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Showing 1-30 of 185 results for "P1291" within Papers
Marie-Luise Winz et al.
Nature communications, 10(1), 563-563 (2019-02-06)
Ribosome-associated quality control (RQC) pathways monitor and respond to ribosome stalling. Using in vivo UV-crosslinking and mass spectrometry, we identified a C-terminal region in Hel2/Rqt1 as an RNA binding domain. Complementary crosslinking and sequencing data for Hel2 revealed binding to
David Bradley et al.
Cell reports, 34(2), 108602-108602 (2021-01-14)
Protein kinases lie at the heart of cell-signaling processes and are often mutated in disease. Kinase target recognition at the active site is in part determined by a few amino acids around the phosphoacceptor residue. However, relatively little is known
Yi-Chuan Li et al.
Science advances, 7(3) (2021-02-02)
The Cdk8 kinase module (CKM) in Mediator, comprising Med13, Med12, CycC, and Cdk8, regulates RNA polymerase II transcription through kinase-dependent and -independent functions. Numerous pathogenic mutations causative for neurodevelopmental disorders and cancer congregate in CKM subunits. However, the structure of
Gopalakrishna Poornima et al.
Nucleic acids research, 44(19), 9358-9368 (2016-11-02)
Regulation of translation plays a critical role in determining mRNA fate. A new role was recently reported for a subset of RGG-motif proteins in repressing translation initiation by binding eIF4G1. However the signaling mechanism(s) that leads to spatial and temporal
Julie P M Viala et al.
Methods in molecular biology (Clifton, N.J.), 1615, 221-232 (2017-07-02)
The discovery of protein-protein interaction networks can lead to the unveiling of protein complex(es) forming cellular machinerie(s) or reveal component proteins of a specific cellular pathway. Deciphering protein-protein interaction networks therefore contributes to a deeper understanding of how cells function.
Scott W Stevens
Methods in molecular biology (Clifton, N.J.), 488, 65-84 (2008-11-05)
Recently developed affinity purification methods have revolutionized our understanding of the higher-ordered structures of multisubunit, often low-abundance macromolecular complexes, including ribonucleoproteins (RNPs). Often, purification by classical, non-affinity-based techniques subjects salt-labile complexes to an ionic strength incompatible with the integrity of
Vladimir Yong-Gonzales et al.
PloS one, 7(12), e51540-e51540 (2013-01-04)
The Smc5-Smc6 complex in Saccharomyces cerevisiae is both essential for growth and important for coping with genotoxic stress. While it facilitates damage tolerance throughout the genome under genotoxin treatment, its function during unperturbed growth is mainly documented for repetitive DNA
Javier Encinar Del Dedo et al.
Developmental cell, 43(5), 588-602 (2017-11-28)
Oxysterol binding protein-related proteins (ORPs) are conserved lipid binding polypeptides, enriched at ER contacts sites. ORPs promote non-vesicular lipid transport and work as lipid sensors in the context of many cellular tasks, but the determinants of their distinct localization and
Sina Wittmann et al.
Nature communications, 8, 14861-14861 (2017-04-04)
Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for
Dexian Luo et al.
The Plant cell, 24(10), 4135-4148 (2012-10-30)
Eukaryotic organisms have evolved a set of strategies to safeguard genome integrity, but the underlying mechanisms remain poorly understood. Here, we report that asymmetric leaves1/2 enhancer7 (AE7), an Arabidopsis thaliana gene encoding a protein in the evolutionarily conserved Domain of
Hamza Amine et al.
RNA biology, 18(12), 2450-2465 (2021-06-02)
Antiproliferative BTG/Tob proteins interact directly with the CAF1 deadenylase subunit of the CCR4-NOT complex. This binding requires the presence of two conserved motifs, boxA and boxB, characteristic of the BTG/Tob APRO domain. Consistently, these proteins were shown to stimulate mRNA
Seung-Joon Ahn et al.
Archives of insect biochemistry and physiology, 99(3), e21500-e21500 (2018-09-07)
CAPA and pyrokinin (PK) neuropeptides are produced from two different genes, capa and pyrokinin, respectively. In this study, we identified and characterized the capa and pyrokinin genes from the brown marmorated stink bug, Halyomorpha halys (Hemiptera). The capa gene encodes
Marija Maric et al.
Science (New York, N.Y.), 346(6208), 1253596-1253596 (2014-10-25)
Chromosome replication is initiated by a universal mechanism in eukaryotic cells, involving the assembly and activation at replication origins of the CMG (Cdc45-MCM-GINS) DNA helicase, which is essential for the progression of replication forks. Disassembly of CMG is likely to
Thomas Laboucarié et al.
EMBO reports, 18(12), 2197-2218 (2017-10-29)
Gene expression regulation is essential for cells to adapt to changes in their environment. Co-activator complexes have well-established roles in transcriptional regulation, but less is known about how they sense and respond to signaling cues. We have previously shown that
Gergana Shipkovenska et al.
eLife, 9 (2020-06-04)
Heterochromatic domains containing histone H3 lysine 9 methylation (H3K9me) can be epigenetically inherited independently of underlying DNA sequence. To gain insight into the mechanisms that mediate epigenetic inheritance, we used a Schizosaccharomyces pombe inducible heterochromatin formation system to perform a
Julie Candiracci et al.
Science advances, 5(6), eaav0184-eaav0184 (2019-06-22)
Nutrient availability has a profound impact on cell fate. Upon nitrogen starvation, wild-type fission yeast cells uncouple cell growth from cell division to generate small, round-shaped cells that are competent for sexual differentiation. The TORC1 (TOR complex 1) and TORC2
Hui Hua et al.
Molecular biology of the cell, 24(5), 578-587 (2013-01-11)
Meiosis involves two successive rounds of chromosome segregation without an intervening S phase. Exit from meiosis I is distinct from mitotic exit, in that replication origins are not licensed by Mcm2-7 chromatin binding, but spindle disassembly occurs during a transient
Chen-Chun Pai et al.
Nucleic acids research, 51(7), 3205-3222 (2023-03-24)
Chromosomal instability (CIN) drives cell-to-cell heterogeneity, and the development of genetic diseases, including cancer. Impaired homologous recombination (HR) has been implicated as a major driver of CIN, however, the underlying mechanism remains unclear. Using a fission yeast model system, we
Zhongdao Li et al.
PloS one, 6(5), e20045-e20045 (2011-06-04)
In eukaryotic cells, repair of DNA double-strand breaks (DSBs) by the nonhomologous end-joining (NHEJ) pathway is critical for genome stability. In contrast to the complex eukaryotic repair system, bacterial NHEJ apparatus consists of only two proteins, Ku and a multifunctional
Brandon M Trainor et al.
RNA (New York, N.Y.), 27(12), 1602-1616 (2021-08-29)
Stress-induced molecular damage to ribosomes can impact protein synthesis in cells, but cell-based assays do not provide a clear way to distinguish the effects of ribosome damage from stress responses and damage to other parts of the translation machinery. Here
Iram Waris Zaidi et al.
EMBO reports, 9(10), 1034-1040 (2008-08-16)
In budding yeast the cullin Rtt101 promotes replication fork progression through natural pause sites and areas of DNA damage, but its relevant subunits and molecular mechanism remain poorly understood. Here, we show that in budding yeast Mms1 and Mms22 are
Glucose depletion enables Candida albicans mating independently ofA the epigenetic white-opaque switch.
Guan, et al.
Nature Communications, 14, 2067-2067 (2023)
Abdallah Alhaj Sulaiman et al.
Scientific reports, 12(1), 10023-10023 (2022-06-16)
Rapamycin is an immunosuppressant used for treating many types of diseases such as kidney carcinomas. In yeast, rapamycin inhibits the TORC1 kinase signaling pathway causing rapid alteration in gene expression and ultimately cell cycle arrest in G1 through mechanisms that
Lisong Ma et al.
Frontiers in plant science, 4, 94-94 (2013-04-19)
Plant pathogens secrete effector proteins to promote host colonization. During infection of tomato xylem vessels, Fusarium oxysporum f. sp. lycopersici (Fol) secretes the Avr2 effector protein. Besides being a virulence factor, Avr2 is recognized intracellularly by the tomato I-2 resistance
Kresti Pecani et al.
Genetics, 204(4), 1479-1494 (2016-10-30)
B-type cyclins promote mitotic entry and inhibit mitotic exit. In Saccharomyces cerevisiae, four B-type cyclins, Clb1-4, carry out essential mitotic roles, with substantial but incomplete overlap of function among them. Previous work in many organisms has indicated that B-type cyclin-dependent
Meng Wang et al.
Molecular biology of the cell, 29(5), 597-609 (2018-01-13)
In Saccharomyces cerevisiae, it is well established that Hof1, Cyk3, and Inn1 contribute to septum formation and cytokinesis. Because hof1∆ and cyk3∆ single mutants have relatively mild defects but hof1∆ cyk3∆ double mutants are nearly dead, it has been hypothesized
Ana M Matia-González et al.
STAR protocols, 2(4), 100929-100929 (2021-11-13)
In vivo characterization of RNA-protein interactions is the key for understanding RNA regulatory mechanisms. Herein, we describe a protocol for detection of proteins interacting with polyadenylated RNAs in the yeast Saccharomyces cerevisiae. Proteins are crosslinked to nucleic acids in vivo by
Xiao P Peng et al.
PLoS genetics, 14(1), e1007129-e1007129 (2018-01-24)
Smc5/6, a member of the conserved SMC family of complexes, is essential for growth in most organisms. Its exact functions in a mitotic cell cycle are controversial, as chronic Smc5/6 loss-of-function alleles produce varying phenotypes. To circumvent this issue, we
Rahul S Samant et al.
Nature, 563(7731), 407-411 (2018-11-16)
Protein misfolding is linked to a wide array of human disorders, including Alzheimer's disease, Parkinson's disease and type II diabetes1,2. Protective cellular protein quality control (PQC) mechanisms have evolved to selectively recognize misfolded proteins and limit their toxic effects3-9, thus
Benjamin Schuch et al.
The EMBO journal, 33(23), 2829-2846 (2014-10-17)
The exosome is a conserved multi-subunit ribonuclease complex that functions in 3' end processing, turnover and surveillance of nuclear and cytoplasmic RNAs. In the yeast nucleus, the 10-subunit core complex of the exosome (Exo-10) physically and functionally interacts with the
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