8(51), 88845-88845 (2017)

The sphingosine 1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics.

Levi G Ledgerwood et al.

Nature immunology, 9(1), 42-53 (2007-11-27)

Although much is known about the migration of T cells from blood to lymph nodes, less is known about the mechanisms regulating the migration of T cells from tissues into lymph nodes through afferent lymphatics. Here we investigated T cell

Inhibition of HIV-1 entry by the tricyclic coumarin GUT-70 through the modification of membrane fluidity.

Kouki Matsuda et al.

Biochemical and biophysical research communications, 457(3), 288-294 (2015-01-13)

Membrane fusion between host cells and HIV-1 is the initial step in HIV-1 infection, and plasma membrane fluidity strongly influences infectivity. In the present study, we demonstrated that GUT-70, a natural product derived from Calophyllum brasiliense, stabilized plasma membrane fluidity

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