P1750

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Isolation, culture, and immunostaining of neonatal rat ventricular myocytes.

Ana Helena Macedo Pereira et al.

STAR protocols, 2(4), 100950-100950 (2021-11-26)

Isolation and culture of ventricular cardiomyocytes from neonatal rats (NRVMs) is a powerful model to study neonatal cardiac development, cell cycle regulation, and cardiac physiology and pathology in vitro. Here, we present our modified enzymatic digestion protocol followed by two-step

Prediction Equations for In Vitro Ileal Disappearance of Dry Matter and Crude Protein Based on Chemical Composition in Dog Diets.

Yoon Soo Song et al.

Animals : an open access journal from MDPI, 13(12) (2023-06-28)

The aims of this study were to determine in vitro ileal disappearance (IVID) of dry matter (DM) and crude protein (CP) in commercial dog diets and to develop equations for predicting the IVID of DM and CP in dog diets

Enhanced Uptake of Processed Bovine β-Lactoglobulin by Antigen Presenting Cells: Identification of Receptors and Implications for Allergenicity.

Malgorzata Teodorowicz et al.

Molecular nutrition & food research, 65(8), e2000834-e2000834 (2021-02-10)

β-lactoglobulin (BLG) is a major cow milk allergen encountered by the immune system of infants fed with milk-based formulas. To determine the effect of processing on immunogenicity of BLG, this article characterized how heated and glycated BLG are recognized and

A rapid HPLC-FLD method for Ochratoxin A detection in pig muscle, kidney, liver by using enzymatic digestion with MISPE extraction.

Giacomo Luci

MethodsX, 7, 100873-100873 (2020-04-18)

Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by various Aspergillus and Penicillium species with nephrotoxic, carcinogenic, immunotoxic and teratogenic effects. OTA has been found in several food commodities, including cereals, beer, wine and spices. OTA can

In Vitro Digestion and Fermentation of Microencapsulated Tributyrin for the Delivery of Butyrate.

Joseph D Donovan et al.

Journal of food science, 82(6), 1491-1499 (2017-05-10)

Butyrate possesses negative sensory qualities and is most effectively utilized in the intestine to provide energy to the colonocyte for the maintenance of intestinal health. Butyrate has also shown promise in the treatment of intestinal disorders and diseases such as

The anthocyanins in black currants regulate postprandial hyperglycaemia primarily by inhibiting α-glucosidase while other phenolics modulate salivary α-amylase, glucose uptake and sugar transporters.

Sisir Kumar Barik et al.

The Journal of nutritional biochemistry, 78, 108325-108325 (2020-01-18)

The hypoglycaemic effects of two Ribes sp. i.e., anthocyanin-rich black currants (BC) were compared to green currants (GC), which are low in anthocyanins to establish which compounds are involved in the regulation of postprandial glycaemia. We determined the effect of

Multi-Compartment, Early Disruption of cGMP and cAMP Signalling in Cardiac Myocytes from the mdx Model of Duchenne Muscular Dystrophy.

Marcella Brescia et al.

International journal of molecular sciences, 21(19) (2020-10-01)

Duchenne muscular dystrophy (DMD) is the most frequent and severe form of muscular dystrophy. The disease presents with progressive body-wide muscle deterioration and, with recent advances in respiratory care, cardiac involvement is an important cause of morbidity and mortality. DMD

Microstructure of starch-based meals with either palm or soybean oils alter in vitro starch digestibility with no major effects on glycaemic responses.

Carolina Bravo et al.

International journal of food sciences and nutrition, 71(5), 604-613 (2019-11-21)

Glycaemic response (GR) to starch-based meals depends on their food composition and microstructure. We studied the effect of palm and soybean oils on the microstructure of a solid starch-oil-gluten matrix, on the starch gelatinisation and in vitro digestibility. Additionally, a pilot

Influence of the particle size and hydrocolloid type on lipid digestion of thickened emulsions.

N Riquelme et al.

Food & function, 11(7), 5955-5964 (2020-07-02)

Hydrocolloids are used as stabilizing agents in order to enhance the physical stability of emulsions during their storage. However, they can also play an important role in nutrient release and bioavailability. In this context, the aim of this research was

Effect of Controlled Hydrothermal Treatments on Mung Bean Starch Structure and Its Relationship with Digestibility.

Muhammad Awais et al.

Foods (Basel, Switzerland), 9(5) (2020-05-28)

The changes in structure and digestion properties of mung bean starch due to hydrothermal treatment at various controlled temperatures were investigated. Results showed the increase in onset temperature (To) from 66.33 °C to 76.69 °C and decrease in enthalpies (∆Hg

Low bioaccessibility of vitamin D2 from yeast-fortified bread compared to crystalline D2 bread and D3 from fluid milks.

Tristan E Lipkie et al.

Food & function, 7(11), 4589-4596 (2016-10-14)

The assessment of the efficacy of dietary and supplemental vitamin D tends to be confounded by differences in the serum 25-hydroxyvitamin D response between vitamin D2 and vitamin D3. Serum response differences from these vitamers may be due to differences

Improving iron and zinc bioaccessibility through food-to-food fortification of pearl millet with tropical plant foodstuffs (moringa leaf powder, roselle calyces and baobab fruit pulp).

Reneè van der Merwe et al.

Journal of food science and technology, 56(4), 2244-2256 (2019-04-19)

Essential mineral (iron and zinc) deficiencies are still prevalent in the Semi-arid Tropics, where many people consume monotonous, predominantly cereal-based diets. This study aimed to evaluate the potential of including tropical plant foodstuffs high in iron and zinc (moringa leaves

Immunonutritional Protease Inhibitors from T. durum and A. sativa Display Metabolic Similarities When Assayed on Human Macrophage-like Cells.

Bartosz Fotschki et al.

International journal of molecular sciences, 22(15) (2021-08-08)

This study evaluated the immunonutritional effects caused by protease inhibitors from Avena sativa and Triticum durum to human macrophage-like cells. Macrophages were exposed (3 h) to extracts obtained from flours, and mitochondrial-associated oxygen consumption rates and inflammatory, metabolic, and proteome

Functional assessment of encapsulated citral for controlling necrotic enteritis in broiler chickens.

Yuexi Yang et al.

Poultry science, 95(4), 780-789 (2016-01-08)

Development of viable alternatives to antibiotics to control necrotic enteritis (NE) caused by Clostridium perfringensis becoming urgent for chicken production due to pessures on poultry producers to limit or stop the use of antibiotics in feed. We have previously identified

Iron bioavailability of maize (Zea mays L.) after removing the germ fraction.

Keigler, et al.

Frontiers in Plant Science, 14, 1114760-1114760 (2023)

Novel γ-cyclodextrin-metal-organic frameworks for encapsulation of curcumin with improved loading capacity, physicochemical stability and controlled release properties.

Yulu Chen et al.

Food chemistry, 347, 128978-128978 (2021-01-15)

A safe and biodegradable γ-cyclodextrin-metal-organic-frameworks (γ-CD-MOFs) was successfully synthesized by using an improved hydrothermal method. In this study, curcumin (Cur) was chosen for testing the encapsulation stability and release performance of γ-CD-MOFs. Results of the crystal structure measurement indicated that

Variability in nutrient composition and in vitro crude protein digestibility of 16 microalgae products.

Katharina Judith Wild et al.

Journal of animal physiology and animal nutrition, 102(5), 1306-1319 (2018-07-08)

The chemical composition of 16 microalgae products of four genera, Arthrospira (n = 2), Chlorella (n = 8), Nannochloropsis (n = 4) and Phaeodactylum (n = 2), was assayed to evaluate the intra- and inter-genera variation of nutrient profiles of commercial microalgae products. Crude protein was the main

The mechanism of lowering cholesterol absorption by calcium studied by using an in vitro digestion model.

Liliya Vinarova et al.

Food & function, 7(1), 151-163 (2015-10-21)

Studies in humans show that a calcium-enriched diet leads to lower cholesterol in blood serum. This phenomenon is usually explained in the literature with a reduced cholesterol absorption in the small intestine. Our study aims to clarify the effect of

In vitro anticoccidial activity of thymol, carvacrol, and saponins.

Martina Felici et al.

Poultry science, 99(11), 5350-5355 (2020-11-05)

The anticoccidial activity of thymol, carvacrol, and saponins was assessed in an in vitro model of coccidiosis. Eimeria spp. sporozoites were collected from field samples, characterized, and used for 2 different invasion assays on Madin-Darby Bovine Kidney cells (MDBK). The cells

Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion? A dynamic in vitro study.

Daniela Freitas et al.

Food chemistry, 297, 124885-124885 (2019-06-30)

Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion. Our aim was twofold. (1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis:

Integrity and stability of oral liposomes containing bile salts studied in simulated and ex vivo gastrointestinal media.

Shunwen Hu et al.

International journal of pharmaceutics, 441(1-2), 693-700 (2012-10-24)

The objective of this study was to investigate the integrtity and stability of oral liposomes containing glycocholate (SGC-Lip) in simulated gastrointestinal (GI) media and ex vivo GI media from rats in comparison with conventional liposomes (CH-Lip) composed of soybean phosphatidylcholine

Comparative effects of high pressure processing and heat treatment on in vitro digestibility of pea protein and starch.

Alexandra E Hall et al.

NPJ science of food, 6(1), 2-2 (2022-01-14)

The effects of high-pressure processing (HPP) and heat treatment on the digestibility of protein and starch in pea protein concentrate (PPC) were investigated. Samples of PPC with 5% (5 P) and 15% (15 P) protein were treated by HPP (600 MPa/5 °C/4 min) or heat

In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix.

Danijel D Milinčić et al.

Chemosphere, 252, 126568-126568 (2020-03-30)

The aim of this study was to investigate the bioaccessibility of pesticide residues in blueberries (commercial and sample from controlled field trial) from Serbia, involving the presence of a complex food matrix and to assess the potential risk to human

in vitro bioaccessibility of iron and zinc in fortified fruit beverages

Cilla, A,. Et al

International Journal of Food Science and Technology, 44(6), 1088-1092 (2009)

Influence of spray drying encapsulation on the retention of antioxidant properties and microstructure of flaxseed protein hydrolysates.

Zahra Akbarbaglu et al.

Colloids and surfaces. B, Biointerfaces, 178, 421-429 (2019-03-26)

In this research, bioactive peptides produced from flaxseed protein by alcalase, pancreatin, trypsin and pepsin, were encapsulated by spray drying. After analysis of amino acid composition and antioxidant properties of hydrolysates, the effect of spray-drying encapsulation via different maltodextrin (MD)

An Exploratory Study of the Role of Dietary Proteins in the Regulation of Intestinal Glucose Absorption.

Camille Dugardin et al.

Frontiers in nutrition, 8, 769773-769773 (2022-02-08)

Several studies have demonstrated that high protein diets improve glucose homeostasis. Nevertheless, the mechanisms underlying this effect remain elusive. This exploratory study aims to screen and compare the acute effects of dietary proteins from different sources on intestinal glucose absorption.

Water-Dispersible Phytosterol Nanoparticles: Preparation, Characterization, and in vitro Digestion.

Ao Li et al.

Frontiers in nutrition, 8, 793009-793009 (2022-02-01)

For improving solubility and bioaccessibility of phytosterols (PS), phytosterol nanoparticles (PNPs) were prepared by emulsification-evaporation combined high-pressure homogenization method. The organic phase was formed with the dissolved PS and soybean lecithin (SL) in anhydrous ethanol, then mixed with soy protein

Use of Oleogels to Replace Margarine in Steamed and Baked Buns.

Santiago Bascuas et al.

Foods (Basel, Switzerland), 10(8) (2021-08-28)

Bakery products are usually formulated with solid fats, like margarines and shortenings, which contain high levels of saturated and trans-fatty acids and have negative effects on human health. In this study, hydroxypropyl methylcellulose (HPMC) and xanthan gum (XG) were used

An enzyme complex increases in vitro dry matter digestibility of corn and wheat in pigs.

Kyu Ree Park et al.

SpringerPlus, 5, 598-598 (2016-06-02)

Two experiments were conducted to determine the effects of enzyme complex on in vitro dry matter (DM) digestibility for feed ingredients. The objective of experiment 1 was to screen feed ingredients that can be effective substrates for an enzyme complex

Preparation of human milk fat substitutes from palm stearin with arachidonic and docosahexaenoic acid: combination of enzymatic and physical methods.

Xiao-Qiang Zou et al.

Journal of agricultural and food chemistry, 60(37), 9415-9423 (2012-08-28)

Human milk fat substitutes (HMFSs) were prepared by a two-step process, namely, Lipozyme RM IM-catalyzed acidolysis of interesterified high-melting palm stearin with fatty acids from rapeseed oil and blending of the enzymatic product with the selected oils on the basis

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