ESCs are usually co-cultured with mouse embryonic
fibroblasts during expansion to maintain the ESCs in an
undifferentiated state; removal of the fibroblasts is necessary
to initiate differentiation
formulations provide an optimal cell culture environment for many types of human
fibroblasts and mouse embryonic fibroblasts These low serum/serum-free media formulations have
been shown to grow these cells
formulations provide an optimal cell culture environment for many types of human
fibroblasts and mouse embryonic fibroblasts These low serum/serum-free media formulations have
been shown to grow these cells
hESC culture
H9 human ES cells were maintained on CF1 mouse embryonic
fibroblasts (Cat. No. PMEF-CF) seeded at a density of 40,000
fibroblasts/cm2 on tissue culture plates coated with growth
adult human fibroblasts by defined factors. Cell 131: 1-12 (2007).
5. Takahashi, K., and Yamanaka, S. Induction of pluripotent stem cells from
mouse embryonic and adult fibroblast cultures by defined
mitotically
inactivated mouse embryonic fibroblast cells.
2. After 2 passages, replate 1/3 - 1/5 of the cell suspension on the same dish without inactivated
mouse embryonic fibroblast cells.
3. After
of mouse iPS cells from mouse embryonic fibroblasts (MEFs). Mouse iPS
cells display characteristic ES cell-like morphology, stained positive for alkaline phosphatase,
expressed the correct mouse ES cell
interfere with these processes.
H9 Human embryonic stem cells were cultured in DMEM/F-12 media supplemented with bFGF either by direct co-culture on a mouse embryonic feeder
layer in Matrigel® coated (1:30
validated for the generation of
mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and
human foreskin fibroblasts (HFFs), respectively. Both mouse and human iPS cells display
conditions that were being used at
that time to grow mouse ES cells2, including the use of feeder
layers made up of mouse embryonic fibroblasts. These culture
conditions were clearly not optimal
Reprogramming Mouse Somatic Cells
SECTION 1: REPROGRAMMING MOUSE EMBRYONIC FIBROBLAST S
Important note: The following protocol has been optimized using early passage primary mouse
embryo fibroblasts (MEFs
1
Collagenase Type I for Enzymatic
Passaging of Human Embryonic
Stem Cells in HEScGRO™ Medium
Abstract
Human embryonic stem cells (hESCs) cultured in HEScGRO, a
proprietary serum-free and
important cell signaling molecule expressed during
embryonic development. Shh has been shown to be
involved in the patterning of the developing embryonic
nervous system, somite, and limbs. Particularly
/mL for the culture of human
ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-
conditioned medium (for feeder-free human ES cell culture).
of mouse iPS cells from mouse embryonic
fibroblasts (MEFs). Mouse iPS cells display characteristic ES cell-like morphology, stained positive
for alkaline phosphatase, expressed the correct mouse ES
CEllS www.millipore.com
22
Primary Mouse Embryo Fibroblasts
Many embryonic stem cell culture protocols necessitate the use of primary
mouse embryo fibroblast (PMEF) cells. In these protocols, ES
essential for mouse
embryo development.10,11 In embryonic tissues or
in cells derived from these embryos, Src, Fyn and
Lyn kinase activities are greatly enhanced. In
Csk-deficient mouse embryonic fibroblasts
and migration are severely impaired in
p130CAS-deficient primary fibroblasts,13,14 suggesting a
crucial role of p130CAS in embryonic development,
particularly in cardiovascular development and in
cytoskeletal
cells.
Anti-Fibronectin antibody, Mouse monoclonal
specifically recognizes Fibronectin from plasma and in
the pericellular extracellular matrix of cultured
fibroblasts from human1-2 and mouse3 origin.
in a 500 mL format and may be used for routine mouse embryonic stem cell culture.
pricing
PMEF-CFL EmbryoMax Primary Mouse Embryonic Fibroblasts pricing
SF008 ESGRO Complete Gelatin Solution
macrophages and lymphoid and myeloid
leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus,
and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition
Recombinant Mouse Fibroblast Growth Factor 8c
(FGF-8c) is measured by its ability to stimulate
3H-thymidine incorporation in a confluent quiescent
culture of NR6R-3T3 fibroblasts.14, 15
including mouse and human. Here we show that co-transduction of
the viruses from this set induces reprogramming in human foreskin
fibroblast BJ cells grown on a mouse embryonic fibroblast feeder
layer
immunized with partially purified mouse tenascin.
Monoclonal Anti-Mouse Tenascin1 reacts specifically
with mouse tenascin. In supernatants of cultured mouse
fibroblasts, the antibody precipitates two
(LIF) from mouse, a 20 kDa
protein containing 180 amino acid residues, is a
multifunctional glycoprotein that induces macrophage
differentiation.1 LIF is produced by T cells, fibroblasts,
liver, and
: DBA-2N embryonic stem
cells passage 67 on mouse embryonic
fibroblast cells.
Figure 3: Offspring born after injection of
FVB/N #17 passage 18 ES cells into
C57Bl/6N blastocysts. Left mouse shows
100